Table 1 Detected Cytochrome P450 proteins constituting the CYPome of the prokaryotic community of 3S metagenome
CYP Family | CYP Subfamily | E-value | Taxonomic affiliation |
|---|---|---|---|
Bacillus | |||
CYP102 | CYP102A1 (BM3), CYP102A2 (cypD), CYP102A3 (cypB), CYP102A5 | CYP102A1 (7.0e−18), CYP102A2 (9.0e−18), CYP102A3 (6.0e−17), CYP102A5 (7.0e−16) | Bacillus megaterium strain ATCC 14,581/DSM 32/JCM 2506/NBRC 15,308 / NCIMB 9376/NCTC 10,342 / VKM B-512, Bacillus subtilis strain 168, Bacillus cereus strain ATCC 14,579/ DSM 31/JCM 2152/NBRC 15,305/NCIMB 9373/NRRL B-3711 |
CYP106 | CYP106A2 (MEG), CYP106A1 (BM-1) | CYP106A2 (8.0e−34), CYP106A1 (2.0e−33) | Bacillus megaterium, Bacillus megaterium strain ATCC 14,581/DSM 32/JCM 2506/NBRC 15,308/NCIMB 9376/NCTC 10,342/VKM B-512 |
CYP107 | CYP107DY1, CYP107AA1 (pksS, RubU), CYP107H1 (bioI), CYP107J1 | CYP107DY1 (1.0e−33), CYP107AA1 (6.0e−38), CYP107H1 (1.0e−28), CYP107J1 (1.0e−33) | Bacillus megaterium strain ATCC 12,872 /QMB1551, Bacillus subtilis strain 168 |
CYP109 | CYP109B1 (yjiB), CYP109A1 | CYP109B1 (8.0e−36), CYP109A1 (8.0e−31) | Bacillus subtilis strain 168, Bacillus subtilis subsp. spizizenii strain ATCC 23,059 / NRRL B-14472 /W23 |
CYP134 | CYP134A1 (CYPX) | CYP134A1 (2.0e−23) | Bacillus subtilis strain 168 |
Streptomyces | |||
CYP28 |  | CYP28 (2.0e−30) | Streptomyces avermitilis strain ATCC 31,267/ DSM 46,492/ JCM 5070/ NBRC 14,893/ NCIMB 12,804/ NRRL 8165/ MA-4680 |
CYP105 | CYP105A1, CYP105D1 (SOY), CYP105C1, CYP105A3, CYP105B1, CYP105L1(tylH1), CYP105B2, CYP105H4 (AmphN) | CYP105A1 (2.0e−25), CYP105D1 (3.0e−30), CYP105C1 (7.0e−28), CYP105A3 (2.0e−25), CYP105B1 (2.0e−24), CYP105L1 (9.0e−23), CYP105B2 (3.2e−03), CYP105H4 (7.3e−11) | Streptomyces griseolus, Streptomyces griseus, Streptomyces sp., Streptomyces carbophilus, Streptomyces fradiae, Streptomyces nodosus |
CYP107 | CYP107L1 (pikC), CYP107G1 (RapN), CYP107L14, CYP107D1 (oleP) | CYP107L1 (1.0e−33), CYP107G1 (6.3e−08), CYP107L14 (2.4e−06), CYP107D1 (8.4e−09) | Streptomyces venezuelae, Streptomyces rapamycinicus, Streptomyces virginiae, Streptomyces antibioticus |
CYP113 | CYP113B1 (tylI) | CYP113B1 (2.2e−08) | Streptomyces fradiae |
CYP129 | CYP129A2 (doxA) | CYP129A2 (2.0e−17) | Streptomyces peucetius |
CYP147 |  | CYP147 (1.4e−05) | Streptomyces sp. |
CYP151 | CYP151A (AurH) | CYP151A (5.3e−06) | Streptomyces thioluteus |
CYP158 | CYP158A1, CYP158A2 | CYP158A1 (8.0e−22), CYP158A2 (2.0e−19) | Streptomyces coelicolor strain ATCC BAA-471/ A3(2)/ M145 |
CYP161 | CYP161A3 (AmphL), CYP161C3 (penM) | CYP161A3 (1.9e−11), CYP161C3 (8.0e−29) | Streptomyces nodosus, Streptomyces exfoliatus |
CYP162 | CYP162A1 (nikQ) | 9.0e−04 | Streptomyces tendae |
CYP163 | CYP163A1 (novI), CYP163A3 (simI) | CYP163A1 (2.0e−22), CYP163A3 (2.1e−06) | Streptomyces niveus, Streptomyces antibioticus |
CYP170 | CYP170A1 (SCO5223) | CYP170A1 (1.0e−20) | Streptomyces coelicolor strain ATCC BAA-471/ A3(2)/ M145 |
