Fig. 6

Fluorescent beads in the head area of hatched chick. A Transparent treatment of the whole brain. Photographs of (a) the whole brain fixed with 4% paraformaldehyde and (b) the transparent whole brain. B Multiple beads were detected in the brains. Fluorescent beads in the transparent brain tissue were photographed; (a) a large part of the whole brain (bright field), (b) the restricted region (open rectangle in (a)) (relief contrast), and (c) the same region as in (b) (fluorescence). C The number of brains with beads among the hatched chicks. In three independent experiments (Fig. 5C), the numbers of manipulated brains with fluorescent beads were counted