Table 5 Purification steps of crude chitinase produced from Streptomyces albus
Steps | Vol | Chittinase activity µmol/min/ml | Protein conc. mg/ml | Total chittinase activity µmol/min | Total protein mg | Specific activity µmol/min/mg | Yield % | Purification fold |
|---|---|---|---|---|---|---|---|---|
Crude extract | 150 | 28.443 | 19.228 | 4266.467 | 221.894 | 1.479 | 100 | 1 |
Ammonium ppt | 40 | 73.753 | 36.524 | 2950.1 | 80.772 | 2.019 | 69.146 | 1.365 |
Ion exch | 26 | 95.210 | 42.747 | 2475.449 | 57.910 | 2.227 | 58.021 | 1.506 |
Gel filtration | 16 | 11.876 | 13.991 | 190.02 | 13.581 | 0.848 | 4.454 | 0.574 |