Nutritional impact of inclusion of garlic (Allium sativum) and/or onion (Allium cepa L.) powder in laying hens’ diets on their performance, egg quality, and some blood constituents

Abstract

Background

Many studies mentioned that using medicinal herbs and plants as feed additives to ruminants seems to be a recent trend depending on the availability and their cost but using them with monogastric animals and birds, as pharmaceutical tool, is available. As an example, the importance of garlic or onion in recent years, thanks to a wide range of useful properties, has been increasingly used as an additive in nutrition and protection of farm animals. Their action was manifested in a reduced expanding range of pathogenic microorganisms in the digestive tract, which resulted in the rapid growth of poultry, efficient digestion, and increased immunity and health of poultry.

Methods

One hundred and eight 30-week-old laying hens were randomly divided into six dietary treatment groups {G₁ control, G₂ contained 0.5% garlic powder (GP), G₃ contained 1% GP, G₄ contained 1% onion powder (OP), G₅ contained 1% OP + 0.5% GP, and G₆ contained 1% OP + 1% GP}. Each group included 18 hens in six replicates (3 birds/each). So, this work carried out to investigate the impact of incorporating garlic powder (GP) and/or onion powder (OP) in laying hens’ diets on their performance, egg quality, and some blood constituents.

Results

Incorporating GP, OP, and the mixture of them in laying hen diets had no significant effect on the average egg weight and consumption/hen/day throughout the three stages of egg collection, but had a significant improvement in the number of eggs/hen, percentage of egg production, egg mass/hen, and feed conversion. Inclusion of GP, OP, or the mixture of them in laying hen diets had no significant effect on the shape index, Haugh unit, albumin, and shell percentages; also, an insignificant increase for shell thickness was observed, but egg weight increases (P < 0.05). Incorporation of GP, OP, and the mixture of them significantly decreased (P < 0.05) total cholesterol concentration. Also, an inclusion of 0.5% GP, 1% GP, and 1% OP + 1% GP decreased (P < 0.05) high-density lipoprotein. Inclusion of only 1% OP + 1% GP (G₆) decreased (P < 0.05) glutamic oxaloacetic transaminase; meanwhile, supplementation of 1% OP, 1% OP + 0.5% GP, or 1% OP + 1% GP increased (P < 0.05) creatinine.

Conclusion

From the results obtained and under conditions suitable for this study, it can be mentioned that the incorporation of garlic or onion powder or the mixture of them can be safely used with improvement in the general health of hens, egg weight, and feed conversion and decrease in the blood cholesterol.

Background

Numerous therapeutic and prophylactic properties have been ascribed to garlic (Allium sativum) and onion (Allium capa L.) for thousands of years, dating from such ancient civilizations as the Egyptians, Greeks, and Chinese (Kendler 1987).

Using medicinal herbs and plants as feed additives to ruminants seems to be a recent trend depending on the availability and their cost, but using them with monogastric animals and birds, as pharmaceutical tool, is available.

Garlic and onion have a stimulating effect on the gastric system (Dewit et al. 1979), while Rode et al. (1989) found that garlic extract has bactericidal effects against gram-negative organisms tested. Also, garlic oil had the anthelmintic activity on common poultry worms Ascaridia galli and Hetrakis gallinae (Kavindra et al. 2000).

Garlic action was manifested in a reduced expanding range of pathogenic microorganisms in the digestive tract, which resulted in the rapid growth of poultry, efficient digestion, and increased immunity and health of poultry (Kumar et al. 2010).

Garlic has beneficial effects on chickens: antimicrobial and antioxidant and reduced mortality and increased secretion in the stomach (Konjufca et al. 1997; Sivam 2001; Stanaćev et al. 2010).

The garlic contains important organic sulfurous compounds aliin, allicin, ajoene and allylpropyl disulphide and diallyl trisulphide, sallilcisteine, and others (Freeman and Kodera 1995; Kemper 2000; Mansoub 2011). Also, garlic contains enzymes (α-allinase, peroxydase, myrosinase, etc.), amino acids and their glycosides (arginine and others), vitamins (C, E, A, D, and B group vitamins), and minerals (iodine, potassium, sodium, calcium, magnesium, selenium, silicon, phosphorus) (Grela and Klebaniuk 2007; Popić 2009).

On the other hand, Hanafy et al. (1994) found that lead concentrations were reduced in muscles and liver tissues of chickens that were given garlic.

