¹H-NMR-based serum metabolomic study to evaluate the effect of asarone and metformin on experimentally induced diabetic hepatocellular carcinoma in rats

Abstract

Background

The increased prevalence of hepatocellular carcinoma (HCC) in diabetic patients has focused on the need to characterize the role of altered metabolites in liver carcinogenesis. In this study, together with the serum biochemistry and histopathological observation, ¹H nuclear magnetic resonance (¹H-NMR)-based metabolomics was carried out to study the effect of asarone and metformin in diabetic HCC rats. Intraperitoneal administration of streptozotocin (STZ; 55 mg/kg b.w.) was used to induce diabetes in male Wistar rats. Further, 2 weeks later, after confirmation of diabetes, another group received diethylnitrosamine (DEN; 200 mg/kg b.w.) to simulate the diabetic HCC condition. The combined dose of α-and β-asarone (50 µg/kg b.w. in the ratio of 1:1) and metformin HCl (250 mg/kg b.w.) treatment was orally given to the diabetic HCC rats for 18 weeks. The serum samples were subjected to ¹H-NMR-based metabolomics analysis to explore the metabolite changes at the end of the study.

Results

¹H-NMR study quantitatively distinguished the metabolites, such as pyruvate, lactate, creatine, acetate, glutamine, valine, and alanine, which varied between the diabetic HCC and normal rats. Furthermore, compared to the diabetic HCC group, the administration of asarone and metformin resulted in a substantial change in metabolite levels. Histopathological examination indicated that treatment attenuates the magnitude of the toxic effect of STZ + DEN.

Conclusions

The aberrant glucose, lipid, and amino acid metabolisms were associated with developing hepatocarcinogenesis in rats during the diabetic condition. Treatment with asarone and metformin attenuated the metabolic changes due to STZ + DEN-induced diabetic HCC.

Background

Human hepatocellular carcinoma (HCC) is one of the most common forms of primary liver cancer. It accounts for the world's third-leading cause of cancer-related death (El-Serag 2012). It usually results as a complication of preceding liver disease due to infection with the hepatitis virus, alcoholic consumption, non-alcoholic steatohepatitis (NASH), exposure to aflatoxin-contaminated food, and liver cirrhosis (Liu and Kao 2013; Venook et al. 2010; Simonetti et al. 1991). Further, epidemiological evidence suggests diabetes mellitus (DM) as one of the potential risk factors in the progression of HCC (Vecchia et al. 1997; Davila et al. 2005; Yuan et al. 2004). The current diagnostic investigations include the different biochemical profiling approaches (tumor markers mainly α-fetoprotein) along with hepatic arteriography, magnetic resonance imaging (MRI), and computed tomography (CT) by focusing on the genetic and protein variations of various cancers (Colli et al. 2006).

Magnetic resonance spectroscopy (MRS) and magnetic resonance imaging (MRI) are relatively new techniques, emerging as a powerful, robust analytical tool, particularly in medicine (Glunde et al. 2011). Nuclear magnetic resonance (NMR) spectroscopy is a robust technique that detects many metabolites present in a single experiment. This technique does not require a standard reference for each metabolite. It can be used over existing analytical methods like colorimetry, gas chromatography coupled to mass spectrometry (GC-MS), and other separation techniques. Due to its high noninvasive nature, the sample does not require pre- or post-processing (Emwas et al. 2019).

There are reports on using the NMR spectroscopy method to quantitatively measure the metabolites involved in the pathological and diagnostic progression of the diseases (Nicholson et al. 1999; Gowda et al. 2008; Griffin and Vidal-Puig 2008). The metabolic profile obtained from serum, urine, or tissue samples associated with the progression of the disease could provide a better understanding of the underlying molecular mechanism. Reports suggest promising response in elucidating the key metabolic pathways in the breast (Woo et al. 2009), brain (Petrik et al. 2006), lung (Cameron et al. 2016), prostate (Sreekumar et al. 2009), liver (Wang et al. 2011), ovarian (Denkert et al. 2006), oral (Yan et al. 2008), and pancreatic cancers (Bi et al. 2014). Various studies have also demonstrated a practical metabolomic approach for identifying potential biomarkers in diabetic patients (Yang et al. 2004; Van Doorn et al. 2007).

