Table 6 Effect of sucrose on coumarin-inducing media on development of in vitro potato micro-tuberization
Characters of microtuberization | Sucrose conc | Coumarin conc. (mg/l) | |||
|---|---|---|---|---|---|
0 | 20 | 40 | 60 | ||
No. of days to begin tuberization | 3 | 18 ± 0.577b | 18 ± 0.577b | 18 ± 0.577b | 25 ± 0.577a |
6 | 8 ± 0.577c | 8 ± 0.577c | 8 ± 0.577c | 8 ± 0.577c | |
9 | 8 ± 0.577c | 8 ± 0.577c | 8 ± 0.577c | 8 ± 0.577c | |
No. of days to reach 50% tuberization | 3 | 25 ± 3.282e | 46 ± 1.527c | 39 ± 1d | 57 ± 0.577a |
6 | 13 ± 1h | 21 ± 0.577f | 51 ± 1.527b | 27 ± 1.527e | |
9 | 18 ± 0.577fg | 15 ± 0.577gh | 27 ± 1.154e | 25 ± 0.577e | |
% of tuberization after 8 weeks | 3 | 75 ± 0.577b | 50 ± 2.886de | 66.7 ± 0.866c | 40 ± 2.886e |
6 | 100 ± 0.577a | 60 ± 2.886cd | 60 ± 2.886cd | 100 ± 0.577a | |
9 | 75 ± 1.527b | 85.7 ± 8.256b | 100 ± 0.577a | 80 ± 11.547b | |
No of tubers per plantlet after 8 w | 3 | 2.3 ± 0.440a | 1 ± 0.577c | 1 ± 0.577c | 1 ± 0.577c |
6 | 1.6 ± 0.115b | 1.7 ± 0.057abc | 1 ± 0.577c | 1.4 ± 0.115bc | |
9 | 1.3 ± 0.057bc | 1.7 ± 0.1abc | 2.1 ± 0.057abc | 1.8 ± 0.264abc | |
Weight of microtubers | 3 | 16.6 ± 0.288i | 55 ± 1.154d | 52 ± 1.154e | 6 ± 0.577j |
6 | 33.7 ± 0.317h | 33.7 ± 0.317h | 33.7 ± 0.317h | 39.7 ± 0.115f | |
9 | 63 ± 1.732c | 98.8 ± 0.461b | 35.2 ± 0.115g | 106 ± 1.154a | |