Table 1 CCD experimental and predicted values of Ch-MNP/PEI/GA/α-amylase immobilization yield
Run | W: PEI [%] | X: PEI activation time [h] | Y: GA [%] | Z: GA activation time [h] | Immobilization yield [%] | |
|---|---|---|---|---|---|---|
Actual | Predicted | |||||
1 | 0 (4) | 0 (3) | 0 (2.5) | + 1 (4) | 64.2 | 63.2 |
2 | + 1 (6) | − 1 (2) | − 1 (1) | + 1 (4) | 52.2 | 53.4 |
3 | − 1 (2) | 0 (3) | 0 (2.5) | 0 (3) | 58 | 58.1 |
4 | 0 (4) | 0 (3) | 0 (2.5) | 0 (3) | 57 | 57.4 |
5 | − 1 (2) | − 1 (2) | + 1 (4) | + 1 (4) | 57.7 | 58.1 |
6 | 0 (4) | + 1 (4) | 0 (2.5) | 0 (3) | 51.8 | 53.4 |
7 | − 1 (2) | + 1 (4) | − 1 (1) | + 1 (4) | 61.2 | 63 |
8 | + 1 (6) | + 1 (4) | − 1 (1) | + 1 (4) | 59.1 | 57.9 |
9 | 0 (4) | 0 (3) | + 1 (4) | 0 (3) | 62.2 | 64.5 |
10 | − 1 (2) | − 1 (2) | − 1 (1) | + 1 (4) | 56.5 | 56.4 |
11 | 0 (4) | − 1 (2) | 0 (2.5) | 0 (3) | 48.2 | 46.5 |
12 | + 1 (6) | − 1 (2) | + 1 (4) | − 1 (2) | 73.8 | 73.3 |
13 | − 1 (2) | − 1 (2) | − 1 (1) | − 1 (2) | 51.6 | 52.2 |
14 | + 1 (6) | + 1 (4) | − 1 (1) | − 1 (2) | 62.1 | 63 |
15 | + 1 (6) | − 1 (2) | − 1 (1) | − 1 (2) | 54.9 | 55.3 |
16 | − 1 (2) | + 1 (4) | + 1 (4) | − 1 (2) | 70.5 | 70.6 |
17 | − 1 (2) | − 1 (2) | + 1 (4) | − 1 (2) | 61.2 | 61.2 |
18 | 0 (4) | 0 (3) | − 1 (1) | 0 (3) | 57.4 | 54.9 |
19 | + 1(6) | 0 (3) | 0 (2.5) | 0 (3) | 61.8 | 61.6 |
20 | 0 (4) | 0 (3) | 0 (2.5) | − 1 (2) | 66.4 | 67.3 |
21 | + 1 (6) | + 1 (4) | + 1 (4) | + 1 (4) | 67.3 | 68 |
22 | + 1 (6) | − 1 (2) | + 1 (4) | + 1 (4) | 64.3 | 64.1 |
23 | + 1 (6) | + 1 (4) | + 1 (4) | − 1 (2) | 81.6 | 80.5 |
24 | − 1 (2) | + 1 (4) | − 1 (1) | − 1 (2) | 63.2 | 62.1 |
25 | − 1 (2) | + 1 (4) | + 1 (4) | + 1 (4) | 65.9 | 64.2 |