In vitro propagation and improving accumulation of coumarin in Lycium barbarum, a rare plant in the flora of Egypt

Bulletin of the National Research Centre

Table 4 Effect of TDZ and Zeatin on adventitious buds formation frequency and number of buds/callus culture

Growth regulators conc. (µM)		Mean number of adventitious buds/explant	Frequency of adventitious buds
Control		0.1^e	1ⁱ
TDZ	22.02	0.1^e	1ⁱ
	44.05	1.0^d	15^g
	88.10	0.1^e	26^d
	132.15	2.0^c	30^c
	176.20	4.0^a	50^a
	220.25	3.0^b	40^b
	264.30	0.1^e	1ⁱ
	308.35	0.1^e	1ⁱ
Zeatin	22.02	0.1^e	1ⁱ
	44.05	0.1^e	1ⁱ
	88.10	0.1^e	1ⁱ
	132.15	1.0^d	10^h
	176.20	2.0^c	25^e
	220.25	1.0^d	20^f
	264.30	0.1^e	1ⁱ
	308.35	0.1^e	1ⁱ

Means followed by the same letter are not significantly different according to Duncan’s multiple range test. The mean difference is significant at the 0.05 level. The presence of different letters in the same table indicates a high significance between the numbers. The higher significance between the results obtained, this leads to the numbers taking different letters