Purification and characterization of cationic peroxidase from ginger (Zingiber officinale)

Abstract

Background

Due to versatility in reaction catalyzed by peroxidases, they have potential applications in different areas in the health sciences, food industry, and diagnostic purposes. Therefore, the aim of this study is to investigate the properties of peroxidase from ginger to be meeting the perquisites of several applications.

Results

The cationic peroxidase (GPII) was purified to homogeneity by anion exchange chromatography using DEAE–Sepharose column followed by cation exchange chromatography using CM–Sepharose column and finally Sephacryl S-200 column. The molecular mass of GPII was 42 kDa. GPII shows oxidizing activity with several phenolic compounds by using H₂O₂ as the second substrate. The natural plant phenolic compounds as pyrogallol, catechol, and guaiacol were found to be excellent electron donors for the enzyme compared to other phenolic compounds. GPII exhibited K_m values of 3.1 and 7.1 mM and V_max values of 0.6 and 0.31 units/assay using H₂O₂ and guaiacol as substrates, respectively. The enzyme exhibited maximal peroxidase activity at broad pH’s 6.0–7.5 and 50 °C. GPII was thermal stable up to 50 °C and retained 66% of its activity at 70 °C after 1 h incubation. The GPII activated by most divalent cations tested and inhibited by Hg²⁺ and Cu²⁺ cations.

Conclusion

PGII could be used in several applications due to its catalytic properties, thermal stability, broad pH, and acting on several phenolic compounds.

Background

Peroxidases (EC.1.11.1.x) are decomposing hydrogen peroxide (H₂O₂) to oxidize a wide variety of phenolic compounds as well as non-phenolic compounds (Pandey et al. 2017). The non-animal plant peroxidases, belonging to Class III peroxidase, are enzymes that participate in diverse physiological and biochemical functions in higher plant cells and are, therefore, interesting objects of current biochemical research (Has-Schön et al. 2005; Pandey et al. 2017). They are implicated in various vital processes in vivo include cell wall edification, cellular growth, differentiation and development, in hormone catabolism, lignin polymerization, suberization, fruit growth and ripening, ethylene biosynthesis, plasma membrane redox systems and the generation of H₂O₂, auxin metabolism, senescence, and defense mechanisms against abiotic and biotic stress (Chen et al. 2012; Mohamed et al. 2014; Aslmoshtaghi and Shahsavar 2016; Galende et al. 2016; Julião et al. 2016).

Due to versatility in reaction catalyzed by peroxidases, and their ubiquitous nature, they have potential applications in different areas in the health sciences, food industry, and diagnostic purposes (Pandey et al. 2017; Abdel-Aty et al. 2018). Peroxidases are implicated in various applications in detoxification of recalcitrant organopollutants, decolourization of textile effluents, in bioremediation, polymer synthesis, paper and pulp industry, in development of biosensor, diagnosis kits, etc. (Lavery et al. 2010; Bhatti et al. 2012; Kalsoom et al. 2013; Sarvamangala 2014; Nouren et al. 2015; Tabassum et al. 2015; Mohamed et al. 2017; Moubasher et al. 2017).

Plants as sources of enzymes have become a promising field of study, because of the simplicity and ease of obtaining this biological material (Julião et al. 2016). Despite the variety of plant peroxidase sources, there is no previous study on peroxidase from Zingiber officinale commonly known as ginger, originated in the Indo-Malayan region, is now widely distributed across the tropics of Asia, Africa, America and Australia (Yeh et al. 2014). Ginger belonging to the family Zingiberaceae, is widely consumed as a spice used for over 2000 years in various foods and beverages (Chari et al. 2013). Ginger is reported to have several beneficial pharmacological effects (hypoglycemic, insulinotropic, and hypolipidemic) on health in humans (Huang et al. 2004) and in experimental animals (Akhani et al. 2004; Kondeti et al. 2011). Therefore, the aim of this study is to investigate the properties of peroxidase from ginger to be meeting the perquisites of several applications.

