Post-harvest assessment of fruit quality and shelf life of two elite tomato varieties cultivated in Bangladesh

Abstract

Background

Tomatoes are now one of the most popular nutrient-dense vegetables in terms of acreage, production, yield, commercial use, and consumption that add to the Bangladesh economy and ultimately reduce poverty. With this view, a lab investigation was carried out to analyze the effects of tomato (Sofol and Roma VF) verities on different post-harvest managements to extend shelf life as well as reduce post-harvest losses.

Results

This study confirmed that the fruit quality attributes (color, firmness, weight loss, chilling injury, total soluble solids, pH, disease incidence, disease severity, and dry matter content) were increased whereas moisture content, vitamin C, and titratable acidity decreased with the increases in storage duration. The high quality attributes such as color, firmness, weight loss, chilling injury, total soluble solids, pH, disease incidence, disease severity, and dry matter content increased, whereas moisture content, vitamin C and titratable acidity decrease of tomato were observed in Roma VF while Sofol variety had high weight loss, moisture content, titratable acidity, and vitamin C. Moreover, the highest shelf life (17 days) was recorded in Sofol than Roma VF (15 days). On the 16th day of storage, maximum disease severity (41%) and disease incidence (71%) were observed at an earthen cooling pot, whereas a significant reduction in disease severity (1%) and disease incidence (8%) were recorded in tomatoes stored at 10^oC temperature. Weight loss was the lowest (4%) in Sofol when kept at unperforated low-density polyethylene (LDPL) plastic bag as compared to Roma VF treatment (11%). Considering the physiological process, held at the low temperature (10 ^°C and 15 ^°C) and increased shelf life as well as reduced the diseases of tomatoes.

Conclusions

The investigator concluded that the findings of this study will be helpful with specific regard to long and medium-term storage, quality control, transportation, and advertising, and will also be beneficial to tomato growers of Bangladesh.

Introduction

One of the most important vegetables in terms of acreage, production, yield, commercial use, and consumption is the tomato (Solanum Lycopersicun). After potato and sweet potato, it is the most consumable vegetable crop holding the top of the canned vegetable list (Chowdhury 1979). Currently, It is cultivated all over the country including Bangladesh due to its adaptability to a wide range of soil and climate (Ahmed 1976), Hassan et al. 2010; Kalloo and Sachdev 1985). According to BBS (2012), the annual production of tomato is about 255 thousand metric tons from 23.82 thousand hectares of land with an average yield of 10.71 metric tons/ha. However, tomato is highly perishable because of its climacteric pattern of respiration (Wills et al. 2004). The fruits perishability is attributed to the increases in physiological and physico-chemical changes, such as loss of weight, respiration, transpiration, softening of pulp, sugar, and acid contents (Firmin 1997). In the peak harvesting period of tomato in Bangladesh, sub-standard post-harvest operations such as lack of proper storage, inadequate transportation and marketing, and insufficient processing and preservation facilities resulted in a glut in the market, and therefore, the growers fail to avail expected return of produce. Consequently, large volumes of harvested tomatoes are sold at throw-away prices and improper microbial decay also greatly contributes to the high post-harvest loss (Arah et al. 2015).

Maintenance of tomato’s quality during marketing is one of the major problems when the fruit is stored at ambient temperature. Pathogen attack is an important problem in the spoilage of tomato in storage. Many fungi and bacteria cause diseases in tomato fruits and result in enormous losses of quantity and quality of the fruits (Patel and Patel 1991). So, it is very important to reduce post-harvest losses of tomato along the supply chain.

The estimated post-harvest loss of tomatoes in the supply chain of Bangladesh is 30% (Hassan et al. 2010). Earlier reports suggested that post-harvest losses of tomatoes ranged from 20 to 30% (Coursey 1971) and 30 to 50% (Lashley 1984). Apart from the above, the quantitative and qualitative (nutritional) losses also occur if suitable post-harvest treatments are not applied. Hence, there is an urgent need to reduce the enormous post-harvest loss of tomatoes. Therefore, the lab experiment was undertaken with the following objectives: (i) to investigate the effects of varieties on the shelf life and post-harvest quality attributes of tomatoes in Bangladesh and (ii) to determine the effects of heat treatment, modified atmosphere packaging, and low storage temperatures on shelf life, physico-chemical changes, and microbial infection of tomatoes during storage.