CYP183 | CYP183A1 (ptlI) | CYP183A1 (3.0e−17) | Streptomyces avermitilis strain ATCC 31,267/ DSM 46,492/ JCM 5070/ NBRC 14,893/ NCIMB 12,804/ NRRL 8165/ MA-4680 |
CYP185 | CYP185A1 | CYP185A1 (7.5e−03) | Streptomyces tubercidicus |
CYP244 | CYP244A1 (staN) | CYP244A1 (1.5e−09) | Streptomyces sp. TP-A0274 |
CYP245 | CYP245A1 (staP) | CYP245A1 (2.1e−10) | Streptomyces sp. tp-a0274 |
nlmB, scnG, ncsB3, aziB1 |  | nlmB (7.7e−08), scnG (9.4e−03), ncsB3 (2.0e−28, aziB1 (2.0e−20) | Streptomyces nanchangensis, Streptomyces chattanoogensis, Streptomyces carzinostaticus, Streptomyces sahachiroi |
Mycobacterium | |||
CYP51 |  | CYP51 (1.0e−18) | Mycobacterium tuberculosis strain ATCC 25,618/H37Rv |
CYP105 | CYP105Q4 | CYP105Q4 (4.7e−03) | Mycobacterium sp. |
CYP108 | CYP108B4 | CYP108B4 (3.7e−04) | Mycobacterium ulcerans strain Agy99 |
CYP123 | CYP123A3, CYP123B1 | CYP123 (4.0e−30), CYP123A3 (3.8e−06), CYP123B1 (2.2e−08) | Mycobacterium tuberculosis strain ATCC 25,618/ H37Rv, Mycobacterium sp. |
CYP124 | CYP124E1 | CYP124 (8.0e−28), CYP124E1 (9.3e−10) | Mycobacterium tuberculosis strain ATCC 25,618/ H37Rv, Mycobacterium sp. |
CYP125 | CYP125A6, CYP125A7 | CYP125A6 (3.2e−05), CYP125A7 (5.7e−04) | Mycobacterium marinum |
CYP126, CYP128, CYP130 |  | CYP126 (3.0e−27), CYP128 (2.0e−14), CYP130 (2.0e−25) | Mycobacterium tuberculosis strain ATCC 25,618/ H37Rv |
CYP135 | CYP135A1, CYP135B1 | CYP135A1 (3.0e−21), CYP135B1 (4.0e−15) | Mycobacterium tuberculosis strain ATCC 25,618/ H37Rv |
CYP136, CYP137, CYP138, CYP139 |  | CYP136 (3.0e−17), CYP137 (2.0e−19), CYP138 (4.0e−15), CYP139 (1.0e−17) | Mycobacterium tuberculosis strain ATCC 25,618/ H37Rv |
CYP140 | CYP140A5, CYP140A7 | CYP140 (1.0e−20), CYP140A5 (2.1e−03), CYP140A7 (1.2e−09) | Mycobacterium bovis (strain ATCC BAA-935/ AF2122/97, Mycobacterium marinum M MMAR_2768, Mycobacterium ulcerans |
CYP141, CYP142 | CYP142A3 | CYP141 (5.0e−21), CYP142 (7.0e−32), CYP142A3 (4.6e−07) | Mycobacterium tuberculosis strain CDC 1551/ Oshkosh, Mycobacterium sp. |
CYP143, CYP144 |  | CYP143 (8.0e−25), CYP144 (2.0e−14) | Mycobacterium tuberculosis strain ATCC 25,618/H37Rv |
CYP150 | CYP150A6 | CYP150A6 (4.8e−03) | Mycobacterium marinum strain ATCC BAA-535/M |
CYP187 | CYP187A4, CYP187A5 | CYP187A4 (4.7e−09), CYP187A5 (7.8e−11) | Mycobacterium spp. |
CYP226 | CYP226B1 | CYP226B1 (6.4e−07) | Mycobacterium marinum M MMAR_0272 |
CYP269 | CYP269A1 | CYP269A1 (1.4e−03) | Mycobacterium marinum M MMAR_3969 |
CYP278 | CYP278A1 | CYP278A1 (6.1e−08) | Mycobacterium spp. |
Proteobacteria | |||
CYP101 | CYP101A1 (camC; p450-cam) | CYP101A1 (9.0e−23) | Pseudomonas putida |
CYP103 (pinF1), CYP104 (pinF2) |  | CYP103 (3.0e−20), CYP104 (9.0e−18) | Rhizobium radiobacter |
CYP108 | CYP108A1 (p450-terp) | CYP108A1 (1.0e−19) | Pseudomonas sp. |
CYP109 | CYP109C1, CYP109C2, CYP109D1 | CYP109C1 (2.1e−12), CYP109C2 (1.6e−15), CYP109D1 (6.0e−07) | Sorangium cellulosum So ce56 |
CYP111 | CYP111A2 (linC) | CYP111A2 (6.0e−23) | Pseudomonas putida |
