Purification and characterization of cationic peroxidase from ginger (Zingiber officinale)

Bulletin of the National Research Centre

Table 1 Purification scheme of peroxidase from ginger

Step	Total activity (Unit^*)	Total protein (mg)	Specific activity (units mg^− 1 protein)	Fold purification	% Recovery
Crude extract	5368	4.82	1113	1	100
DEAE-Sepharose
0.0 M NaCl	4574	2.44	1874	1.7	85.2
CM-Sepharose
0.0 M NaCl CM (GPI)	704	0.8	880	0.8	13.1
0.1 M NaCl CM (GPII)	3578	0.87	4112	3.7	66.7
Sephacryl S-200
GPII	3284	0.25	13,136	11.8	61.2

^*One unit of peroxidase activity is defined as the amount of enzyme that increases the optical density 1.0 per min under standard assay conditions