Fig. 3

Nitric oxide and L-arginase inhibitory activities of corn silk–lemon and commercial tea. A Nitric oxide inhibitory activity as described by Balakrishnan et al. (2009). The absorbance of the solution prepared was measured at 546 nm with a spectraMax plus UV–Vis microplate reader (Molecular Devices, GA, USA). The percentage scavenging was calculated and plotted against five samples extract concentration (ug/ml). B Evaluation of L-arginase. Arginase activity was determined by the measurement of urea produced by the reaction of Ehrlich’s reagent. The optical density reading was taken at 450 nm. The control experiment was performed without the test sample and the arginase inhibitory activity was expressed as percentage inhibition and plotted against five samples extract concentration (ug/ml). (Kaysen and Strecker 1973). A: 100% CSF; B: 95% CSF and 5% LF; F: top tea; L: lipton tea; G: green tea; CSF: corn silk flour; LF: lemon flour;