Table 2 Sequences (5′–3′) of adapters and primers used in adjusted AFLP for the within and between population studies and sequences (5′–3′) of primers used for the sequencing of the conserved region of EPSP. Data in boldface shows the most informative primers in terms of number of bands and polymorphisms
From: Genetic diversity and spread of glyphosate-resistant flaxleaf fleabane
Adapters | ||
|---|---|---|
MseI | MseI adapter 1 MseI adapter 2 | GACGATGAGTCCTGAG TACTCAGGACTCAT |
PstI | PstI adapter 1 PstI adapter 2 | CTCGTAGACTGCGTACATGCA TGTACGCAGTCTAC |
Pre-amplification primers | ||
PstI pre-selective primer A | PstI + A primer | GACTGCGTACATGCAG-A |
MseI pre-selective primer C | MseI + C primer | GATGAGTCCTGAGTAA-C |
Selective amplification primers | ||
PstI selective primer ATC | PstI+ATC primer | GACTGCGTACATGCAG-ATC |
MseI selective primer CAG + (TET) | MseI + CAG Fluro (TET) primer | GATGAGTCCTGAGTAA-CAG |
MseI selective primer CAC + (NED) | MseI + CAC Fluro (NED) primer | ═ -CAC |
MseI selective primer CAT + (FAM) | MseI + CAT Fluro (FAM) primer | ═ -CAT |
MseI selective primer CAA + (VIC) | MseI + CAA Fluro (VIC) primer | ═ -CAA |
MseI selective primer CC + PstI + primer AC | MseI + CC primer + PstI + AC primer | (GATGAGTCCTGAGTAA-CC+ GACTGCGTACATGCAG-AC) |
MseI selective primer CC + PstI + primer AG | MseI + CC primer + PstI + AG primer | (GATGAGTCCTGAGTAA-CC + GACTGCGTACATGCAG-AG) |
EPSPS primers | ||
Forward primer Reverse primer | Lr-EPSP_F Lr-EPSP_R | TGCAAAAAGAGCTGTAGTCGTTGGCT AGTTGTTTCAAACCGACAACTAAGTC |