Fig. 5

Manual implantation of fluorescent beads into the head area of chick embryos. A Stage-13 and -18 embryos. Chick embryos at stages 13 (a) and 18 (b), taken from the textbook (Bellairs and Osmond 2005). B A photograph of one of the first hatched chicks after inoculation. (a) After implanting the micro-beads into the embryos, the eggshells were sealed with tape. Then the manipulated embryos were incubated until hatching. (b) The first animal to have micro-beads in its brain at birth. C The hatch rate of the inoculation experiments. Results of three independent experiments using stage-18 embryos