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Table 5 Purification steps of crude chitinase produced from Streptomyces albus

From: Antifungal activity of chitinase produced by Streptomyces species isolated from grassland soils in Futa Area, Akure

Steps Vol Chittinase activity µmol/min/ml Protein conc. mg/ml Total chittinase activity µmol/min Total protein mg Specific activity µmol/min/mg Yield % Purification fold
Crude extract 150 28.443 19.228 4266.467 221.894 1.479 100 1
Ammonium ppt 40 73.753 36.524 2950.1 80.772 2.019 69.146 1.365
Ion exch 26 95.210 42.747 2475.449 57.910 2.227 58.021 1.506
Gel filtration 16 11.876 13.991 190.02 13.581 0.848 4.454 0.574