A new aspect on the correlation of ten SNPs in MIR and their target genes in dopaminergic pathways in schizophrenia

Bulletin of the National Research Centre

Table 1 The primers, their sequences, Tm, product size used for PCR

Region/SNP	Primer sequence		Tm (°C)	Product size (bp)
3′UTR NRG1/rs369770942	Forward	GGAGTATGAAACGACCCAAGAG	58/4	800	DdeI
3′UTR NRG1/rs369770942		GAGGCATATCTGGATGGATGTG	58/3
miR-125a-3P/rs143525573	Forward	TTCTAGGTCTCTGCCCCTCC	56	534	DdeI
miR-125a-3P/rs143525573	Reverse	GAGGCGCTCAGAGTAGGTTG	58
3′UTR DRD2	Forward	CTTGAATGCCAAGCACAGAA	49	249	DdeI
rs200982455	Reverse	CTCCTGTTTCCCTTCCCTTC	53
MIR326 rs530404895 rs753764536	Forward	CCTGAGCACATGGACACATT	51	239	–
rs374732351	Reverse	GGCAAGAGAAAGACAGACAGA	54
3′UTR COMT rs4680	Forward	CTCATCACCATCGAGATCAACC	61	383	NLaIII
3′UTR COMT rs4680	Reverse	GCCATCTTTACACACCCATACA	59
3′UTR COMT	Forward	CTCATCACCATCGAGATCAACC	59	160	–
rs165599	Reverse	GCCATCTTTACACACCCATACA	59
3′UTR RGS4	Forward	CTCAGAGTTCACTGGCACA	60	589	–
rs10759	Reverse	TGCAGGTTTTCTAATTGTACCC	58
MIR124	Forward	CCCTCTGCGTGTTCACAG	62	202	AseI
rs340597269	Reverse	GCATTGTTCGCCGGATTTG	62