Adding garlic at different levels (0, 1000, 1500, 3000, and 10,000 mg/kg) in horse diets caused an increasing in feed palatability (Horton et al. 1991).

Abdullah et al. (2010) noticed that diets supplemented with 0.5% and 1% garlic powder caused decreasing broiler chicken feed consumption.

Garlic was reported to enhance performance of laying hens and improve egg quality when supplemented in diet (Lim et al. 2006; Yalcin et al. 2006; Khan et al. 2007).

Onion and garlic had valuable nutrients such as vitamins, minerals, and essential amino and fatty acids (Hariedy 1977). Also, onion and garlic are widely suggested to be used in animal feeding to stimulate growth (Koremura et al. 1984; Osman et al. 1997 and El-feel et al. 1999). Also, onion has a beneficial effect in lowering the level of cholesterol in blood plasma or serum of domestic fowls (Abdo et al. 1983 and El-Nawawi 1991).

Onion bulbs possess numerous organic sulfur compounds including trans-S-(1-propenyl) cysteine sulfoxide, S-methyl-cysteine sulfoxide, spropylcycteine sulfoxides, and cycloallicin, flavinoids, phenolic acids, sterols including cholesterol, stigma sterol, b-sitosterol, saponins, sugars, and a trace of volatile oil compounds mainly of sulfur compounds (Melvin et al. 2009). Most of the plant parts contain compounds with proven antibacterial, antiviral, antiparasitic, and anti-fungal properties and have antihypertensive, hypoglycemic, antithrombotic, antihyperlipidemic, anti-inflammatory, and antioxidant activities (Lampe 1999).

Goodarzi et al. (2013) recorded that using onion bulbs in broiler diet can decrease triglyceride and total cholesterol in blood serum.

So, this work aimed to investigate the impact of adding garlic and/or onion powders on the performance, blood plasma, and egg quality of commercial laying hens.

Methods

One hundred and eight 30-week-old commercial Bovan Brown layers were housed in individual wire cages and divided into six equal treatment groups of 18 hens each (six replicates of three birds each). All hens were kept under the same managerial, hygienic, and environmental temperature which ranged from 22 to 38 °C and humidity from 40 to 60% during over all the experimental intervals (three periods of 4 weeks each). Also, the lighting schedule was 16 h light:8 h dark/day. Hens received an iso-caloric and iso-nitrogenous (2750 ME kcal/kg and 17% CP) diets.

The experimental diets consisted of antibiotic-free layer mash formulated to meet the nutrient requirement for layers as established by NRC (1994). The compositions of different experimental rations are presented in Table 1.

Table 1 Composition and calculated chemical analysis of different experimental rations

Both garlic (Allium sativum) and onion (Allium capa L.) powders were bought from the local market and incorporated in the experimental rations as follows:

• G₁: ration diet no contained any feed additive and assigned as control ration

• G₂: ration contained 0.5% garlic powder (GP) or (5 g GP/kg feed)

• G_3: ration contained 1.0% garlic powder (GP) or (10 g GP/kg feed)

• G₄: ration contained 1.0% onion powder (OP) or (10 g OP/ kg feed)

• G₅: ration 1.0% OP + 0.5% GP or (10 g onion + 5 g garlic powder/ kg feed)

• G_6: ration contained 1.0% OP + 1.0% GP or 10 g onion + 10 g garlic powder/kg feed

Feed and water were offered ad libitum. Egg weight (EW) and egg number (EN) were recorded daily. Feed intake (FI) was recorded weekly, while feed conversion ratios (FCR) were calculated.

Egg quality parameters were measured using 36 eggs (6 eggs/each group); these involved yolk, albumen, and shell weight (%). Egg shell thickness was measured in millimeter using a micrometer as described by Günlü et al. (2003). Egg shape index was calculated as egg diameter divided by an egg length as described by Anderson et al. (2004). Yolk index was calculated as yolk height divided by the yolk diameter. Haugh unit was calculated using the calculation chart for rapid conversion of EW and albumen height as described by Doyon et al. (1986).

Analytical procedures

Chemical analysis of feed ingredients was analyzed according to AOAC (2005) methods.

At the end of the third stage of egg collection, blood samples were collected from the wing vein of laying hens using sterilized syringes and needles. Collected blood samples were centrifuged at 4000 rpm for 20 min, for the preparation of blood plasma. Plasma was kept frozen at − 18 °C for subsequent analysis.

