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Evaluating the allelopathic potentiality of seed powder of two Brassicaceae plants in controlling Orobanche ramosa parasitizing Lycopersicon esculentum Mill. plants
Bulletin of the National Research Centre volume 43, Article number: 101 (2019)
Orobanche ramosa (Broomrape) is an obligate root parasite belonging to Orobanchaceae. It causes a great damage to tomato plants. Several attempts have been done in order to control this parasitic weed. So, the aim of this work is to study the allelopathic efficiency of Eruca sativa (Essp) and Sinapis alba (Sasp) seed powder in comparison to the herbicidal effect of Basamid (Dazomet) treatment in controlling Orobanche ramosa (O. ramosa) infesting Lycopersicon esculentum (tomato) as well as their effect on Lycopersicon esculentum plant growth and yield.
Two pot experiments were performed in the greenhouse of the National Research Centre, Dokki, Giza, Egypt, during two successive winter seasons of 2016/2017 and 2017/2018. Treatments were applied by incorporating E. sativa (Essp) and S. alba (Sasp) seed powder to the soil at (5, 10, 15, 30, and 45 g/kg soil) concentration and Basamid treatment at 0.2 g/pot.
The results indicated that no O. ramosa infestation on Lycopersicon esculentum appeared with all Essp and Sasp concentrations except with the lowest concentration (5 g/kg soil) that reduced O. ramosa tubercles dry weight at 100 days from transplanting (DFT) to about 48.4 and 42.0%, respectively, as compared to the infected control. Lycopersicon esculentum growth as well as its yield and yield components were significantly increased with Basamid treatment at 0.2 g/pot and all Essp and Sasp concentrations (5–45 g/kg soil) except some parameters with the lowest concentration (5 g/kg soil) of both materials used when compared with their corresponding infected control. The highest yield, which exceeds the yield of the healthy control, was obtained by using both Essp and Sasp at concentrations 45 followed by 30 g/kg.
The allelopathic efficiency of Essp and Sasp is due to the presence of allelochemicals, mainly glucosinolates and phenolic compounds which could play an important role, as a natural selective bioherbicide.
Orobanche ramosa (Broomrape) is an obligate root parasite belonging to Orobanchaceae. In Egypt, three Orobanche species (O. crenata, O. ramosa, and O. aegyptiaca) are common which cause great damage to several crops such as faba bean, tomato, peas, lentil, chick pea, and also several crops (Al-Menoufi 1994; Messiha et al. 2004, 2018; Hershenhorn et al. 2009). The extent of crop losses due to broomrape infestation depends on some factors such as the extent of infestation, crop sensitivity, and the different prevailing environmental factors (El-Desoki et al. 2003). Generally, Orobanche thrives in the hot climate of the Middle East and also in the Mediterranean area as well as Asia but also in more temperate areas such as Eastern Europe, where it is one of the most serious problems in vegetable crops (Pieterse 1979; Vouzounis and Americanos 1998). All species produce a very large number of tiny seeds which remain viable for many years, germinating only in the presence of a suitable host. A single plant of Orobanche can produce over 100,000 seeds which can survive in the field for up to 20 years (Gold et al. 1978). Hence, the control of Orobanche species is very difficult. Control has been attempted through the use of trap crops (Krishnamurthy et al. 1977), germination stimulants (Saghir 1986), soil solarization (Abu-Irmaileh 1991), rotations, selection of resistant varieties (Petzoldt and Sneyd 1986), and chemical methods including fumigation with methyl bromide, metham-sodium, and Dazomet, which directly kill the seeds in the soil (Vouzounis and Americanos 1998).
Lycopersicon esculentum Mill. (Tomato) is one of the most important vegetable crops grown in Egypt and occupies the first place among vegetable crops with regard to cultivated area as well as its production and value. Cogan and Toth (2003) reported that the decrease in the yield of tomato caused by Orobanche ramosa parasitization was estimated to be in the range from 43 to 53%. Therefore, a great attention is paid to raise its production through planting the high yielding cultivars as well as improving its agricultural practices specially weed control treatments (El-Dabaa 2008). Tomato is one of the main hosts of O. ramosa (Musselman 1980; Parker 1986).
