Table 1 Five Primers used to amplify the TLR4 gene
From: Sequence analysis of TLR4 gene in river buffalo (Egyptian breed) and SNPs association with Mastitis
Primer | 5′-sequence-3′ | Ann. temp | Target length |
|---|---|---|---|
1 | F1: GACAGCCATCTATAAGCCAAGG R1: TGTCTGTTTGCAAATGAACCT | 53 °C | 785 bp |
2 | F 2: AGAGTTGCTGGGAAGTCTGC R2: AACATTCCTCCTTGTACAGTGGT | 56 °C | 273 bp |
3 | F3: GCATTGTTATATCTGTGTGGAGACC R3: GAGATCTAGATACTGAAGGCTTGG | 55 °C | 985 bp |
4 | F4: CGAATTCTCAGGGGACGATA R4: GCTCTGCACACATCATTTGC | 54 °C | 953 bp |
5 | F5: GACTGCAGTTTCAACCGTATCA R5: TGGCAGCATTTACTTGTTAACTGA | 55 °C | 953 bp |