The changes in the glucose metabolism pathway are known to involve the progression of HCC through disturbances in the metabolism of amino acids, lipids, and energy. However, the systemic and characteristic metabolomic changes concerned with the progression of HCC during diabetic conditions remain unexplored. Furthermore, the two main pharmacologically active phytochemicals in the Acorus calamus, alpha (α)- and beta (β)-asarone, and also the extract, have been shown to have cytotoxic and hypoglycemic activities (Das et al. 2019a, b; Liu et al. 2015). On the other hand, there are reports based on epidemiological, clinical, and experimental studies suggesting that metformin reduces the cancer risk with diabetes (Li et al. 2018; Das et al. 2019a). However, there is no evidence related to asarone and metformin in support of the changes in the metabolic profile during the diabetic HCC condition. Henceforth, in the current work, we attempt to understand the metabolic profile and evaluate the impact of asarone and metformin with the changes in the key metabolites in controlling the progression of hepatocarcinogenesis during the diabetic condition via ¹H-NMR spectra. The functional significance of the analyzed metabolites for the most pertinent metabolic pathways was done based on the online Kyoto Encyclopedia of Genes and Genomes (KEGG) database and Human Metabolome Database (HMDB) (Dutta et al. 2012; Wishart et al. 2013).

Methods

Chemicals and reagents

Streptozotocin (STZ) extra pure was obtained from Sisco Research Laboratories (SRL) Pvt. Ltd., Mumbai, India. Diethylnitrosamine (DEN), deuterium oxide (D₂O), alpha- and beta-asarone were procured from Sigma-Aldrich Chemical Company (USA). Metformin HCl was obtained as a gift sample from Angels Pharma India Pvt. Ltd., Hyderabad, India. All the other reagents and chemicals were analytically graded and obtained from standard commercial suppliers.

Animal experiment

Adult male Wistar rats, weighing 150–200 g, were used for the study. Under standard environmental conditions, the animals were housed in polypropylene cages, maintained at 27 ± 2 °C with a 12-h light and dark cycle. All animal experiments were performed in agreement with the guidelines after needed approval by the Institutional Animal Ethical Committee (Approval no. 07/KLEU'SCOPH/16).

Experimental induction of diabetes and hepatocellular carcinoma

Type 1 diabetes was induced by a single intraperitoneal injection of a freshly prepared solution of STZ (55 mg/kg b.w. in 0.05 M ice-cold citrate buffer solution of pH 4.5) in overnight fasted rats. However, the STZ caused fatal hypoglycemia due to massive pancreatic insulin release, so the rats were supplied with sucrose (prepared as 10% w/v water solution) for the next 24 h. In addition, the HCC was induced by a single intraperitoneal injection of the carcinogenic chemical diethylnitrosamine (DEN; 200 mg/kg b.w. prepared in 0.9% w/v NaCl solution) (Furman 2015; Das et al. 2019a).

Experimental protocol

The rats were randomly divided into five groups, with six in each group (n = 6), as given in Table 1. Following 18 weeks of study, the blood/serum was collected from the overnight fasted rats after anesthetization (under mild ether anesthesia) for carrying out the biochemical and NMR analysis. Later, the liver tissues were collected from the euthanized rats for carrying out the histopathological findings, and finally, the carcasses were disposed of by incineration.

Table 1 Experimental design and treatment protocol (for a duration of 18 weeks)

Estimation of glucose and lipid profile levels

The study of serum fasting glucose and lipid profile levels such as triglycerides (TG), total cholesterol (TC), and high-density lipoprotein cholesterol (HDL-c) were determined according to the procedure given by the commercial kits (Erba Transasia Bio-Medicals Ltd., India) using Erba Chem 7 semi-automated analyzer (Friedewald et al. 1972).