Allelopathy is a natural process in which plants interact with other plant species through releasing allelochemicals into the environment, hence affecting the growth of each other (Rice 1984). Many higher plant species contain chemicals with an allelopathic activity in different parts (Duke et al. 2000). Under certain conditions, these allelochemicals are released into the environment, either as exudation or through decomposition of plant residues that affect the neighboring plants (Einhellig 2004). This effect may be positive or negative (Zhou et al. 2011). Allelopathy is an interference mechanism, in which live or dead plant materials release chemical substances, which inhibit or stimulate the associated plant growth (Macias et al. 2003; Cheng and Cheng 2016). Allelopathic plants interfere with nearby plants by dispersing chemicals into the soil that may inhibit plant growth, nutrient uptake, or germination (Singh et al. 2003). Allelochemicals like phenolic compounds, flavonoids, terpenoids, alkaloids, amino acids, and glucosinolates were found in different allelopathic plants (Fahey et al. 2001; Einhellig 2002; Velasco et al. 2008; Ahmed et al. 2012).
Brassicaceae family has allelopathic potential on the growth of other plants (Fenwick et al. 1983; Velasco et al. 2008; Zaji and Majd 2011; Martinez-Ballesta et al. 2013). They mainly produce glucosinolates that are not biologically active under normal conditions. When the plant tissues and cells are disrupted, they are hydrolyzed by the enzyme myrosinase, resulting in several degradation products, including isothiocyanates, nitriles, thiocyanates, epithionitriles, and oxazoliolines (Bones and Rossiter 2006). The main breakdown products are isothiocyanates which are phytotoxic (Fenwick et al. 1983; Fahey et al. 2001; Bennett et al. 2002; Kim and Ishii 2006; Zaji and Majd 2011; Martinez-Ballesta et al. 2013) and have pesticidal activities (Borek et al. 1994; Velasco et al. 2008). Brassicaceae seed plants have been reported to be higher in glucosinolate levels than the leaves, stems, and roots (Fahey et al. 2001; Velasco et al. 2008).
Therefore, the aim of the present work is to assess the allelopathic ability of the seed powder of two Brassicaceae plants, i.e., Eruca sativa and Sinapis alba, in controlling another member from the Orobanchaceae family (Orobanche ramosa) parasitizing Lycopersicon esculentum plants.
Materials and methods
Two pot experiments were carried out during two successive winter seasons of 2016/2017 and 2017/2018 in the greenhouse of National Research Centre, Dokki, Giza, Egypt. Lycopersicon esculentum (Tomato) seedlings (cultivar Super marmand) and seeds of both watercress (Eruca sativa) and white mustard (Sinapis alba) were obtained from Agricultural Research Centre, Giza, while parasitic weed seeds of Orobanche ramosa (broomrape) were obtained from the Weed Control Department, Ministry of Agric., Giza, Egypt. Clean seeds of both E. sativa and S. alba were grinded to fine powder and immediately incorporated to the soil surface before transplanting L. esculentum seedlings at concentration of 5, 10, 15, 30, and 45 g/kg soil. The experiment consisted of 13 treatments, i.e., two controls (healthy and infected), 10 treatments by different concentrations (5, 10, 15, 30, and 45 g/kg) of both (Essp) and (Sasp), and a treatment with Basamid. Each treatment is represented by nine pots (30 cm diameter) filled with 5 kg Nile clay soil. All treatments, except the healthy control were infected with O. ramosa seeds (0.2 g/pot) at 5 cm depth from the soil surface. The experiment also included herbicidal treatment with Basamid (Dazomet) for comparison with the allelopathic effect of both E. sativa and S. alba treatments. Basamid granules (Tetra hydro -3,5- dimethyl-2H-1,3,5-thiadiazine2-thione) were mixed in the soil infected with O. ramosa at the concentration 0.2 g/pot 10 days before planting the host seedlings. Three L. esculentum seedlings were sown/pot. All pots were distributed in a complete randomized design. Three replicates were collected from each treatment at 45, 80, and 100 days from transplanting. The normal cultural practices of growing L. esculentum plants were followed especially fertilization and irrigation.