Various blood plasma parameters were calorimetrically determined using commercial kits, following the same steps as described by the manufacturers.

Plasma total protein was determined according to Armstrong and Carr (1964) and Witt and Trendelenburg (1982) and albumin according to Doumas et al. (1971) and (Tietz 1986); globulin was calculated by subtracting the albumin value from total protein value; total cholesterol was determined according to Allain et al. (1974) and Pisani et al. (1995); alkaline phosphate activity was measured according to the method of Beliefield and Goldberg (1971); plasma glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) activities were determined as described by Reitman and Frankel (1957) and Harold (1975) and creatinine as described by Husdan (1968); high-density lipoprotein (HDL) concentration was estimated according to Assmann (1979). Also, albumin to globulin ratio (A:G ratio) was calculated.

Statistical analysis

Data collected were subjected to statistical analysis as one-way analysis of variance according to SPSS (2008). Duncan’s multiple range test (Duncan 1955) was used to separate means when the dietary treatment effect was significant according to the following model:

$$ {Y}_{ij}=\mu +{T}_i+{e}_{ij} $$

• where Y_ij = observation

• μ = overall mean

• T_i = effect of experimental rations for i = 1–6, 1 = laying hens received ration not contained any feed additive, 2 = laying hens received ration contained 0.5% garlic powder, 3 = laying hens received diet contained 1% garlic powder, 4 = laying hens received ration contained 1% onion powder, 5 = laying hens received ration contained 1% onion powder + 0.5% garlic powder and 6 = laying hens received ration contained 1% onion powder + 1% garlic powder

• e_ij = the experimental error

Results

Chemical analysis (%) of feed ingredients

Garlic powder contains 8.37% CP, 4.68% CF, 0.85% EE, 83.35% NFE, and 4.35% ash; on the other hand, the value of metabolizable energy was 2838 kcal/kg DM feed. Meanwhile, the corresponding values of the same nutrients determined in onion powder were 6.84, 5.72, 0.74, 82.35, and 4.35% for CP, CF, EE, NFE, and ash contents, respectively (Table 2).

Table 2 Chemical analysis (%) of feed ingredients

Performance

Incorporating GP, OP, and the mixture of them in laying hen diets had no significant effect on the egg weight throughout the first, second, and third (except G₆) stages of egg collection. Also, it had no significant effect on feed consumption/hen/day throughout the three stages of egg collection. On the other hand, feed conversion significantly (P < 0.05) improved (Table 3).

Table 3 Effect of dietary treatments on laying hen performance

Egg production

Data illustrated in Table 3 showed that inclusion of GP, OP, and the mixture of them in laying hen diets caused significant improvement in the number of eggs/hen, percentage of egg production, and egg mass/hen throughout the three stages of egg collection. Egg production and quality are heritable traits of major concern for breeders and consumers. To improve these characteristics, correct nutritional, management, and environmental practices are needed to enhance egg production and quality.

Egg quality

Dietary treatments significantly increased (P < 0.05) the egg weight that ranged from 60.60 to 64.60 g in comparison with the control (55.40 g), while no significant effect on shape index, Haugh unit, albumin, and shell percentages; also, an insignificant increase for shell thickness was observed (Table 4).

Table 4 Effect of dietary treatments on egg quality of laying hens

Yolk index increased (P < 0.05) for laying hen receiving ration containing 1% OP + 1% GP (G₆) compared to control (49.10 vs. 43.30). Values of yolk color were approximately in the same trend (5.00) for G₂, G_4, G₅, and G₆, which are less than those of the control group that recorded (5.20); meanwhile, G₃ recorded the lowest value (P < 0.05) of yolk color (4.80).

Blood plasma constituents

Data of Table 5 showed that incorporating 1% GP in laying hen diets (G₃) significantly (P < 0.05) decreased total protein compared to the control one, while the other dietary treatments (G₂, G₄, G₅, and G₆) are not affected. On the other hand, except for G₃, the other dietary treatments (G₂, G₄, G₅, and G₆) significantly (P < 0.05) increased albumin content. Moreover, globulin content was decreased with adding GP, OP, or the mixture of them; the lower value of globulin was recorded by laying hens that received 1% OP + 1% GP (2.35, G₆) followed by birds that received 1% GP (3.00, G₃). Laying hens fed rations contained mixture of GP + OP (G₅ and G₆) recorded the highest values of albumin to globulin ratio (P < 0.05).

