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Table 1 CCD experimental and predicted values of Ch-MNP/PEI/GA/α-amylase immobilization yield

From: Statistical optimization of chemical modification of chitosan-magnetic nano-particles beads to promote Bacillus subtilis MK1 α-amylase immobilization and its application

Run W: PEI [%] X: PEI activation time [h] Y: GA [%] Z: GA activation time [h] Immobilization yield [%]
Actual Predicted
1 0 (4) 0 (3) 0 (2.5) + 1 (4) 64.2 63.2
2 + 1 (6) − 1 (2) − 1 (1) + 1 (4) 52.2 53.4
3 − 1 (2) 0 (3) 0 (2.5) 0 (3) 58 58.1
4 0 (4) 0 (3) 0 (2.5) 0 (3) 57 57.4
5 − 1 (2) − 1 (2) + 1 (4) + 1 (4) 57.7 58.1
6 0 (4) + 1 (4) 0 (2.5) 0 (3) 51.8 53.4
7 − 1 (2) + 1 (4) − 1 (1) + 1 (4) 61.2 63
8 + 1 (6) + 1 (4) − 1 (1) + 1 (4) 59.1 57.9
9 0 (4) 0 (3) + 1 (4) 0 (3) 62.2 64.5
10 − 1 (2) − 1 (2) − 1 (1) + 1 (4) 56.5 56.4
11 0 (4) − 1 (2) 0 (2.5) 0 (3) 48.2 46.5
12 + 1 (6) − 1 (2) + 1 (4) − 1 (2) 73.8 73.3
13 − 1 (2) − 1 (2) − 1 (1) − 1 (2) 51.6 52.2
14 + 1 (6) + 1 (4) − 1 (1) − 1 (2) 62.1 63
15 + 1 (6) − 1 (2) − 1 (1) − 1 (2) 54.9 55.3
16 − 1 (2) + 1 (4) + 1 (4) − 1 (2) 70.5 70.6
17 − 1 (2) − 1 (2) + 1 (4) − 1 (2) 61.2 61.2
18 0 (4) 0 (3) − 1 (1) 0 (3) 57.4 54.9
19 + 1(6) 0 (3) 0 (2.5) 0 (3) 61.8 61.6
20 0 (4) 0 (3) 0 (2.5) − 1 (2) 66.4 67.3
21 + 1 (6) + 1 (4) + 1 (4) + 1 (4) 67.3 68
22 + 1 (6) − 1 (2) + 1 (4) + 1 (4) 64.3 64.1
23 + 1 (6) + 1 (4) + 1 (4) − 1 (2) 81.6 80.5
24 − 1 (2) + 1 (4) − 1 (1) − 1 (2) 63.2 62.1
25 − 1 (2) + 1 (4) + 1 (4) + 1 (4) 65.9 64.2