In each season, three replicates were collected from each treatment at 80 and 100 days from transplanting (DFT) to determine number, length, and fresh and dry weight of O. ramosa tubercles/pot.
Lycopersicon esculentum plants
In both seasons, samples of Lycopersicon esculentum plants at 45, 80, and 100 DFT were collected from each treatment: plant height (cm), root length (cm), number of leaves/plant, number of branches/plant, fresh and dry weight of shoot/plant (g), and fresh and dry weight of root/plant (g). Also, the number and weight of fruit set/plant (g) were recorded at 80 and 100 (DFT).
Total glucosinolates (μ mol/g DW)
Total glucosinolates were extracted from dry samples of seed powder of both E. sativa and S. alba. Glucosinolates were measured by determining the liberated glucose released during hydrolysis by myrosinase enzyme (Rauchberger et al. 1979). The resulting glucose was determined colorimetrically according to the methods defined by Nasirullah and Krishnamurthy (1996).
Total phenolic contents (mg/g DW)
Total phenolic contents of both E. sativa and S. alba seeds were determined colorimetrically using Folin and Ciocalteu phenol reagent according to the method defined by Snell and Snell (1953).
All data were statistically analyzed according to Snedecor and Cochran (1980), and the treatment means were compared by using least significant difference (LSD) at 5% significant level.
Weed growth parameters
The results in Table 1 showed the potentiality of controlling Orobanche ramosa parasitizing L. esculentum by incorporating different seed powder concentrations (5–45 g/kg soil) of Eruca sativa (Essp) and Sinapis alba (Sasp) as well as Basamid treatment (0.2 g/pot), to the soil. The lowest Essp concentration (5 g/kg soil) significantly reduced O. ramosa infestation and decreased number, length, and fresh and dry weight of O. ramosa tubercles/pot at the two ages of growth (80 and 100 DFT) as compared to their corresponding infected control. Also, the same Sasp concentration (5 g/kg soil) induced significant reduction in the same O. ramosa parameters only at the second age of growth (100 DFT), since no O. ramosa infestation tubercles appeared on L. esculentum at the first age of growth (80 DFT). The rate of reduction of O. ramosa tubercles’ dry weight was recorded with the lowest concentration (5 g/kg soil) of Essp and Sasp) reached to 48.36 and 41.96%, respectively, as compared to the infected control. It is worthy to mention that no O. ramosa infestation occurred on L. esculentum plants by applying different treatments of both Essp and Sasp from (10–45 g/kg soil) concentrations as well as Basamid treatment at 0.2 g/pot.
Lycopersicon esculentum growth
The results in Tables 2, 3, and 4 show that most growth parameters of L. esculentum at 45, 80, and 100 (DFT) were significantly increased with all seed powder concentrations used (5–45 g/kg soil) of both E. sativa and S. alba and Basamid treatment at 0.2 g/pot compared to their corresponding infected controls. The highest significant increases in the different L. esculentum growth parameters were recorded with both 30 and 45 g/kg soil concentrations of Essp and Sasp as well as Basamid treatment (0.2 g/pot), especially at the later growth age (100 DFT). Treatment with 30 and 45 g/kg soil concentrations not only alleviated the harmful effect of O. ramosa parasite but also induced increases in most growth parameters of the plant. At 100 DFT, Essp treatments at 30 and 45 g/kg soil concentrations induced increases in the total dry weight of plant (shoot + root) reached to 21.10 and 35.62 %, respectively, over the corresponding healthy control, while the same treatment of Sasp achieved increases in the same plant parameter, reached to 10.05 and 31.86 %, over the corresponding healthy control.