CYP112 (BJ-1) | CYP112A2 (BJ-1) | CYP112A2 (2.0e−25) | Sinorhizobium fredii strain NBRC 101,917/NGR234 |
CYP114 (BJ-3) |  | CYP114 (3.0e−27) | Bradyrhizobium diazoefficiens strain JCM 10,833/IAM 13,628/NBRC 14,792/USDA 110 |
CYP116 (thcB) |  | CYP116 (1.4e−03) | Halomonas chromatireducens |
CYP127 | CYP127A1 | CYP127A1 (2.0e−23) | Sinorhizobium fredii strain NBRC 101,917/NGR234 |
CYP133 | CYP133B1, CYP133B2 | CYP133B1 (8.0e−31), CYP133B2 (1.0e−31) | Xylella fastidiosa strain Temecula1/ATCC 700,964 |
CYP167 | CYP167A1 (epoK) | CYP167A1 (4.0e−24) | Sorangium cellulosum |
CYP176 | CYP176A1 (cinA) | CYP176A1 (4.0e−24) | Citrobacter braakii |
CYP199 | CYP199A2 | CYP199A2 (1.7e−10) | Rhodopseudomonas palustris strain ATCC BAA-98 |
CYP265 | CYP265A1 | CYP265A1 (2.0e−06) | Sorangium cellulosum strain So ce56 |
CYP266 | CYP266A1 | CYP266A1 (4.9e−07) | Sorangium cellulosum strain So ce56 |
CYP267 | CYP267A1 | CYP267A1 (9.3e−05) | Sorangium cellulosum strain So ce56 |
Others | |||
Actinobacteria | |||
CYP105 | CYP105L2 (mycCI), CYP105E1 (FAS1), CYP105W1 (calE10) | CYP105L2 (5.0e−26), CYP105E1 (6.0e−28), CYP105W1 (7.7e−08) | Micromonospora griseorubida, Rhodococcus fascians, Micromonospora echinospora |
CYP107 | CYP107B1, CYP107E1 (mycCG), CYP107A1 (eryF), CYP107BR1 (vdh) | CYP107B1 (8.0e−40), CYP107E1 (1.0e−36), CYP107A1 (1.0e−36), CYP107BR1 (9.0e−36) | Saccharopolyspora erythraea strain ATCC 11,635/ DSM 40,517/ JCM 4748/ NBRC 13,426/ NCIMB 8594/ NRRL 2338, Micromonospora griseorubida, Pseudonocardia autotrophica |
CYP113 | CYP113A1 (eryK) | CYP113A1 (4.0e−32) | Saccharopolyspora erythraea strain ATCC 11,635/ DSM 40,517/ JCM 4748/ NBRC 13,426/ NCIMB 8594/ NRRL 2338 |
CYP125 | CYP125A | CYP125 (3.0e−30), CYP125A (1.2e−17) | Mycolicibacterium smegmatis strain ATCC 700,084/ mc(2)155, Rhodococcus jostii strain RHA1 |
CYP163 | CYP163B1 (salD) | CYP163B1 (3.4e−09) | Salinispora tropica |
CYP165 | CYP165A3 (oxyA), CYP165B3 (oxyB), CYP165C4 (oxyC) | CYP165A3 (3.0e−27), CYP165B3 (8.0e−25), CYP165C4 (3.0e−26) | Amycolatopsis orientalis |
CYP188 | CYP188A3 | CYP188A3 (2.0e−04) | Mycolicibacter sinensis strain JDM601 |
CYP189 | CYP189A7 | CYP189A7 (3.4e−04) | Mycolicibacter sinensis strain JDM601 |
CYP190 | CYP190A3 | CYP190A3 (6.0e−05) | Mycolicibacter sinensis strain JDM601 |
CYP245 | CYP245A2 (RebP) | CYP245A2 (1.5e−07) | Lechevalieria aerocolonigenes |
CYP255 | CYP255A (gcoA) | CYP255A (1.0e−15) | Amycolatopsis sp. strain ATCC 39,116/75iv2 |
CYP256 |  | CYP256 (1.3e−06) | Rhodococcus jostii RHA1 |
ebh, nocL, pdmW |  | ebh (1.6e−04), nocL (2.9e−18), pdmW (1.4e−08) | Amycolatopsis orientalis, Nocardia uniformis, Actinomadura hibisca |
Cyanobacteria | |||
CYP110 |  | CYP110 (4.0e−19) | Nostoc sp. strain PCC 7120/SAG 25.82/ UTEX 2576 |
CYP120 | CYP120A1 | CYP120A1 (3.0e−24) | Synechocystis sp. strain PCC 6803/Kazusa |
Archaea | |||
CYP119 | CYP119A1 | CYP119A1 (2.0e−16) | Sulfolobus acidocaldarius strain ATCC 33,909/ DSM 639/JCM 8929/NBRC 15,157/NCIMB 11,770 |