Table 4 The effect of different time exposure of He–Ne laser irradiation on genomic DNA of dry and wetted moringa seeds using ISSR analysis
Ser. no. | Primer | Allele size range (bp) | Total number of bands | Monomorphic bands | Polymorphic bands | Unique bands | Polymorphism percentage |
|---|---|---|---|---|---|---|---|
1 | ISSR-6 | 148–1579 bp | 24 | 14 | 10 | (232 bp) at 90 min (dry) (546 bp) at 0 min (wetted) | 41.6 |
2 | ISSR-7 | 202–1413 bp | 21 | 12 | 9 | (673 bp) at90 min (wetted) (446 bp) at 45 min (wetted) (204 bp) at 0 min (dry) | 42.9 |
3 | ISSR-8 | 168–1441 bp | 12 | 11 | 1 | 0 | 8.33 |
4 | ISSR-9 | 206–1609 bp | 17 | 13 | 4 | 0 | 23.5 |
5 | ISSR-10 | 225–1078 bp | 10 | 9 | 1 | (251 bp) at 45 min (wetted) | 10 |
6 | ISSR-11 | 188–1264 bp | 13 | 10 | 3 | (867 bp) at 0 min (dry) | 23.1 |
7 | ISSR-12 | 254–1491 bp | 17 | 3 | 14 | 0 | 82.4 |
8 | ISSR-13 | 152–1471 bp | 21 | 5 | 16 | (1333 bp) at 25 min (dry) (916 bp) at 0 min (dry) (573 bp) at 45 min (dry) (310, 164 bp) at 25 min (wetted) (285 bp) at 45 min (wetted) | 76.2 |
9 | ISSR-16 | 438–1618 bp | 11 | 1 | 10 | (1681 bp) at 25 min (wetted) | 90.91 |
10 | ISSR-HB9 | 201–674 bp | 7 | 1 | 6 | (201 bp) at 90 min (dry) | 85.7 |
11 | ISSR-HB13 | 288–871 bp | 12 | - | 12 | (524, 418 bp) at 25 min (dry) | 100 |
12 | ISSR-HB14 | 305–1189 bp | 7 | 2 | 5 | (1189 bp) at 25 min (wetted) (1092 bp) at 0 min (wetted) | 71.4 |
Total number of bands | – | 172 | 81 | 91 | 19 | 52.91 | |
Average | – | 14.33 | 6.75 | 7.58 | 1.6 | – | |