Lycopersicon esculentum yield
The results of yield and its components of L. esculentum such as the number of fruit set/plant and weight of fruit set/plant (g) at 80 and 100 DFT recorded in Table 5 revealed that all Essp and Sasp concentrations used (5 to 45 g/kg soil) as well as Basamid treatment (0.2 g/pot) at both ages of growth significantly increased all yield parameters of L. esculentum, except the number of fruit set/plant at the lowest concentration (5 g/kg soil) used of both (Essp) and (Sasp) as compared to their corresponding infected control. The best results of L. esculentum yield were recorded with 30 and 45 g/kg soil concentrations of both Essp and Sasp as well as by 0.2 g/pot Basamid treatments. The applied treatments with the highest concentration (45 g/kg soil) of both Essp and Sasp not only alleviated the harmful effect of O. ramosa parasitizing which reached to 74.6% as shown in the weight of fruit set/plant at 100 DFT but also increased this character than the healthy control. At 100 DFT, Essp at 45 g/kg soil concentration induced increases in weight of the fruit set/plant reached to 48.16 and 77.94%, respectively, over the corresponding healthy control and Basamid treatment (0.2 g/pot), while treatment with Sasp with the same concentration recorded increases in the same yield parameter reached to 24.90 and 50.01%, respectively, over the corresponding healthy control and Basamid treatment (0.2 g/pot). It is obvious from the results that Essp treatment at 45 g/kg soil achieved an increase in the weight of fruit set/plant at 100 DFT reached to about the double of that recorded with the same treatment of Sasp in the same yield parameter (48.16: 24.90). It is worthy to mention that the natural treatments of both Essp and Sasp at 45 g/kg soil concentration induced increases exceeding those caused by the herbicide Basamid treatment in L. esculentum yield components.
Changes in total glucosinolates and total phenolic content in Eruca sativa and Sinapis alba seed powder
The results in Table 6 illustrated that total glucosinolates in Essp is higher than that in Sasp, whereas total phenolic content in Sasp is higher than that in Essp.
Our previous work at the botany department of the National Research Centre showed the allelopathic efficiency of seed powder of some Brassicaceae plants as Eruca sativa, Sinapis alba, Brassica rapa, and Raphanus sativa) in controlling some annual as well as perennial weeds (Messiha et al. 2013; Ahmed et al. 2014, 2016; El-Masry et al. 2015; El-Rokiek et al. 2017). Moreover, Messiha et al. (2018) showed the high allelopathic efficiency of the seed powder of one of the Brassicaceae plants (Sinapis alba) in controlling (O. crenata) parasitizing faba bean plants. There is no doubt that the main obstacle in controlling Orobanche infestation to several crops is the durable seed bank of the parasite in the soil, which could remain viable for decades (Gold et al. 1978). This means that as long as the parasite seeds are not controlled, the parasite will persist till a suitable host is present. Therefore, it will be an advantage if we can make use from the allelopathic potentiality of the residues of some plants or from its seed powder as a tool for controlling this parasite. In this connection, it is worthy to mention that our previous work at the National Research Centre showed that using the seed powder of Sinapis alba plants could be used as a powerful tool in decreasing the number of infested faba bean plants with Orobanche crenata; this means obviously that this practice adversely affects the viability of the seeds of the parasite and hence decreased the number of infected faba bean plants (Messiha et al. 2018). Therefore, it was thought advisable to know the possibility of getting such promising results to control O. ramosa which infects tomato plants. Moreover, the results of the present work showed also that the allelochemical potentiality of the seed powder of the two Brassicaceae plants is not only constricted in decreasing the number of infested tomato plants (as shown in the number of tubercles/pot), but also it stimulated significantly the growth and yield of the host plant when compared with both healthy control and those treated with the herbicide Basamid.
In this connection, it is worthy to mention that some previous reports showed that allelochemical which inhibit the growth of some species at certain concentration may stimulate the growth of the same or different species at different concentrations (Ahmed et al. 2012, 2014; Messiha et al. 2013, 2018; Baeshen, 2014; El-Masry et al. 2015).
Incorporating the seed powder of Eruca sativa or Sinapis alba to the soil is a powerful tool in preventing Orobanche ramosa infestation to Lycopersicon esculentum and also increased significantly its yield.