Table 5 Effect of dietary treatments on some blood plasma constituents of laying hens

Lipid content

Values of high-density lipoprotein (HDL) decreased significantly (P < 0.05) with laying hens received ration 0.5% and 1% OP and 1% OP and 1% GP only. On the other hand, incorporation of GP, OP, and the mixture of them (P < 0.05) decreased total cholesterol concentration (Table 5).

Liver function

Inclusion of 1% OP + 1% GP (G₆) significantly (P < 0.05) decreased GOT content; meanwhile, the other treatments (G₂ to G₅) insignificantly decreased GOT. Incorporation of 1% OP + 1% GP (G₆) and 1% OP (G₄) insignificantly decreased GPT (Table 5).

Kidney function

Except for laying hens received 0.5% GP (G₂), the other dietary treatments significantly (P < 0.05) decreased alkaline phosphates. On the other hand, adding 0.5% or 1% GP had no significant effect. Meanwhile, supplementation of 1% OP, 1% OP + 0.5% GP, or 1% OP + 1% GP significantly (P < 0.05) increased creatinine (Table 5).

The present results cleared that inclusion of garlic powder in laying hen rations reduced creatinine concentration, because this may be related to garlic having renal protection role against some stress factors and improving its healthy condition, which is probably through its excellent antioxidant properties and highly nutritional values.

Discussion

The present results of the chemical analysis (Table 2) are within the trend noted by Nwinuka et al. (2005), Dini et al. 2008, Odebunmi et al. (2009), Otunola et al. (2010), Yalcin and Kavuncuoglu (2013), and Sajid et al. (2014) who noted that value of moisture ranged from 3.26 to 3.55%, CP ranged from 7.87 to 8.87%, CF ranged from 4.49 to 4.86%, EE ranged from 0.78 to 0.92%, and ash ranged from 3.36 to 3.73% for garlic powder. On the other hand, they found that moisture ranged from 2.68 to 2.82%, CP ranged from 6.46 to 6.81%, CF ranged from 5.58 to 5.85%, EE ranged from 0.64 to 0.83%, and ash ranged from 4.23 to 4.46% for onion powder.

Data concerning with feed intake Table 3 are in agreement with those noted by Khan et al. (2008) who found that supplementation of garlic powder had no effect on the feed consumption in native Desi laying hens. Also, Qureshi et al. (1983) did not report any differences in daily feed consumption of pullets fed diets with various garlic products at levels equal to about 50 kg/ton of added garlic bulb. Chowdhury et al. (2002) recorded that feed consumption and feed efficiency were not affected by supplementation of 0, 2, 4, 6, 8, or 10% garlic paste over the 6-week period.

The present results of feed conversion were in agreement with those attributed by Lewis and Elvin-Lewis (2003) and Demir et al. (2003) who noted an improvement in feed conversion ratio of broilers fed garlic. Meanwhile, Asrat et al. (2018) found that feed conversion was not affected by addition of garlic powder in White Leghorn hens.

It must be mentioned that eggs are delicate product which can lose quality rapidly during the period of collection to consumption. Thus, improvement in egg quality is of paramount importance in the field of production and management (Mahmoud et al. 2010).

There is a dearth of literature available concerning the beneficial effects of garlic or onion on egg quality. For example, Yalcin et al. (2006) noticed that egg weight increased when laying hens were fed 5 and 10 g/kg garlic powder. Also, Khan et al. (2007) noted that egg production increased during the 6 weeks in which 0, 2, 6, or 8% garlic powder was fed to laying hens. On the other hand, Canogullari et al. (2010) reported that egg production increased significantly by adding 1% garlic powder in the feed of laying hen. Meanwhile, Reddy et al. (1991) and Chowdhury et al. (2002) noted that the supplementation of garlic powder did not affect egg production.

Results published in Table 4 are in agreement with those found by Canogullari et al. (2010) who demonstrated that egg weight, yolk index, shell weight, shell thickness, and yolk weight did not change when laying hens fed ration containing 1% garlic powder. On the other hand, Yalcin et al. (2006) reported that the supplementation of garlic had no significant effect on egg albumin index, egg shell index, and egg Haugh unit values when laying hens fed diets containing 5 or 10 g/kg garlic powder for 22 weeks.