Materials and methods

Experimental site and atmospheric conditions of storage room

The experiment was carried out at the Department of Horticulture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh. The temperatures and relative humidity (RH) ranges for the storage room and earthern cooling pot were 28.2 °C to 31.1 °C and 27.2 °C to 29.5 °C and the 51 to 76% and 57 to 79%, respectively and by using Digital Thermo Hygrometer (THERMO, TFA, Germany). Unperforated (12 cm × 9 cm) LDPE plastic bags were used for this treatment. Twenty-one fruits of each variety were individually kept in unperforated plastic bags and placed on laboratory table at ambient condition for observation.

Method of studying parameters

Moisture content

Ten grams of tomato pulp was weighed by Triple Beam Balance and taken in a Petri dish from each treatment out of each replication. The Petri dish was placed in an electric oven at 70 °C for 72 h until the constant weight attained. It was then cooled and weighed again. The moisture content of tomato pulp was calculated using the following formula:

Moisture content/weight losses were calculated using the following formula:

$$ \mathrm{Moisture}\ \mathrm{Content}=\frac{\mathrm{IW}-\mathrm{FW}}{\mathrm{IW}}\times 100 $$

(1)

Where IW is the initial weight of fruits (g) and FW is the final weight of fruits at every weight (g). Also, the dry matter percent of the tomato pulp is estimated from the data obtained during moisture estimation using the following formula:

$$ \mathrm{Percent}\ \mathrm{dry}\ \mathrm{matter}=\left(100-\mathrm{percent}\ \mathrm{moisture}\ \mathrm{content}\right) $$

(2)

Color and firmness, chilling injury, and disease incidence changes of fruit

The changes in the color of tomato were determined using a numerical rating scale of 1–7, where 1 = green, 2 = breaker, 3 = turning, 4 = pink, 5 = light red, 6 = red, 7 = ripe red (Wills et al. 2004). The firmness of tomato fruit was determined by hand feel using a numerical rating scale of 1–6, where 1 = hard, 2 = sprung, 3 = between sprung and eating ripe, 4 = eating ripe, 5 = overripe, and 6 = rotten. The chilling injury of the fruits during storage was qualitatively determined based on visual observation using a numerical rating scale of 1–4 (1 = no chilling injury, 2 = slight chilling injury, 3 = moderate chilling injury, 4 = severe chilling injury). The disease incidence was calculated (Zhang et al. 2014) as follows:

$$ \mathrm{Disease}\ \mathrm{incidence}\ \left(\%\right)=\left(\mathrm{number}\ \mathrm{of}\ \mathrm{infected}\ \mathrm{fruits}/\mathrm{total}\ \mathrm{number}\ \mathrm{of}\ \mathrm{fruits}\ \mathrm{at}\ \mathrm{every}\ \mathrm{date}\right)\times 100 $$

(3)

Vitamin C content

The vitamin C content of the sample was calculated by using the following formula (Ali et al. 2015)

$$ \mathrm{Vitamin}\ \mathrm{C}\ \mathrm{content}\ \left(\mathrm{mg}/100\mathrm{mg}\right)=\left(\left(\mathrm{Ts}\times \mathrm{D}\times {\mathrm{V}}_1\right)/{\mathrm{V}}_2\times \mathrm{W}\right)\times 100\Big) $$

(4)

Where T = titre, D = dye factor, V₁ = volume made up, V₂ = volume of extract, W = weight of sample. The dye factor was calculated using this formula: dye factor = (0.5/titre).

TSS and titratable acidity

Total soluble solids (TSS) content of tomato pulp was estimated using Abbe’s refractometer. A drop of tomato juice squeezed from the fruit pulp was placed on the prism of the refractometer and TSS was recorded as %Brix from a direct reading of the instrument. Temperature corrections were made using the temperature correction chart. Titratable acidity of tomato pulp was determined according to the method mentioned by Ranganna (1979).

pH

pH 7 and pH 4 buffer tablet (BDH) chemicals Ltd. Poole, England) was dissolved in water and made up to the mark of 100 ml with distilled water. For the determination of pulp pH, 4 gm of fresh pulp was taken in a conical flask containing 10 ml of distilled water. Then, the pulp was crushed thoroughly in a mortar and pestle and extract was filtered through two of cloths. The pH meter (Hannan) was calibrated by using the buffer solutions of pH 7 and pH 4 when correction for temperature was also taken into consideration. On completion of calibration, the electrode was washed twice with distilled water, rinsed with tomato juice, and dipped into the juice and the pH was recorded.

