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Table 2 Analysis of variance (ANOVA) for CCD

From: Statistical optimization of chemical modification of chitosan-magnetic nano-particles beads to promote Bacillus subtilis MK1 α-amylase immobilization and its application

Source

Sum of squares

Df

Mean square

F value

P value Prob > F

 

Model

1290.78

14

92.1986

27.914

< 0.0001*

Significant

W: PEI %

54.4272

1

54.4272

16.4782

0.0023*

Significant

X: PEI activation time

215.6272

1

215.6272

65.2825

< 0.0001*

Significant

Y: GA %

413.7606

1

413.7606

125.2686

< 0.0001*

Significant

Z: GA activation Time

75.645

1

75.6450

22.9020

0.0007*

Significant

WX

4.9506

1

4.9506

1.4988

0.2489

 

WY

80.5506

1

80.5506

24.3872

0.0006*

Significant

WZ

36.9056

1

36.9056

11.1734

0.0075*

Significant

XY

0.2756

1

0.2756

0.0834

0.7786

 

XZ

10.7256

1

10.7256

3.2473

0.1017

 

YZ

52.9256

1

52.9256

16.0236

0.0025*

Significant

W2

15.7487

1

15.7487

4.7680

0.0539

 

X2

139.9616

1

139.9616

42.3743

< 0.0001*

Significant

Y2

14.5075

1

14.5075

4.3922

0.0625

 

Z2

158.4097

1

158.4097

47.9595

< 0.0001*

Significant

Error

33.0299

10

3.3030

   

C. Total

1323.8096

24

    
  1. R2 = 0.975, Adj R2 = 0.940, significant (P ≤ 0.05), non-significant (P > 0.05)
  2. Df degree of freedom