Sequence analysis of TLR4 gene in river buffalo (Egyptian breed) and SNPs association with Mastitis

Bulletin of the National Research Centre

Table 2 TLR4 SNPs in healthy and mastitis river buffalo, Egyptian breed

SNP position		Amino acids variation	Genotype frequency (%)			Corrected P value (Bonferroni)
c.1-g.150		–	CC	CG	GG	0.6103
	Healthy		54	39	7
	Diseased		47	43	10
c.1-g.149		–	AA	AG	GG	1.000
	Healthy		37	48	15
	Diseased		37	48	15
c.1-g.27^a		–	CC	CA	AA	–
	Healthy		100	0	0
	Diseased		69	28	3
c.44		^Thr 15 ^Met	CC	CT	TT	0.0658
	Healthy		37	48	15
	Diseased		60	35	5
c.66		^Arg 22 ^Ser	AA	AT	TT	0.0658
	Healthy		37	48	15
	Diseased		60	35	5
c.87^a		^Cys29^stop/	CC	CA	AA	–
	Healthy		100	-	-
	Diseased		51	41	8
c.89		^Val 30 ^Gly	TT	TG	GG	0.07233
	Healthy		86	12	2
	Diseased		67	29	4
c.90			AA	AG	GG	0.07233
	Healthy		86	12	2
	Diseased		67	29	4
c.94-g.41		–	TT	TG	GG	1.000
	Healthy		30	50	20
	Diseased		30	50	20
c.572		^Ser 191 ^Tyr	CC	CA	AA	0.08152
	Healthy		33	49	18
	Diseased		17	49	34
c.575		^His 192 ^Arg/Gln	AA	AG	GG	0.006156*
	Healthy		60	35	5
	Diseased		34	49	17
c.576			TT	TG	GG	0.001653*
	Healthy		40	47	13
	Diseased		17	49	34
c.577		^Lys 193 ^Glu	AA	AG	GG	0.14995
	Healthy		27	50	23
	Diseased		17	49	34
c.579			GG	GA	AA	0.14995
	Healthy		27	50	23
	Diseased		17	49	34
c.647		^Gly216^Asp	GG	GA	AA	0.14995
	Healthy		27	50	23
	Diseased		17	49	34
c.662		^Gly 221 ^Asp	GG	AG	AA	0.09156
	Healthy		30	49	21
	Diseased		17	49	34
c.672		^Lys 224 ^Asn	AA	AC	CC	0.09156
	Healthy		30	49	21
	Diseased		17	49	34
c.812		^Ile 271 ^Arg	TT	TG	GG	0.09156
	Healthy		30	49	21
	Diseased		17	49	34
c.816		^leu 272 ^Phe	CC	CA	AA	0.09156
	Healthy		30	49	21
	Diseased		17	49	34
c.2464		^Gln 822 ^Lys/Pro/Thr	CC	CA	AA	0.61034
	Healthy		36	48	16
	Diseased		30	49	21
c.2465			AA	AC	AA	0.61034
	Healthy		36	48	16
	Diseased		30	49	21

SNPs positions were calculated taking the ATG start codon as position 1 based on the sequences
^* Significant SNPs: in bold. a: No analysis was performed for non-mutual SNPs (present only in mastitis buffaloes)