Methods

Plant

Fresh ginger (Zingiber officinale) was obtained from the local market, Cairo, Egypt.

Peroxidase assay

Peroxidase activity was determined by the method of Miranda et al. (1995). One milliliter of reaction mixture including 40 mM guaiacol, 8 mM H₂O₂, 20 mM sodium acetate buffer, pH 5.5, and 100 μl of enzyme. The absorbance was measured at 470 nm. One unit of peroxidase activity is defined as the amount of enzyme that increases the optical density of 1.0 per min under standard assay conditions.

Protein determination

Protein concentration was quantified by the method of Bradford (1976) using bovine serum albumin as standard.

Crude enzyme extract

Two grams of fresh ginger was homogenized with 20 mM Tris-HCl buffer, pH 7.2 using mortar. The homogenate was centrifuged at 12,000 rpm for 10 min in cooling centrifuge. The supernatant was designated as a crude enzyme extract and stored at − 20 °C until further analysis.

Purification of peroxidases from ginger

Unless otherwise stated, all purification steps of peroxidases from ginger were carried out at 4 °C. The crude enzyme extract was fractionated on DEAE-Sepharose column (10 × 1.2 cm i.d.) pre-equilibrated with 20 mM Tris-HCl buffer, pH 7.2 and eluted with a stepwise gradient of NaCl (0.0–0.4 M) in the same buffer. Fractions of 5.0 ml were collected at 4 °C and a flow rate of 30 ml/h. The most of peroxidase activity was eluted at 0.0 M NaCl and the remainder of peroxidase activity was eluted by different concentrations of NaCl. The peroxidase eluted at 0.0 M NaCl was dialyzed against 20 mM sodium acetate buffer, pH 5.5 and applied on CM-Sepharose column (10 × 1.2 cm i.d.) pre-equilibrated with the same buffer. The adsorbed proteins were eluted with a stepwise gradient of NaCl (0.0 to 0.4 M) in the same buffer at a flow rate of 30 ml/h, and 5.0 ml fractions were collected. The most peroxidase activity was eluted at 0.1 M NaCl and designated as GPII. The GPII was applied on Sephacryl S-200 and the enzyme eluted by 20 mM sodium acetate buffer, pH 5.5 at a flow rate of 20 ml/h, and 3.0 ml fractions were collected.

Molecular weight measurement

Molecular weight was determined by gel filtration technique using Sephacryl S-200 column. The column (93 × 1.6 cm i.d.) was calibrated with cytochrome C (12,400), carbonic anhydrase (29,000), bovine serum albumin (67,000), alcohol dehydrogenase (150,000), and β-amylase (200,000). Dextran blue (2,000,000) was used to determine the void volume (Vo). Subunit molecular weight was estimated by SDS-polyacrylamide gel electrophoresis (Laemmli 1970). SDS-denatured phosphorylase b (94,000), bovine serum albumin (67,000), ovalbumin (43,000), carbonic anhydrase (30,000), soybean trypsin inhibitor (20,000), and α-lactalbumin (14,200) were used for calibration.

Characterization of GPII

The K_m values of GPII were determined from Lineweaver–Burk plots by using different concentrations of H₂O₂ and guaiacol. The optimum pH of GPII was examined using 20 mM sodium citrate buffer (pH 4.0–4.5), sodium acetate buffer (pH 4.5–6.0), sodium phosphate buffer (pH 6.5–8.0), and Tris–HCl buffer (pH 8.0–9.0), respectively. The optimum temperature of GPII was determined by incubating the reaction mixture at different temperatures ranging from 25 to 80 °C. The effect of temperature on the enzyme stability was examined by pre-incubating the enzyme for 30 min in different temperatures ranging from 25 to 80 °C prior to substrate addition, followed by cooling in an ice bath, and the remaining activity was measured. The effect of metal ions on the peroxidase activity was also determined. The enzyme was pre-incubated for 30 min at 37 °C with some metal ions individually at the final concentration indicated prior to substrate addition and the remaining activity was measured.