The incorporation of the seed powder of Eruca sativa or Sinapis alba have privileges when compared with the herbicide Basamid, since treatment with herbicide needs a period of about 10 to 15 days before transplanting.
Availability of data and materials
The datasets generated and/or analysed during the current study are included in this published article
Eruca sativa seed powder
Days from transplanting
- L. esculentum :
- O. ramosa :
Sinapis alba seed powder
Abu-Irmaileh BE (1991) Soil solarization controls broomrapes (Orobanche spp.) in host vegetable crops in the Jordan Valley. Weed Technology 5:575–581 https://www.jstor.org/stable/3987040
Ahmed SA, El- Rokiek KG, El- Masry RR, Messiha NK (2014) The efficiency of allelochemicals in the seed powder of Eruca sativa in controlling weeds in Pisum sativum. Middle East Journal of Agriculture Research 3(4):757–762 www.curresweb.com/mejar/mejar/2014/757-762.pdf
Ahmed SA, Messiha NK, El-Masry RR, El-Rokiek KG (2012) Allelopathic potentiality of the leaf powder of Morus alba and Vitis vinifera on the growth and propagative capacity of purple nutsedge (Cyperus rotundus L.) and maize (Zea mays L.). J. Appl. Sci. Res. 8(8):4744–4751 www.aensiweb.com/old/jasr/jasr/2012/4744-4751.pdf
Ahmed SA, Messiha NK, El-Rokiek KG, Mohamed SA, El-Masry RR (2016) The allelopathic Efficiency of two Brassicaceae plant seeds in controlling weeds associating sunflower plants. Research Journal of Pharmaceutical, Biological and Chemical Sciences 7(5):158–165 https://www.rjpbcs.com/pdf/2016_7(5)/.pdf
Al-Menoufi OA (1994) The Orobanche problem and management in Egypt. The Third International workshop on Orobanche and related Striga research. Amsterdam the Netherland Royal Tropical Institute, pp 663–671
Baeshen AA (2014) Morphological and elements constituent effects of allelopathic activity of some medicinal plants extracts on Zea mays. Int J Curr Res Aca Rev 2(4):135–145 www.ijcrar.com/vol-2-4/Areej%20Ali%20Baeshen.pdf
Bennett RN, Mellon FA, Botting NP, Eagles J, Rosa EAS, Willimson G (2002) Identification of the major glucosinolate (4-mercaptobutyl glucosinolate) in leaves of Eruca sativa L. (rocket salad). Phytochemistry 61:25–30 www.ncbi.nlm.nih.gov/pubmed/12165298
Bones AM, Rossiter JT (2006) The enzymic and chemically induced decomposition of glucosinolates. Phytochemistry 67:1053–1067 www.ncbi.nlm.nih.gov/pubmed/16624350
Borek V, Morra MJ, Brown PD, McCaffrey JP (1994) Allelochemicals produced during sinigrin decomposition in soil. J Agric Food Chem 42:1030–1034 https://doi.org/10.1021/jf00040a037
Cheng F, Cheng Z (2016) Research progress on the use of plant allelopathy in agriculture and the physiological and ecological mechanisms of allelopathy. Frontiers in Plant Science 7 (1697) 1–16. www.ncbi.nlm.nih.gov/pmc/articles/PMC4647110/
Cogan L, Toth P (2003) A decrease in tomato plant caused by branched broomraps (Orobanche ramosa) parasitization. Acta - Phytotechnica 6(3):65–68
Duke SO, Dayan FE, Romagni JG, Rimando AM (2000) Natural products as sources of herbicides: current status and future trends. Weed Res 10:99–111 https://doi.org/10.1046/j.1365-3180.2000.00161.x
Einhellig FA (2002) The physiology of allelochemical action: clues and views. In: Reigosa MJ, Pedrol N (eds) Allelopathy, from Molecules to Ecosystems. Science Publishers, Enfield, pp 1–23