Preparation of serum samples and acquisition of ¹H-NMR spectra

The ¹H-NMR spectra for all serum samples were obtained on the JEOL advanced ECZ 400S spectrometer operating at 400 MHz proton frequency using a 5-mm TH5 direct broadband probe. Briefly, 300 µL of serum and 200 µL of deuterium oxide (D₂O) was taken in a cleaned dried 5-mm NMR tube. A reusable sealed capillary tube containing 30 µL of 0.375% of the sodium salt of trimethylsilyl 2,2,3,3-tetradeuteropropionic acid (TSP) in D₂O was inserted into the NMR tube before recording the spectra. The TSP served as a chemical shift reference and an external standard for quantitative estimation, whereas D₂O served as the field-frequency lock. Further, a one-dimensional Carr-Purcell Meiboom-Gill (CPMG) pulse sequence acquired ¹H-NMR spectra. The CPMG eliminates broad signals from protein, lipids, and macromolecules, and significantly altered metabolites were identified and discussed. All the spectra were recorded with 16-time domain data points, 15 ppm spectral width, 256 number of scans, 4 dummy scans, relaxation delay of 5 s, constant receiver gain value of 60, and tau interval of 0.172 ms. The NMR software version delta 5.0.5.1. was used for phase and baseline correction and for getting integral (area under the curve) value.

Quantification of the metabolites

The concentration of the metabolites was determined using integral values of the unambiguous signals for a known concentration of TSP, which was taken in a capillary tube and been calculated using the following formula:

$${\text{Cmetabolite}}\left( {{\text{mmol}}} \right) = \left( {Ax \times Mx \times {\text{Nr}} \times {{{\text{Wr}}} \mathord{\left/ {\vphantom {{{\text{Wr}}} {{\text{Ar}}}}} \right. \kern-\nulldelimiterspace} {{\text{Ar}}}} \times {\text{Mr}} \times {\text{Nx}} \times V} \right) \times 1000$$

where Ax and Nx are the integral area and number of protons of the metabolites; Mr and Mx are the molecular weight of TSP and metabolite; Wr is the weight of TSP taken in the capillary tube; Ar represents an integral area of TSP; and Nr represents the number of protons of TSP which serves as an external reference calibrated at 0.0 ppm for assignment of metabolites.

Histopathological findings of the liver (H and E staining)

The formalin-fixed liver tissues were dehydrated with 60 to 100% isopropyl alcohol solution and fixed in paraffin wax. Liver section slides stained with hematoxylin–eosin (H&E) were captured with a light microscope (Olympus Microsystem, Model-CKX41, Japan).

Statistical analysis

The obtained data from the various experimental groups were expressed as mean ± SEM using the statistical software package 'GraphPad Prism version 6.0.' The results were analyzed using a one-way ANOVA test. Further, Bonferroni multiple comparison tests were followed, considering p values of ˂ 0.05 as significant.

Results

Serum biochemical parameters

The effect of asarone and metformin on serum glucose and lipid profile levels is illustrated in Table 2. The STZ- and STZ + DEN-induced rats showed significant elevation (p < 0.001) of serum glucose and lipid profile levels (TG, TC, and LDL-c) along with a decrease (p < 0.001) in HDL-c compared to the normal control group. Further, the treated groups significantly controlled the glucose and lipid profile levels compared to the STZ + DEN group. However, the variation of LDL-c and HDL-c levels in the asarone-treated group was not statistically significant.

Table 2 Effect of asarone and metformin on various blood serum parameters

¹H-NMR spectral analysis of serum samples

Figure 1 illustrates the typical representative ¹H-NMR spectra of serum samples obtained from different experimental groups. The ¹H-NMR spectral region from 0.0 to 5.3 ppm shows strong resonances from the serum samples and permits the measurements of the number of metabolites that are not overlapped by any other metabolites. They are pyruvate, lactate, creatine, acetate, valine, alanine, and glutamine, as given in Table 3.

Fig. 1

Representative spectra of serum samples produced by 400 MHz 1D ¹H-NMR from normal rats A, STZ-induced diabetic rats B, STZ + DEN-induced diabetic HCC rats C, STZ + DEN + Asarone D, and STZ + DEN + Metformin E, respectively. STZ, streptozotocin; DEN, diethylnitrosamine

Table 3 Effect of asarone and metformin on different serum metabolite levels

Changes in metabolite concentrations

Figure 2 shows the dynamic changes of the metabolites (denoted in the green boxes) and their relevant pathways at the end of 18 weeks obtained from the KEGG and HMDB databases. To extract more details and the changes associated with the metabolic pathways with the progression of the disease, serum NMR spectra of metabolites were quantified (Table 3). The STZ-treated rats alone have significant metabolic modifications compared with the normal rats, including (1) decreased levels of pyruvate, alanine, and glutamine, although reduced levels of lactate, creatine, and valine were observed, but not statistically significant and; (2) increased levels of acetate. Further, the diabetic HCC (STZ + DEN) group reveals several metabolic alterations compared with the normal rats, (1) increased levels of pyruvate, lactate, creatine, and acetate; and (2) decreased levels of alanine and glutamine, with no significant alteration in the levels of valine. Following treatment with asarone and metformin in the diabetic HCC group, a substantial alteration was observed in the levels of metabolites compared with the STZ + DEN rats, (1) decreased levels of pyruvate, lactate, creatine, and acetate; (2) increased levels of alanine and glutamine, except no changes for creatine in metformin-treated, alanine in asarone-treated and valine for both the treatment groups.