Plant materials

In this study, the freshly harvested tomato named “Sofol” and “Roma VF” varieties which were planted as winter tomato. The tomato fruits were collected from the local farmer of village Koltapara under Gouripur Upazila of Mymensingh on 28 April 2015. Tomatoes were kept in perforated low-density polyethylene (LDPE) bags (Fig. 1) for observation. A brief description of tomato varieties is given in Table 1.

Fig. 1

Ambient condition observation. a Storage of tomato fruit in earthen cooling pot b at water bath for hot water treatment c wrapped with LDPE bags

Table 1 Basic information about two tomato varieties in Bangladesh

Treatments and experimental design

The tomato variety V₁ = “Roma VF” and V₂ = “Sofol’” and each variety of eight treatments were considered and the experiment was constructed with the factorial arrangement in a complete block design with three replications presented in Table 2.

Table 2 Different treatments following in experiment

Post-harvest data collection

To assess the effect of varieties and post-harvest treatments on the quality and shelf life of tomatoes during storage period, the fruits of the experiment had been keenly observed and recorded at every 2 days on the abovementioned parameters. In this present study, different characters such as physical (color, firmness, total weight loss, moisture content and dry matter content, and chilling injury), chemical (total soluble solids (TSS), pH, titratable acidity (TA) and vitamin C content, microbial (disease incidence, disease severity, and post-harvest disease-causing organisms) characters, and shelf life of tomatoes was calculated as affected by different storage treatments.

Isolation and identification of causal pathogens

For identification of causal pathogens, a clean slide was selected and a drop of cotton blue was placed on the slide. Specimen was collected by scraping with sterilized scalpel, blade and was placed on the cotton blue. A cover slip was placed on the specimen. The surrounding area of cover slip was cleaned with soft cloth. Then the prepared slides were observed under compound microscope for identifying the pathogenic structures. After 24 h the prepared slides were made permanent by using nail polish and taken to the plant disease clinic of the Department of Plant Pathology, BAU to take photographic image of the organisms.

Shelf life

Shelf life of tomato fruits as influenced by different post-harvest treatments was calculated by counting the days required to ripe fully with retained optimum marketing and eating qualities.

Statistical analysis

The collected data on various parameters were statistically analyzed using MSTAT Statistical Package. The means for all the treatments were calculated and analysis of variances (ANOVA) for all the parameters were performed by F test. The significance of difference between the pair of means was compared by least significant difference (LSD) test at the 1% and 5% levels of probability (Gomez and Gomez 1984).

Results

Color, firmness, and total weight loss

Significant variation was found in respect of color changes of two tomato varieties during storage periods. Higher color score (5.91) was observed in Roma VF than that of Sofol (5.23) at 16th day of storage shown in Fig. 2a. The rates of changes in color were faster in treatment combinations of Roma VF with Control, Roma VF with 15 °C temperature and Roma VF with Hot water, and the rate was the slowest in the treatment combination Sofol with unperforated LDPE plastic bags (Table 3).

Fig. 2

Different effects of two tomato varieties under storage and ripening. a Changes of color, b fruits’ firmness:firmness score (1 = hard, 2 = sprung, 3 = between sprung and eating ripe, 4 = eating ripe, 5 = overripe, 6 = rotten), c total weight loss, d–e vitamin C content and titratable acidity. f Chilling injury at 10 ^°C and 15 ^°C, injury level (1 = no chilling injury, 2 = low chilling injury, 3 = moderate chilling injury, 4 = severe chilling injury)

Table 3 Combined effect of varieties and postharvest treatments on changes of color of tomato fruits during storage and ripening

Statistically highly significant variation was observed in respect to the firmness of tomato between two varieties during storage. The higher rate of firmness score (4.52) was found in Roma VF and the lower in Sofol (4.20) at the 16th day of storage presented in Fig. 2b. Post-harvest treatments exerted a significant effect on the firmness of tomatoes during storage and ripening. The maximum change in firmness (5.20) was observed in Roma VF with control treatment and minimum change in firmness (2.91) in Sofol with unperforated LDPE packaging at the 16th day of storage (Table 4). However, the variation in percent weight loss was highly significant due to the effects of variety at all the day of storage. Sofol lost more weight than Roma VF at the 4th, 6th, 8th, 10th, 12th, and 14th day, respectively; presented in Fig. 2c. The findings indicated that Roma VF was superior to Sofol in respect of reduced weight loss (Table 5).