Einhellig FA (2004) Mode of allelochemical action of phenolic compounds. In: Macıas FA, Galindo JCG, Molinillo JMG, Cutler HG (eds) Allelopathy, chemistry and mode of action of allelochemicals. CRC Press, BocaRaton, pp 217–239
El-Dabaa MA (2008) Integration of cultural; biological and biochemical approaches in Tomato. Ph.D. Thesis, Faculty of Agriculture Ain Shams University, Egypt
El-Desoki ER, Messiha NK, El-Masry RR (2003) Sensitivity of some faba bean (Vicia faba L.) cultivars to Orobanche crenata infection. Egypt J Appl Sci 18(7):101–113
El-Masry RR, Messiha NK, El-Rokiek KG, Ahmed SA, Mohamed SA (2015) The allelopathic effect of Eruca sativa. Seed powder on growth and yield of Phaseolus vulgaris and associated weeds. Current Sci Intern (4):485–490 www.curresweb.com/csi/csi/2015/485-490.pdf
El-Rokiek KG, Ahmed SAA, Messiha NK, Ahmed SA, El-Masry RR (2017) Controlling the grassy weed Avena fatua associating wheat plants with the seed powder of two brassicaceae plants Brassica rapa and Sinapis alba. Middle East Journal of Agriculture Research 6(4):1014–1020 www.curresweb.com/mejar/mejar/2017/1014-1020.pdf
Fahey JW, Zalcmann AT, Talalay P (2001) The chemical diversity and distribution of glucosinolates and isothiocyanates among plants. Photochemistry 56:5–51 https://www.ncbi.nlm.nih.gov/pubmed/11198818
Fenwick GR, Griffiths NM, Heaney RK (1983) Bitterness in brussels sprouts (Brassica oleracea L. var. gemmifera): the role of glucosinolates and their breakdown products. J of the Sci of Food and Agric 34:73–80
Gold AH, Duanfola T, Wilhelm S, Sagen J, Chun D (1978) Condition affecting germination of Orobanche ramosa L. Proceedings of the 3rd International Congress of Pathology, Munich, p 190
Hershenhorn J, Eizenberg H, Dor E, Kapulnik Y, Goldwasser Y (2009) Phelipanche aegyptiaca management in tomato. Weed Research. 49:34–47 https://doi.org/10.1111/j.1365-3180.2009.00739.x
Kim SJ, Ishii G (2006) Glucosinolate profiles in the seeds, leaves and roots of rocket salad (Eruca sativa Mill.) and anti-oxidative activities of intact plant powder and purified 4-methoxyglucobrassicin. Soil Sci. and Plant Nutrition 52:394–400 https://doi.org/10.1111/j.1747-0765.2006.00049.x
Krishnamurthy GVG, Lal R, Nagarajan K (1977) Further studies on the effect of various crops on the germination of Orobanche seed. PANS 23:206–208
Macias FA, Marin D, Oliveros-Bastidas A, Varela RM, Simonet AM, Carrera C, Molinillo JM (2003) Allelopathy as a new strategy for sustainable ecosystems development. Biological Sciences in Space 17(1):18–23 https://www.ncbi.nlm.nih.gov/pubmed/12897457
Martinez-Ballesta M, Moreno DA, Carvajal M (2013) The physiological importance of glucosinolates on plant response to abiotic stress in Brassica. Int J Mol Sci 14:11607–11625 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709749/
Messiha NK, Ahmed SA, El-Rokiek KG, Dawood MG, El-Masry RR (2013) The physiological influence of allelochemicals in two Brassicaceae plant seeds on the growth and propagative capacity of Cyperus rotundus and Zea mays L. World Appl. Sci. J. 26 (9): 1142–1149. www.idosi.org/wasj/wasj26(9)13/2.pdf
Messiha NK, El-Dabaa MAT, El-Masry RR, Ahmed SAA (2018) The allelopathic influence of Sinapis alba seed powder (white mustard) on the growth and yield of Vicia faba (faba bean)with Orobanche crenata (broomrape). Middle East Journal of Applied Sciences 8(2):418–425 www.curresweb.com/mejas/mejas/2018/418-425.pdf