Statistical analysis

The data were statistically analyzed by a one-way ANOVA. The data were considered means ± S.E. (n = 3).

Results

The purification of peroxidase from ginger was summarized in Table 1. The crude enzyme extract was fractionated on DEAE-Sepharose column (10 × 1.2 cm i.d.). The most of peroxidase activity was eluted at 0.0 NaCl and five peaks with low-level peroxidase activity were eluted by different concentrations of NaCl (Fig. 1). The peroxidase eluted at 0.0 NaCl was applied to CM-Sepharose column (10 × 1.2 cm i.d.). One peak of protein with low peroxidase activity was eluted at 0.0 M NaCl and designated as PGI. The most peroxidase activity was eluted at 0.1 M NaCl and designated as cationic GPII (Fig. 2). The GPII with high peroxidase activity was applied on Sephacryl S-200, where GPII retained 61.2% of its initial activity with specific activity 13,136 units/mg protein and 11.8 purification fold (Fig. 3). In the assessment of homogeneity using SDS-PAGE, the electrophoretic profile of proteins showed that the purified GPII enzyme was migrated as a single band of molecular weight of 42 kDa (Fig. 4).

Table 1 Purification scheme of peroxidase from ginger

Fig. 1

A typical elution profile for the chromatography of ginger peroxidase on DEAE-Sepharose column (6 × 1.6 cm i.d.) previously equilibrated with 20 mM Tris-HCl buffer, pH 7.2, at a flow rate of 30 ml/h and 5 ml fractions

Fig. 2

A typical elution profile for the chromatography of 0.0 M fraction of DEAE-Sepharose of ginger peroxidase on CM-sepharose column (6 × 1.6 cm i.d.) previously equilibrated with 20 mM sodium acetate buffer, pH 5.5, at a flow rate of 30 ml/h and 5 ml fractions

Fig. 3

A typical elution profile for the chromatography of GPII CM-Sepharose fraction on a Sephacryl S-200 column (90 × 1.6 cm i.d.) previously equilibrated with 50 mM sodium acetate buffer, pH 5.5, at a flow rate of 20 ml/h and 3 ml fractions

Fig. 4

SDS-PAGE for molecular weight determination for Sephacryl S-200 GPII. 1. Standard proteins, 2. Sephacryl S-200 GPII

The activity of the purified GPII towards various substrates was summarized in Table 2. The activity towards guaiacol, considered standard substrate for class III peroxidase, was taken as 100%. Substrate assay profile of the GPII enzyme revealed that the enzyme was highly active towards pyrogallol and catechol with relative activities % of 140 and 118, respectively. However, substrates as o-dianisidine, 4-aminoantipyrin, o-phenylenediamine, and m-phenylenediamine are oxidized by GPII at slower rates with relative activities % of 32, 34, 25 and 20, respectively. The Lineweaver-Burk plots relating ginger GPII reaction velocities to H₂O₂ and guaiacol were used for measuring the Michaelis-Menton kinetics (K_m and V_max) (Fig. 5). The K_m and V_max obtained for the purified GPII were 3.1 mM and 0.6 units/assay and 7.1 mM and 0.31 units/assay for H₂O₂ and guaiacol, respectively.

Table 2 Relative activities of ginger PGII towards substrates

Fig. 5

Lineweaver-Burk plots relating ginger GPII reaction velocities to H₂O₂ and guaiacol

The effect of pH on the purified GPII was investigated at different pH’s ranged from 4.0 to 9.0. The optimum pH for GPII was found at broad pH’s 6.0–7.5 (Fig. 6). The optimum temperature for the purified GPII was 50 °C (Fig. 7). The enzyme retained 65 and 40% of its residual activity at 70 and 80 °C, respectively. GPII was thermal stable up to 50 °C and retained 75 and 55% of its activity at 70 and 80 °C for 1 h incubation, respectively (Fig. 8).

