Precise isolation of high-quality RNA from leaves and storage roots of cassava (Manihot esculenta Crantz) for gene expression studies

Bulletin of the National Research Centre

Table 1 Primer sets of the selected reference genes used for RT-qPCR

S/N	Gene name	Forward 5′ → 3′	Tm	Length	Reverse 5′ → 3′	Tm	Length
1	Ubiquitin conjugating enzyme E2–10 (U10)	AGTCACTATTCATTTCCCGCCCGA	59.9	24	TGTCCAGACAAATGCTTCCGTTGC	59.9	24
2	Glyceraldehyde-3-phosphate dehydrogenase (G3pdh)	AAGCCAGTCACTGTTTTCGG	58.69	20	CGTACCTTCAAGTGAGCAGC	58.93	20
3	Ribosomal 18S	ATTGGAGGGCAAGTCTGGTG	59.96	20	CTTCAAAGTAACAGCGCCGG	59.83	20