Messiha NK, Sharara FA, Elgayar SH (2004) Effect of glyphosate, fosamine ammonium and their mixture for controlling Orobanche crenata in pea (Pisum sativum L.). J Agric Sci Mansoura Univ 29(7):3979–3991
Musselman LJ (1980) The biology of Striga, Orobanche and the other parasitic weeds. Annual Review of Phytopathology 18:463–469
Nasirullah KMN (1996) A method for estimating glucosinolates in mustard/rape seeds and cake. J. Sci. Technol. 33(6):498–500
Parker C (1986) Scope of the agronomic problems caused by Orobanche species. Proceedings of a workshop on Biology and Control of Orobanche (ed. S.J. ter Borg), pp. 11–17. Wageningen
Petzoldt K, Sneyd J (1986) Orobanche Cumana control by breeding and glyphosate treatment in sunflowers. Proceedings of a workshop on Biology and Control of Orobanche (ed. S.J. ter Borg), pp. 166–171. Wageningen
Pieterse AH (1979) The broomrapes (Orobanchaceae) – a review. Tropical Agriculture 5:9–35
Rauchberger Y, Mokady S, Cogan U (1979) The effect of aqueous leaching of glucosinolates on the nutritive quality of rapeseed meal. J. Food Agric. 30:31–39
Rice EL (1984) Allelopathy, 2nd edn. Academic press, New Yourk, p 424
Saghir AR (1986) Dormancy and germination of Orobanche seeds in relation to control methods. Proceedings of a workshop on Biology and Control of Orobanche (ed. S.J. ter Borg), pp. 25–34. Wageningen
Singh HP, Batish DR, Kaur S, Kohli RK (2003) Phytotoxic interference of Ageratum conyzoideswith wheat (Triticum aestivum). Journal of Agronomy and Crop Science 189(5):341–346 https://onlinelibrary.wiley.com/doi/full/10.1046/j.1439-037X.2003.00054.x
Snedecor GW, Cochran WG (1980) Statistical Methods, 7th edn. The Iowa State Uni PRESS, Ames, p 507
Snell FD, Snell CT (1953) Colorimetric methods. Pp.:66 Volume 111. Organi, D. Van Nostrand Company, Inc, Toronto, New York, London
Velasco P, Soengas P, Vilar M, Cartea ME (2008) Comparison of glucosinolate profiles in leaf and seed tissues of different Brassica napus crops. J Amer Soc Hort Sci 133(4):551–558 http://journal.ashspublications.org/content/133/4/551.full
Vouzounis NA, Americanos PG (1998) Control of Orobanche (broomrape) in tomato and eggplant. Technical Bulletin, vol 196, pp 1–7
Zaji B, Majd A (2011) Allelopathic potential of canola (Brassica napus L.) residues on weed suppression and yield response of maize (Zea mays L.). International Conference on Chemical, Ecology and Environmental Sciences IICCEES, 2011) Pattaya, December: 457–460. http://psrcentre.org/images/extraimages/14.%201211472.pdf
Zhou Y, Wang Y, LI J, Xue YJ (2011) Allelopathy of garlic root exudates. Yingyong Shengtai Xuebao. 22(5):1368–1372 https://www.ncbi.nlm.nih.gov/pubmed/21812318
Thanks are due to the National Research Centre for use the greenhouse and laboratory facilities
This research was supported and funded by National Research Centre through the project titled: (Some strategies for improve weed control efficiency in some export crops). Project No. (11040202), during in-house projects strategy 2016-2019.
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El-Masry, R.R., El-Desoki, E.R., El-Dabaa, M.A.T. et al. Evaluating the allelopathic potentiality of seed powder of two Brassicaceae plants in controlling Orobanche ramosa parasitizing Lycopersicon esculentum Mill. plants. Bull Natl Res Cent 43, 101 (2019). https://doi.org/10.1186/s42269-019-0144-4
- Eruca sativa
- Sinapis alba
- Phenolic content
- Lycopersicon esculentum
- Orobanche ramose