Fig. 6

pH optimum of ginger GPII. The reaction mixture contained in 1.0 ml: 8 mM H₂O₂, 40 mM guaiacol, a suitable amount of enzyme, and 20 mM sodium citrate buffer (pH 4.0–4.5), sodium acetate buffer (pH 4.5–6.0), sodium phosphate buffer (pH 6.5–8.0), and Tris–HCl buffer (pH 8.0–9.0). The points are presented as mean ± S.E. (n = 3)

Fig. 7

Temperature optimum of ginger GPII. The enzyme activity was measured at various temperatures under standard assay conditions. The points are presented as mean ± S.E. (n = 3)

Fig. 8

Effect of temperature on the thermal stability of ginger GPII. The enzyme was preincubated at various temperatures for 1 h prior to substrate addition, followed by cooling in an ice bath. Activity at zero time was taken as 100% activity. The points are presented as mean ± S.E. (n = 3)

The effect of various divalent metal cations on the activity of GPII is shown in Table 3. Most of the divalent cations as Ca²⁺, Ba²⁺, Zn²⁺, Mg²⁺, Fe²⁺, and Mn²⁺ activated the purified GPII, while the Hg²⁺ and Cu²⁺ cations were a strong inhibitor for GPII even at 2 mM, but this effect was more pronounced at 5 mM.

Table 3 Effect of metal ion concentration on ginger PGII

Discussion

The purification of peroxidase from ginger showed several isoenzymes of peroxidases. The cationic GPII binding on CM-Sepharose possessed the highest peroxidase activity. The specific activity of GPII (13,136 units/mg protein) was higher than that reported for commercial horseradish peroxidase (250 units/mg solid) as a product from Simgma Company. The presence of several peroxidases in ginger proved that these enzymes belonging to the class-III peroxidase gene family which had multiple isoenzymes like several plant species (Boucoiran et al. 2000; Tognolli et al. 2002; Mohamed et al. 2011b). In this gene family, peroxidases had several roles such as seed germination, lignin formation, cell elongation, and stress defense (Shigeto and Tsutsumi 2015; Abdel-Aty et al. 2019). The molecular weight of purified GPII (42 kDa) on SDS-PAGE is compatible with that obtained by Sephacryl S-200 column. The obtained molecular weight was in accordance with peroxidases from Panaeolus sphinctrinus (42 KDa), horseradish roots (40 kDa), and chick pea (39 kDa) (Heinzkill et al. 1998; Lavery et al. 2010; Bhatti et al. 2006).

Peroxidases catalyzed the oxidation of a wide assortment of electron donor substrates; such as phenols, aromatic amines, thioanisoles, and iodide (Mirazizi et al. 2016). The different relative activities observed for GPII may be accounted for the structural differences between different substrates. Regarding the chemical nature, GPII showed the highest oxidative capacity towards phenolic compounds containing two and three hydroxy groups in their chemical structures like catechol and pyrogallol manifests a moderate activity against phenolic compounds having one hydroxyl group as guaiacol. Similarly, peroxidases from Cucumis sativus had a high affinity towards pyrogallol and guaiacol (Battistuzzi et al. 2001). On the obverse side, the GPII was poorly active towards aromatic amines such as o-dianisidine, aminoantipyrin, and phenylenediamines. On the contrast, peroxidase POII from Adalia orange had low activity towards catechol compared with o-dianisidine and o-phenylenediamine (Mohamed et al. 2008). The results showed that the natural plant phenolic compounds as pyrogallol, catechol, and guaiacol were found to be excellent electron donors for GPII compared to other phenolic compounds. Therefore, GPII could be used as an antioxidant enzyme for preventing the oxidative damage by removal of the toxic H₂O₂, which oxidized the phenolic compounds.

