The influence of He-Ne laser on agro-morphological criteria, ISSR marker and SDS-PAGE of Moringa oleifera

Bulletin of the National Research Centre

Table 6 SDS-PAGE of soluble proteins of two weeks old of moringa seedlings pre-treated with laser light

Number of bands	MW (KDa)	Dry seeds					Wetted seeds
Number of bands	MW (KDa)	Control	25 min	45 min	90 min	Polymorphism	Control	25 min	45 min	90 min	Polymorphism
1	185	+	+	+	+	M	+	+	+	+	M
2	155	−	+	+	+	P	-	+	+	+	P
3	120	+	−	−	−	P	+	-	–	–	P
4	115	+	+	+	+	M	+	+	+	+	M
5	112	+	+	+	+	M	+	+	+	+	M
6	106	+	+	+	+	M	+	+	+	+	M
7	102	+	+	+	+	M	+	+	+	+	M
8	93	+	+	+	+	M	+	+	+	+	M
9	82	++	++	++	++	M	++	++	++	+	M
10	79	++	++	++	++	M	++	++	++	+	M
11	76	−	+	+	+	P	-	+	+	+	P
12	70	+	+	+	+	M	+	+	+	+	M
13	65	+	+	+	+	M	+	+	+	+	M
14	59	+	+	-	-	P	+	-	–	+	P
15	57	+	+	-	-	P	+	-	+	+	P
16	52	+++	+++	+++	+++	M	+++	+++	+++	+++	M
17	49	+	+	+	+	M	+	+	+	+	M
18	47	++	++	++	++	M	++	++	++	++	M
19	45	+	+	+	+	M	+	+	+	+	M
20	44	++	++	++	++	M	++	++	++	++	M
21	43	++	++	++	++	M	++	++	++	++	M
22	41	++	++	++	++	M	++	++	++	++	M
23	39	+	+	+	+	M	+	+	+	+	M
24	38	+	+	+	+	M	+	+	+	+	M
25	37	+	+	+	+	M	+	+	+	+	M
26	36	+	+	+	+	M	+	+	+	+	M
27	35	++	++	++	++	M	++	++	++	++	M
28	34	++	++	++	++	M	++	++	++	++	M
29	31	++	++	++	++	M	++	++	++	++	M
30	29	+	+	+	+	M	+	+	+	+	M
31	28	+	+	+	+	M	+	+	+	+	M
32	27	+	+	+	+	M	+	+	+	+	M
Total number of bands		30	31	29	29	% of poly-morphism = 15.6	30	29	30	31	% of poly-morphism = 15.6
Mean of polymorphism		15.6