Table 1 Candidate housekeeping gene and their primer sequence for qRT-PCR
Primer’s name | Primer’s sequence 5′ to 3′ | Size | Primer’s orientation |
|---|---|---|---|
M1 | 5′-TCAAAAGATCAGACACAGCCGA-3′ | 900 bp | Forward |
5′-GATACCATGCTCGCACATAGGA-3′ | Reverse | ||
M2 | 5′-AGTGGGTGTCATACTCGGTCTA-3′ | 581 bp | Forward |
5′-CAGCTGCGCATACTTTCTGATC-3′ | Reverse | ||
M28 | 5′-CGCAACAGAAAGAAGCAATGGA-3′ | 865 bp | Forward |
5′-AGCCGCCATCCTGATAATAAGG-3′ | Reverse | ||
Housekeeping gene ACT1 | 5′-CTTCCGGTAGAACTACTGGT-3′ | 520 bp (Frohloff et al., 2001) | Forward |
5′-CCTTACGGACATCGACATCA-3′ | Reverse |