Horizontal transfer of antibiotic resistance genes into microflora and blood cells in rats fed on GM-diet

Bulletin of the National Research Centre

Table 4 Prevalence of transfer of nptII and aadA genes into blood cells of rats fed on GM-diet

Rats	Samples Codes and results of PCR amplification of nptII and aadA genes
	0-Day samples¹			30 Days samples²			60 Days samples³			90 Days samples⁴
	Code	nptII	aadA	Code	nptII	aadA	Code	nptII	aadA	Code	nptII	aadA
A	A0	−	−	A30	+	+	A60	+	−	A90	+	−
B	B0	−	−	B30	+	+	B60	+	−	B90	+	+
C	C0	−	−	C30	+	+	C60	+	−	C90	+	−
D	D0	−	−	D30	+	−	D60	+	+	D90	−	−
E	E0	−	−	E30	+	+	E60	+	+	E90	+	+
F	F0	−	−	F30	+	+	F60	+	+	F90	+	+
G	G0	−	−	G30	+	+	G60	+	−	G90	+	+
H	H 0	−	−	H 30	+	−	H 60	+	−	H 90	+	+
K	K0	−	−	K30	+	−	K60	+	−	K90	+	+
%^P				%^P	100	66.6	%^P	100	33.3	%^P	88.8	66.6

¹Blood samples collected from control rats just before weaning. ²Blood samples collected from rats after being fed on the GM-diet for 30 days. ³Blood samples collected from rats after being fed on the GM-diet for 60 days. ⁴Blood samples collected from rats after being fed on the GM-diet for 90 days. %^P: Percentage of transfer of GM sequences