While H₂O₂ theorize as a suicide substrate for peroxidases; the hydrogen donor substrate may keep the peroxidase active site safe from the inactivating action of H₂O₂ (Shukla et al. 2016). Therefore, the K_m and V_max of purified GPII was determined for H₂O₂ and guaiacol. A wide range of K_m and V_max values have been reported for other peroxidases from different plants. Taking guaiacol into consideration as a substrate, various K_m and V_max values were reported for Euphorbia tirucalli peroxidase (K_m 4.13 mM and V_max of 0.627 units/assay) (Shukla et al. 2016), umbu peroxidase (K_m 6.83 mM and V_max 4.16 units/assay) (Pinto et al. 2015) and peels of Citrus reticulata var. (K_m 0.66 mM and V_max of 380 units/assay) (Nouren et al. 2013). Using H₂O₂ as peroxidase substrate revealed K_m/V_max values of 1.8 mM/20 units/assay and 0.026 mM/0.8 units/assay for peroxidases extracted from Gongronema latifolium (Joy and Eze 2015) and garlic Allium sativum (Osuji et al. 2014), respectively.

GPII had optimum pH at broad pH’s 6.0–7.5. Similar pH optima were reported for peroxidases obtained from rice seedlings (Padiglia et al. 1995), strawberry fruits (Civello et al. 1995), and umbu plants roots (Pinto et al. 2015). A wide variability in the optimum temperature has been observed for peroxidases from various plants. The optimum temperature for the purified GPII was 50 °C. Different temperature optima were shown for peroxidases from rosemary leaves (40 °C) (Aghelan and Shariat 2015), white Spanish broom (30 °C) (Galende et al. 2016), and fingerroot (Boesenbergia rotunda (L.) Mansf.) (40 °C) (Shank et al. 2015). GPII was thermal stable up to 50 °C. Peroxidases from Cocos nucifera were thermal stable up to 50 °C for 1 h. (Balasubramanian and Boopathy 2013). Generally, peroxidase has been reported as the most thermostable enzymes in plants (Bhatti 2007; Khatun et al. 2012; Nouren et al. 2013).

The most of metal cations (Ca²⁺, Ba²⁺, Zn²⁺, Mg²⁺, Fe²⁺, and Mn²⁺) caused enhancement for the activity of GPII. Similarly, the Ca²⁺ has an imperative impact on molecular folding and usually demonstrates stimulatory effects on plant class III peroxidases (Ros-Barceló and Pomar 2002). Mathé et al. (2010) reported that Mn²⁺ as well as Ca²⁺ acts as prosthetic groups for particular peroxidases known for their specific catalytic action. The obtained results for Ca²⁺ and Fe²⁺ are in accordance with Galende et al. (2016). In contrast, Shukla et al. (2016) show a moderate to high % inhibition using Co²⁺ (29.6%), Ni²⁺ (36.6%), and Zn²⁺ (89.7%) on the activity of peroxidase from the latex of plant Euphorbia tirucalli. Fe²⁺ at a concentration of 2 and 5 mM activated the purified GPII with relative activity % of 155 and 215, respectively. Fe²⁺ plays an important role in the peroxidase-oxidase cycle that involves the formation of ferrous POD (Fe²⁺) and the inactive compound III (oxyperoxidase Fe⁶⁺) (Yamazaki and Yokota 1973). In contrast, Fe²⁺ highly inhibited the activity of partially purified peroxidase from fingerroot (Boesenbergia rotunda (L.) retaining 52% of the activity compared to the control (Shank et al. 2015). Hg²⁺ was a strong inhibitor for GPII, whereas Hg²⁺ interacts with sulphydeyl groups of peroxidase resulted in inhibition of enzyme activity as reported by Einollahi et al. (2006). The peroxidase of Ficus sycomorus was inhibited by Hg²⁺ and Cu² (Mohamed et al. 2011a).

Conclusions

The cationic peroxidase (GPII) was purified and characterized from ginger (Zingiber officinale), which is widely used as a spice and folk medicine. The natural plant phenolic compounds as pyrogallol, catechol, and guaiacol were found to be excellent electron donors for the enzyme compared to other phenolic compounds. PGII could be used in several applications due to its catalytic properties, thermal stability, broad pH, and acting on various phenolic compounds.