Exploratory and confirmatory molecular approaches to determine genetically modified status in different crops

Bulletin of the National Research Centre

Table 5 Measurement Uncertainty using the value of within-laboratory measurement variation

Sample number	Calculated concentration %			Sr	\(\varvec{u}_{{\textbf{m}}} = \frac{{{\textbf{Sr}}}}{\sqrt{\varvec{n}} }\)	u_∆ = \(\sqrt {{\textbf{u}}_{{\textbf{m}}}^{2} + {\textbf{u}}_{{{\textbf{CRM}}}}^{\textbf{2}} }\)	U_∆ = k · u_∆	∆_m \(= \left\| {{\textbf{C}}_{{\textbf{m}}} - {\textbf{C}}_{{{\textbf{CRM}}}} } \right\|\)	Estimated enforcement level^a
Sample number	R (1)	R (2)	Mean% (C_m)	Sr			U_∆ = k · u_∆		C_m − U_∆
3	0.2385	0.1545	0.1965	0.059397	0.0424	1.5255	3.051	0.7035	– 2.855
6	1.2499	0.9262	1.08805	0.22889	0.163	1.533	3.067	0.18805	– 1.979
7	0.1492	0.1959	0.17255	0.033022	0.024	1.525	3.05	0.72745	– 2.877
8	0.1477	0.1275	0.1376	0.014284	0.01	1.525	3.05	0.7624	– 2.912
9	3.1444	4.2174	3.6809	0.758726	0.542	1.618	3.237	2.789	0.444
12	0.0807	0.0888	0.08475	0.005728	0.004	1.525	3.049	0.81525	– 2.964
13	1.937	1.903	1.92	0.024042	0.017	1.525	3.05	1.02	– 1.13
16	0.2349	0.6506	0.44275	0.293944	0.20996	1.539	3.078	0.2494	– 2.635
19	1.481	1.393	1.437	0.062225	0.045	1.526	3.051	0.537	– 1.614
22	0.2304	0.2469	0.23865	0.011667	0.008	1.525	3.05	0.66135	– 2.811
23	0.4035	0.2364	0.31995	0.118158	0.008	1.527	3.055	0.58005	– 2.735
24	0.34	0.2724	0.3062	0.047800	0.03414	1.525	3.05	0.5938	– 2.744
25	0.3579	0.3737	0.3658	0.011172	0.008	1.525	3.05	0.5342	– 2.684
27	0.1533	0.1296	0.14145	0.016758	0.012	1.525	3.05	0.7704	– 2.909
30	0.1985	0.2271	0.2128	0.020223	0.0144	1.535	3.069	0.6872	– 2.856
31	0.2244	0.9168	0.5706	0.489601	0.3497	1.565	3.129	0.3294	– 2.558
32	0.7136	0.9494	0.8315	0.166736	0.119	1.529	3.059	0.0685	– 2.228
35	2.4518	3.45	2.9509	0.705834	0.50417	1.606	3.212	2.0509	– 0.261

^aMaximum concentration stipulated in EU is 0.9%, R (1) is reaction 1, R (2) is reaction 2. u_m; uncertainty of measurement results, Sr; standard deviation of repeatability, n; number of independent measurement results, u_∆; combined uncertainty of results and certified value, \(u_{{{\text{CRM}}}}^{2}\) is uncertainty of the certified value. k; coverage factor equal to 2