Table 4 Combined effect of varieties and postharvest treatments on firmness of tomato during storage and ripening

Table 5 Effects of varieties on total weight loss of tomato during storage and ripening

Moisture and dry matter content

The variation was observed between the varieties in respect to moisture content. The variety Sofol had higher moisture content (89.43%) at the 12th day of storage than Roma VF (87.3%). The combination effects of variety and post-harvest treatments in respect of moisture content was found statistically highly significant at all days of storage. The highest moisture content (92.67%) was observed in Sofol with unperforated LDPE bags and the lowest was (84.62%) in Roma VF with control treatment combination at 4th and 12th DAS respectively (Table 6).

Table 6 Combined effect of varieties and postharvest treatments on moisture content and dry matter content of tomato during storage and ripening

Chilling injury and total soluble solids

In the present study, the chilling injury was examined under 10 and 15 ^°C storage. The main effects of variety were non-significant in the early part of 4 days storage and significant at the later part of 14 days storage. Sofol showed slightly better results than Roma VF in this regard. The combined effects of variety and different post-harvest treatments on chilling injury levels of tomato were significant in all cases except days 4 and 16. The highest injury was observed in V₁T₂ (Roma VF with 10 ^°C temperature) combination (Table 7).

Table 7 Combined effect of varieties and different postharvest treatments on chilling injury level of tomato during storage and ripening

The varietal difference in terms of TSS showed statistically significant at the 4th, 8th, and 12th day of storage. The variety Roma VF had higher TSS content than Sofol throughout the storage period presented in Fig. 2f. The variety Roma VF showed the highest (5.20%Brix) TSS content than Sofol (4.35%Brix) at the 4th day of storage. The combined effects showed highly significant result in terms of the TSS (Table 8). The combined effects of variety and different post-harvest treatments on chilling injury levels of tomato were significant at all cases except days 4 and 16. The highest injury was observed in V₁T₂ (Roma VF with 10 ^°C temperature) combination.

Table 8 Combined effects of varieties and postharvest treatments on total soluble solids of tomato during storage and ripening

pH of fruit juice and titratable acidity

There was a significant increase of pH in both varieties of fruits during storage; pH was increased from 4.33 to 4.44 in Roma VF while in Sofol it was 4.15 to 4.33 at 4th and 8th day of storage (Table 8). Titratable acidity was decreased with the advancement of the storage period. Acidity (%) was always wider in variety Sofol (0.46, 0.45, and 0.43) than Roma VF (0.41, 0.40, and 0.38) during the entire storage period (Fig. 2e). The interaction effect between variety and post-harvest treatments in respect of titratable acidity was not significant during the entire storage period. It was highest (0.49%) in Sofol with unperforated LDPE combination at 4th day of storage, while it was the lowest (0.30%) in Roma VF with control combination at 12th day of storage followed by Sofol with control (0.35%) and Roma VF with hot water (0.37%) (Table 9).

Table 9 Combined effect of varieties and different postharvest treatments on vitamin C content and titratable acidity of tomato

Vitamin C content

The ascorbic acid content of fruit pulp varied significantly among the fruits of the two varieties. Sofol and Roma VF contained vitamin C of 16.54 mg/100 g and 14.34 mg/100 g at the 4th day of storage, respectively, which decreases gradually with the advancement of storage period shown in Fig. 2d. Among the post-harvest treatments used in the study significantly influenced on vitamin C content of fruits during the entire storage period (Table 9).

Disease incidence and severity

Variation in respect of percent disease incidence was found highly significant between the varieties. It was observed that disease incidence increased with the advancement of the storage period between the varieties. However, the level of disease incidence was higher in Roma VF than Sofol. The lower (18.06%) and the higher (21.87%) disease incidences were recorded in Sofol at the 6th and 16th day of storage but in the case of Roma VF those values were 25.00% and 43.75%, respectively presented in Fig. 3.