Values of blood plasma constituents illustrated in Table 5 are near from the results suggested by El-katcha et al. (2016) who revealed that garlic extract (allicin) supplementation at 50 mg/kg diet significantly increased albumin concentrations, while allicin supplementation at 25, 75, or 100 mg/kg had no significant effect on total protein and albumin concentrations compared with the control.

Data on lipid (Table 5) are in agreement with those found by Ahsan-Ul-Haq et al. (1999) who reported that feeding garlic at the rate of 20 g/kg significantly decreased blood cholesterol in broiler chicks. Also, Khan et al. (2008) noted that garlic powder given at the level of 8% may result in better serum cholesterol profile in laying hens. Moreover, Rehman et al. (2011) noted that a mixture of plants containing garlic as an active ingredient improved the cholesterol profile in terms of cholesterol, triglyceride, and LDL in boiler chicken serum. Konjufca et al. (1997) noticed that feeding a diet supplemented with 1.5, 3.0, or 4.5% garlic powder resulted in reduced level of plasma cholesterol. Also, Qureshi et al. (1983) found a 28–41% reduction in low-density lipoprotein (LDL) cholesterol in chickens fed diet containing 3.8% garlic paste or a solvent extract of garlic paste, the residue, or commercial garlic oil. Also, Horton et al. (1991) reported that broilers fed diet containing 10,000 mg/kg decreased their plasma cholesterol content. El-katcha et al. (2016) observed that serum total cholesterol concentration was significantly reduced by about 12.9%, 11.9%, 7.9%, and 14.2% when garlic extract was supplemented at 0.1, 0.2, 0.3, or 0.4 mg/kg diet, respectively, to broiler chickens. Reductions in total cholesterol were realized in broilers fed ration supplemented with 1 g garlic/kg feed (Mansoub 2011).

Garlic manifested hypocholesterolemic effects on chickens through inhibition of the most important enzymes that participate in the synthesis of cholesterol and lipids (Stanacev et al. 2011). Also, Konjufca et al. (1997) reported that garlic reduced plasma cholesterol by decreasing the activity of 3-hydroxy-3-methlyglutaryl reductase. Allicin has been proposed as the active compound in garlic responsible for health promotion and hypocholesterolemic benefits (Lawson 1998).

Our results of liver function (Table 5) are in agreement with El-katcha et al. (2016) who reported that higher inclusion rate of allicin (100 mg/kg) increased (P < 0.05) GPT blood serum concentration by about 3.1% in broiler. On the other hand, Kumar et al. (2013) observed that GOT decreased (P < 0.05) when broiler chicken fed diets supplemented by Allium sativum in different treatment group compared to the control group.

Generally, the mechanism by which garlic reduce plasma cholesterol concentration is not fully understood. Some of the investigators had the opinion that garlic depressed lipogenic and cholesterrogenic activities of liver enzymes such as malic enzyme, fatty acid synthase, glucose-6-phosphate dehydrogenase, and 3-hyydroxy-methyl-glutaryl-CoA (HMG-CoA) reductase (Chowdhury et al. 2002; Canogullari et al. 2010; Mahmoud et al. 2010; Khan et al. 2012). This hypothesis was demonstrated when several researchers measured cholesterol-clearing enzymes. For example, Konjufca et al. (1997) noted that feeding 3% commercial garlic powder reduced the activities of HMG-CoA reductase and cholesterol 7α-hydroxylase by 40%. Allicin has been proposed as the active compound in garlic responsible for health promotion and hypercholesterolemic benefits (Lawson 1998). In terms of the mechanism of action, it is believed to reduce cholesterol synthesis and plated aggregation and prevent thrombosis (Canogullari et al. 2010). Allicin is a volatile organic compound which has been shown to reduce serum lipids, phospholipids, and cholesterol and suppress cholesterol synthesis in chickens (Horton et al. 1991). Also, according to Mahmoud et al. (2010), the differences in the results may be due to allicin degradation, as it is an unstable compound and poorly absorbed from the gut. Additionally for that, they suggested that the garlic preparation that involved heating or solvent processes may destroy active allicin.

Conclusion

Under condition suitable for this study and from the results obtained, it can be mentioned that the incorporation of garlic or onion powder or the mixture of them can be safely used with improvement in the general health, egg weight, and feed conversion of hens and decrease in blood cholesterol.