From: Rutin accumulation in gardenia calli cultures as a response to phenyl alanine and salicylic acid
Treatments | Total phenol (mg GAE/ g DW of callus) | ||
---|---|---|---|
 | First harvest (after 2 weeks of culturing) | Second harvest (after 4 weeks of culturing) | Third harvest (after 5 weeks of culturing) |
Control | 1.72 ± 0.11 | 6.20 ± 1.23 | 5.39 ± 1.21 |
2 mg/l phenylalanine | 4.36 ± 0.32 | 5.76 ± 0.04 | 7.32 ± 2.96 |
3 mg/l phenylalanine | 2.03 ± 0.06 | 5.98 ± 0.46 | 9.41 ± 0.36 |
4 mg/l phenylalanine | 2.53 ± 0.14 | 5.14 ± 0.23 | 9.52 ± 0.51 |
5 mg/l phenylalanine | 2.62 ± 0.04 | 5.69 ± 0 | 9.53 ± 0.55 |
0.25 mM salicylic acid (34.5 mg/l) | 2.01 ± 0.23 | 1.96 ± 0.92 | 4.19 ± 2.33 |
0.5 mM salicylic acid (69 mg/l) | 1.19 ± 0.23 | 1.56 ± 0.122 | 2.16 ± 0.93 |
0.75 mM salicylic acid (103.5 mg/l) | 2.03 ± 1.15 | 1.47 ± 0.53 | 1.52 ± 1.06 |
1 mM salicylic acid (138 mg/l) | 1.36 ± 0.05 | 1.18 ± 0.47 | 1.15 ± 0.19 |