Fig. 3

Effect of variety on disease incidence of tomato during storage and ripening

Variation in respect of disease severity was found to be highly significant between the varieties at all days of storage. A high disease severity level (24%) was observed in a variety of Roma VF and it was lower (0.36%) and was observed in variety Sofol. The combined effects of variety and post-harvest treatments showed a highly significant variation in terms of disease severity. On the 16th day of storage, the lowest (0.33%) disease severity was in Sofol with 10 ^°C treatment combination and the highest (57%) disease severity was in Roma VF with earthen pot combination (Table 10).

Table 10 Analysis of variance (mean square) of the data for disease incidence

Effect of diseases and pathogens from infected tomato fruits

Fusarium species is one of the common pathogens of post-harvest disease-causing rot on tomato. Fusarium can appear as saprophytes pathogens on plants. Harvested tomatoes are susceptible to infections caused by Fusarium due to its succulent epicarp which enables the fungal hyphae to penetrate deeply into the fruit. The disease symptoms include rots and softening of pulp which extends into the center of the fruit. The rotted tissue is often water-soaked and becomes covered by white, yellow, or pinkish mycelia externally while the infected tissue is discolored and appears pale brown presented in Fig. 4a.

Fig. 4

Photographs showing disease (a) symptom of Fusarium rot and (b) micro conidia of Fusarium sp.

Characteristics of Fusarium sp.

Fusarium typically produce both macro and micro conidia from slender phialides. Macro conidia are hyaline, two to several celled, often moderately curved, rather wide and thick walled, and may have a slightly blunted apical end and pedicellate basal cell. Micro conidia are one- to two-celled, hyaline, pyriform, fusiform to ovoid, straight, or curved. In the present study, most of the spores were two-celled micro conidia (Fig. 4b)

Shelf life

Shelf life is an important issue for tomato, and it varies from variety to variety. The longer shelf life was observed in Sofol (17.22 days) than in Roma VF (15.04 days) (Fig. 5a). The shelf life of tomato fruits was extended following 10 °C, 15 °C, hot water treatment, earthen cooling pot, plastic film wrap, unperforated LDPE bag, and perforated LDPE bags over control tomato, respectively (Fig. 5b).

Fig. 5

a Main effects of variety on the shelf life of tomato during storage and ripening, vertical bar indicates LSD value at the 1% level of significance. (V₁ Roma VF, V₂ Sofol) b Main effect of post-harvest treatments on the shelf life of tomato. Vertical bar indicates LSD value at the 1% level of significance

Discussion

Effect of varieties on shelf life and quality of tomato

A significant variation was obtained among the varieties in relation to shelf life extension of tomatoes. The longer shelf life was found in Sofol variety (17.22 days) than in Roma VF (15.04 days). The shortest shelf life of Roma VF was possibly due to the highest rate of disease incidence and disease severity. The Roma VF performed better than Sofol in both cases of color and firmness. It changes due to the chlorophyll degradation or qualitative and quantitative alternations of the green pigments into other pigments as reported by Salvador et al. (2007). The moisture content was found comparatively higher in Sofol than Roma VF. Higher losses of moisture content in the variety Roma VF could be due to their poor resistance mechanism. Although the percentage of weight loss is increased at storage, but the percentage of dry matter content is increased because fruit losses more moisture.

In this study, storage at 10 ^°C temperature and modified atmosphere packaging without perforations (unperforated LDPE bags) reduced the rate of color change and the highest rate (6.13) of peel color formation was found in control treatment. At the 16th day of storage, unperforated LDPE bags (4.17) and 10 °C temperature (4.88) stored fruits were pink followed by the plastic film (5.75), earthen pot (5.85), during the storage period the color of tomato changes from the breaker to red (Table 3). Unperforated LDPE packaging and low-temperature storage showed a slower rate of firmness change of tomato during ripening and storage. At the 16th day of storage, control showed the highest rate of firmness (5.10) and modified atmosphere packaging without perforation showed the lowest rate (3.29) of firmness (Table 4). Among the treatments, unperforated LDPE and plastic film wrapping were the best in terms of controlling weight loss similar to Pesis et al. (1992). Most of the wrapping papers or bags significantly reduced the percentage of physiological weight loss in the fruits noticed by Sing et al. (2001). So, it can be concluded that the weight loss of tomato during ripening may be attributed to loss by respiration and loss of water through various mechanisms.