Fig. 2

The schematic metabolic pathways of hepatocarcinogenesis during diabetic conditions obtained from the KEGG and HMDB databases. The dynamic changes of the metabolites (denoted in the green boxes) detected by ¹H-NMR serum analysis display the link with the metabolic pathways (represented in the red dotted boxes). KEGG, Kyoto Encyclopedia of Genes and Genomes (https://www.genome.jp/kegg/pathway.html); HMDB, Human Metabolome Database (https://hmdb.ca/metabolites)

Histopathological features

The typical histopathological features of rat livers for all the groups by hematoxylin–eosin (H and E) staining are represented in Fig. 3. The STZ-treated liver at the end of 18 weeks showed the features of congestion in the central vein (CV), portal triad (PT), and sinusoids (S) with severe inflammation in between the hepatocytes compared to normal livers where the hepatic lobules remain intact. In contrast, the structural disorganization in the livers of STZ + DEN rats could be observed with the development of all the characteristic features of HCC. Following treatment with asarone and metformin, the extent of the toxic effect was reduced compared to STZ + DEN rats.

Fig. 3

Representative histological features of liver sections stained with hematoxylin–eosin (H and E) obtained from normal rats a, STZ-induced diabetic rats b, STZ + DEN-induced diabetic HCC rats c, STZ + DEN + Asarone d, and STZ + DEN + Metformin e, respectively. Black arrowheads indicate congestion in CV and PS. (a, b, d, e, magnification: 200; c, magnification: 100). STZ, streptozotocin; DEN, diethylnitrosamine; CV, central vein; PS, portal system

Discussion

Evidence in both experimental and clinical-based studies suggested a link between diabetes and cancer (Das and Gadad 2021; Das et al. 2021; Sciacca et al. 2013; Szablewski 2014). There is a higher risk of the prevalence of pancreatic, liver, colorectal, kidney, and endometrial cancers in persons with diabetes (Rahman et al. 2021). Hepatocellular carcinoma (HCC) is a multistep process involving genetic, DNA changes and alterations in metabolites throughout the disease’s initiation, promotion, and development (Hlady and Robertson 2018). Although numerous studies have elucidated the systemic abnormalities in several metabolites involving the difference between HCC and normal tissues (Wang et al. 2011; Tan et al. 2012), little is known about the characteristic metabolomic changes concerned with the progression of HCC during diabetic conditions. Henceforth, in this study, the ¹H-NMR-based metabolomics was used to characterize the metabolic changes in experimentally induced diabetic HCC conditions. Furthermore, we evaluated the effect of asarone and metformin with the changes in the key metabolites in controlling the progression of the disease.

