Primer | Oligos | Tm (C) | Ta (C) | TF (no.) | PF (no.) | Fragment sizes | PIC |
---|---|---|---|---|---|---|---|
HFO-4 | GGCGGCGG | 41.9 | 41 | 11 | 10 | 155, 190, 240, 295, 360, 395, 430, 510, 620, 710, 800 | 0.8878 |
HFO-7 | GCCGCCGC | 43.5 | 50 | 8 | 7 | 190, 310, 390, 470, 530, 600, 685, 720 | 0.8364 |
HFO-8 | GCGGCGGC | 43.5 | 50 | 11 | 10 | 165, 195, 270, 340, 450, 490, 560, 600, 740, 800, 845 | 0.8844 |
HFO-13 | TCCGCCGC | 38.4 | 45 | 4 | 4 | 200, 310, 500, 565 | 0.7474 |
HFO-14 | GCGGCGGA | 38.4 | 45 | 6 | 6 | 187, 275, 410,490, 540, 645 | 0.7788 |
HFO-49 | GCGGCGGT | 39.1 | 45 | 7 | 7 | 309, 445, 725, 810, 840, 875, 900 | 0.8306 |
HFO-50 | ACCGCCGC | 39.1 | 45 | 7 | 7 | 310, 405, 530, 580, 630, 650, 740 | 0.8358 |
HFO-51 | TCGCCGCCG | 46.1 | 50 | 6 | 5 | 185, 220, 290, 350, 440, 520 | 0.7225 |
HFO-63 | GCCGGCGA | 38.4 | 45 | 5 | 5 | 435, 540, 600, 680, 800 | 0.7721 |
HFO-67 | GCCGCTGC | 36.8 | 45 | 4 | 4 | 250, 310, 480, 530 | 0.8614 |
HFO-68 | GCAGCGGC | 36.8 | 45 | 8 | 8 | 350, 420, 570, 680, 720, 800, 840, 900 | 0.8575 |
HFO-71 | CCACCGCCG | 42.4 | 45 | 8 | 8 | 420, 500, 570, 630, 680, 710, 800, 900 | 0.8403 |
HFO-72 | CGGCGGTGG | 42.4 | 45 | 11 | 11 | 180, 235, 320, 550, 640, 690, 740, 780, 800, 910, 980 | 0.8940 |
HFO-76 | GCCGGCGG | 41.9 | 45 | 7 | 7 | 200, 300, 365, 430, 560, 675, 710 | 0.8453 |
HFO-77 | CCTCCGCCG | 41.2 | 45 | 4 | 4 | 200, 235, 450, 675 | 0.7239 |
Total | – | – | – | 107 | 103 |  | 12.3182 |
Maxi. | Â | Â | Â | 11 | 11 | Â | 0.8940 |
Min. | Â | Â | Â | 4 | 4 | Â | 0.7225 |
Mean | – | – | – | 7133 | 6.866 |  | 0.8212 |