Moreover, the decrease in moisture content during storage was also reported by Pathmanaban et al. (1995). The highest disease incidence was found in earthen pot (70.84%) whereas the lowest (8.33 and 12.50%) disease incidence was observed at 10 ^°C and 15 ^°C, respectively, after 16th day of storage (Table 9). Feng et al. (1991) observed that controlled or modified atmosphere storage controlled post-harvest diseases. So, it can be concluded that the disease severity of treated fruits was generally lower than untreated fruits.

The shelf life of tomato fruits ranges from 10.25 to 22 days. The fruits covered with perforated plastic bag showed extended shelf life by 7 days over control, which was similar to the result observed by Yantarasri et al. (1994) and Alves et al. (1998). The increase in shelf life was probably due to the reduction of various gaseous (O₂ and CO₂) exchange from the inner and outer atmosphere. The shortest and longest shelf lives were observed in control (T₁) and unperforated LDPE bags (T₇) containing fruits, respectively. In conclusion, the fruit storage at low temperatures like 10 or 15 ^°C temperature was good.

Combined effects of varieties and post-harvest treatment

The V₂T₇ (Sofol with unperforated LDPE bags) combination showed slower changes in color than all other combinations. Both varieties (Roma VF and Sofol) under hot water treatment showed highest (9.16 and 12.28%) weight loss, and the lowest (3.84 and 3.75%) weight loss was found under unperforated LDPE bags at 14th day of storage. The minimum weight losses in unperforated plastic bag wrapping of fruits obtained in the present study is supported by Tefera et al. (2007) and Martinez et al. (1997).

The moisture content of fruit was significantly affected by both variety and post-harvest treatments. At 4th day of storage, the higher moisture content (92.67 and 92.46%) were found in Sofol with unperforated LDPE packaging and plastic films wrapping respectively and lower moisture content (87.80%) was found in Roma VF with control and 10 ^°C temperature treatment. The increase in dry matter content with the advancement of storage period may be attributed to the corresponding decrease in moisture content due to osmotic withdrawal of water from pulp to peel by transpiration and evaporation. The maximum value of TSS (5.73%Brix) was observed at the 12th day of storage in unperforated LDPE sealed fruits cv. Roma VF and where the minimum value of TSS (4.33%Brix) was observed in hot water treatment in Sofol. The highest disease severity was recorded in Roma VF held at earthen pot and the lower disease severity was observed in Sofol with low-temperature storage at 10 ^°C.

At 12th day of storage, the highest pulp pH (4.83) observed in cv. Roma VF sealed with plastic film whereas, Sofol sealed with unperforated LDPE bag shown lowest pulp pH (4.47) in the same days of storage. The combined effects of variety and different post-harvest treatments in respect of titratable acidity were statistically not significant at different days of storage. Maximum vitamin C was lost when the fruits were stored at room temperature (28.2–31.1 °C). The highest vitamin C content 18.08 and 18.05 mg/100 g was recorded in Sofol with unperforated LDPE bags combination and the lowest 11.40 and 10.48 mg/100 g was recorded in Roma VF with control combination at the 8th and 12th days of storage, respectively. The longest shelf life (24 days) was observed in tomato fruits of variety Sofol when held in unperforated LDPE bags followed by 10 ^°C (22 days) and 15 ^°C (22 days). The shelf life of tomato at 10 and 15 °C were statistically identical. The modified atmosphere storage of tomato using LDPE bag enhanced shelf life, which was probably due to the reduction of the exchange of various gases (O₂, CO₂) from inner and outer atmosphere as well as slowing down the process leading to ripening by different post-harvest treatments.

Conclusions

This study was found that the post-harvest treatments caused a significant effect on color, firmness, weight loss, moisture content, dry matter content, chilling injury, total soluble solids, pH, titratable acidity, vitamin C content, disease incidence, disease severity, and shelf life. The unperforated plastic bag was discovered to be the best among all post-harvest treatments to reduce weight loss, disease incidence, and severity of disease, and extend tomato shelf life. Therefore, it can be concluded that the results of this experiment will be useful with particular reference to long- and medium-term storage, quality control, transportation and marketing, growers, and consumers. Moreover, further studies are suggested to examine the effects of other promising varieties and post-harvest treatments on shelf life, quality and safety of tomato in the agricultural supply chain in Bangladesh.