It is a well-known fact that the liver plays an essential role in maintaining the homeostasis of glucose, lipid, and energy metabolism. Dysregulation of this system is a significant aspect of the development of diabetes. The STZ selectively targets and destroys the insulin-secreting beta-cells of the pancreas leading to degressive glucose uptake and utilization and resulting in hyperglycemia (Hatting et al. 2018; Szkudelski 2001). The metabolites related to glucose metabolism pathways, such as pyruvate and lactate, are involved in the development of diabetes. In the NMR spectra of diabetic rats, we found lower levels of pyruvate and lactate, which is consistent with earlier findings (Zhao et al. 2010; Amaral et al. 2006). This decline in pyruvate and lactate levels suggests that the glycolysis pathway is impaired and may cause less production of ATP, which is involved in the synthesis of energy needed for the body. Further, the progression of hepato-carcinoma has also been linked with the alteration in plasma lipid and lipoprotein metabolism. As a source of energy and support for cell division and fatty acid derivatives, the tumor cells are highly dependent on the metabolism of the lipids, thereby directly linking to cell survival and growth (Beloribi et al. 2016). In this study, the STZ- and STZ + DEN-treated rats showed significantly altered lipid profile levels as TC, TG, LDL-c, and HDL-c. During diabetes, STZ-induced animals rely on other fuels for energy, such as lipids or free fatty acids, due to impaired glucose metabolism. This suggests the breakdown of lipids and further mobilization of free fatty acid from the peripheral fat repository. Henceforth, this results in triglycerides, cholesterol, and other lipids, as indicated in the study. Our results agree with a previous study that suggests that the rise in the acetate level is due to an increase in the beta-oxidation pathway of fatty acids during the diabetic condition (Chengfeng et al. 2014). Moreover, consistent with previous studies, the levels of amino acids were also reduced, indicating enhancement of gluconeogenesis during the diabetic state (Wijekoon et al. 2004; Atherton et al. 2006). These amino acid concentration alterations are comparable to those reported in type 1 diabetes. This implies that the changed glutamine and alanine levels in diabetic rats indicate the maintenance of fasting hyperglycemia (Chengfeng et al. 2014).

The aberrant glucose, lipid, and amino acid metabolites throughout the progression of hepatocarcinogenesis induced by DEN during STZ-induced diabetic conditions confirmed that energy supply is vital for tumor cells (Boroughs and Deberardinis 2015). As observed, the energy supply provided via the glycolysis pathway yields a more considerable amount of pyruvate and lactate during the diabetic HCC condition. This indicates an increase in energy demand through glycolysis during the process of hepatocarcinogenesis and is considered one of the hallmarks of cancer (Pavlova and Thompson 2016). Creatine is another metabolite participating in the energy metabolism for the maintenance of fluctuating energy demands. In our study, elevated creatine levels were observed compared to normal rats, suggesting the difference related to the energy demand in diabetic HCC conditions (Barcelos et al. 2016). Further, a significant increase in the acetate content of diabetic HCC reflects the metabolism of hepatic lipids, which agrees with the serum lipid profile showing increased levels of different lipids. This directs the supplement of energy source for the proliferation of tumor cells besides glucose metabolism and could be used as a potential biomarker for diabetic HCC. Glutamine, the non-essential amino acid in cancer, is highly heterogeneous, and it depends on a variety of factors that collectively impact its role in cancer cell metabolism (Cluntun et al. 2017). The abnormal alterations in glutamine levels during diabetic HCC conditions influence its role in metabolism by the fast-proliferating hepatocytes for meeting the need for energy supply.

Furthermore, the alterations seen in the STZ + DEN-induced group compared to normal rats were validated by serum biochemical results consisting of several liver function enzymes and biomarkers as confirmed in our previous studies. This model has also been used to explore the histo-morphological signatures starting from inflammation to HCC in STZ + DEN-treated rats to replicate the clinical evidence for the diabetic HCC condition. Here, we have confirmed the induction of diabetes and HCC in rats with the help of biochemical markers such as glycosylated hemoglobin (HbA_1c), insulin, gamma-glutamyl transferase (GGT), and α-fetoprotein (AFP) (Das et al. 2019a; Das and Gadad 2020).

Epidemiological, clinical, and experimental studies suggest that metformin reduces the cancer risk with diabetes (Li et al. 2018). Our study also observed that metformin either decreased or altered the severity of hepatocarcinogenesis in diabetic conditions as confirmed by ¹H-NMR-based metabolomics and histopathological evidence. The results are based on the lipid profile Furthermore, the test compound asarone also attenuated the characteristic metabolic changes in the pathological process of diabetic HCC conditions.

Conclusions

In conclusion, ¹H-NMR-based metabolomics approach was used to elucidate the small molecular metabolites in the serum samples for studying the pathological progress of diabetic HCC. We primarily outlined the peculiarity in glucose, lipid, and amino acid metabolisms over the development of hepatocarcinogenesis in rats during the diabetic condition, which provided us with a better understanding of the metabolite changes. Furthermore, asarone and metformin successfully improved the metabolic changes caused by STZ and DEN in diabetic HCC rats, indicating altered energy metabolism with metabolite changes. However, a further detailed investigation is needed to know about the possibility of involvement of pivotal metabolic pathways shared between both the diseases. Also, there is a need to identify and quantify the larger metabolites linked to diabetic HCC conditions.