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Table 2 The fifteen HFO-TAG markers that were tested in pumpkin genotypes

From: Using high frequency oligonucleotides-targeting active gene (HFO-TAG) markers for genetic evaluation among genotypes (Cucurbita pepo L. and C. maxima L.)

Primer

Oligos

Tm (C)

Ta (C)

TF (no.)

PF (no.)

Fragment sizes

PIC

HFO-4

GGCGGCGG

41.9

41

11

10

155, 190, 240, 295, 360, 395, 430, 510, 620, 710, 800

0.8878

HFO-7

GCCGCCGC

43.5

50

8

7

190, 310, 390, 470, 530, 600, 685, 720

0.8364

HFO-8

GCGGCGGC

43.5

50

11

10

165, 195, 270, 340, 450, 490, 560, 600, 740, 800, 845

0.8844

HFO-13

TCCGCCGC

38.4

45

4

4

200, 310, 500, 565

0.7474

HFO-14

GCGGCGGA

38.4

45

6

6

187, 275, 410,490, 540, 645

0.7788

HFO-49

GCGGCGGT

39.1

45

7

7

309, 445, 725, 810, 840, 875, 900

0.8306

HFO-50

ACCGCCGC

39.1

45

7

7

310, 405, 530, 580, 630, 650, 740

0.8358

HFO-51

TCGCCGCCG

46.1

50

6

5

185, 220, 290, 350, 440, 520

0.7225

HFO-63

GCCGGCGA

38.4

45

5

5

435, 540, 600, 680, 800

0.7721

HFO-67

GCCGCTGC

36.8

45

4

4

250, 310, 480, 530

0.8614

HFO-68

GCAGCGGC

36.8

45

8

8

350, 420, 570, 680, 720, 800, 840, 900

0.8575

HFO-71

CCACCGCCG

42.4

45

8

8

420, 500, 570, 630, 680, 710, 800, 900

0.8403

HFO-72

CGGCGGTGG

42.4

45

11

11

180, 235, 320, 550, 640, 690, 740, 780, 800, 910, 980

0.8940

HFO-76

GCCGGCGG

41.9

45

7

7

200, 300, 365, 430, 560, 675, 710

0.8453

HFO-77

CCTCCGCCG

41.2

45

4

4

200, 235, 450, 675

0.7239

Total

–

–

–

107

103

 

12.3182

Maxi.

   

11

11

 

0.8940

Min.

   

4

4

 

0.7225

Mean

–

–

–

7133

6.866

 

0.8212

  1. Tm: Melting temperature, Ta: Annealing temperature, TF: The total number of alleles, PF: The number of polymorphism