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Table 2 The fifteen HFO-TAG markers that were tested in pumpkin genotypes

From: Using high frequency oligonucleotides-targeting active gene (HFO-TAG) markers for genetic evaluation among genotypes (Cucurbita pepo L. and C. maxima L.)

PrimerOligosTm (C)Ta (C)TF (no.)PF (no.)Fragment sizesPIC
HFO-4GGCGGCGG41.9411110155, 190, 240, 295, 360, 395, 430, 510, 620, 710, 8000.8878
HFO-7GCCGCCGC43.55087190, 310, 390, 470, 530, 600, 685, 7200.8364
HFO-8GCGGCGGC43.5501110165, 195, 270, 340, 450, 490, 560, 600, 740, 800, 8450.8844
HFO-13TCCGCCGC38.44544200, 310, 500, 5650.7474
HFO-14GCGGCGGA38.44566187, 275, 410,490, 540, 6450.7788
HFO-49GCGGCGGT39.14577309, 445, 725, 810, 840, 875, 9000.8306
HFO-50ACCGCCGC39.14577310, 405, 530, 580, 630, 650, 7400.8358
HFO-51TCGCCGCCG46.15065185, 220, 290, 350, 440, 5200.7225
HFO-63GCCGGCGA38.44555435, 540, 600, 680, 8000.7721
HFO-67GCCGCTGC36.84544250, 310, 480, 5300.8614
HFO-68GCAGCGGC36.84588350, 420, 570, 680, 720, 800, 840, 9000.8575
HFO-71CCACCGCCG42.44588420, 500, 570, 630, 680, 710, 800, 9000.8403
HFO-72CGGCGGTGG42.4451111180, 235, 320, 550, 640, 690, 740, 780, 800, 910, 9800.8940
HFO-76GCCGGCGG41.94577200, 300, 365, 430, 560, 675, 7100.8453
HFO-77CCTCCGCCG41.24544200, 235, 450, 6750.7239
Total107103 12.3182
Maxi.   1111 0.8940
Min.   44 0.7225
Mean71336.866 0.8212
  1. Tm: Melting temperature, Ta: Annealing temperature, TF: The total number of alleles, PF: The number of polymorphism