In vitro propagation and improving accumulation of coumarin in Lycium barbarum, a rare plant in the flora of Egypt

Bulletin of the National Research Centre

Table 3 Effects of 2,4 D and TDZ on callus formation from leaf of L. barbarum after 4 weeks of culture

Growth regulators concentration (µM)		Mean fresh weight of callus (g/jar)	Frequency of callus induction
Control		0.1ⁿ	1^l
2,4–D	55.26	0.5^m	5^k
	110.52	1.0^l	10^j
	221.04	2.5ⁱ	50^f
	331.56	4.0^g	65^e
	442.08	7.0^e	80^d
	552.60	8.7^b	93^b
	663.12	7.9^d	90^c
TDZ	55.26	1.7^k	20ⁱ
	110.52	2.4^j	40^h
	221.04	3.3^h	100^a
	331.56	5.0^f	100^a
	442.08	8.4^c	100^a
	552.60	9.5^a	90^c
	663.12	4.0^g	45^g

Means followed by the same letter are not significantly different according to Duncan’s multiple range test. The mean difference is significant at the 0.05 level. The presence of different letters in the same table indicates a high significance between the numbers. The higher significance between the results obtained, this leads to the numbers taking different letters