Table 1 Identification of Fusarium taxa isolated from the selected wheat fields based on the sequencing of rDNA ITS1-5.8S-ITS4 region
Isolate | NCBI Blast identification | Accession no. | % ID | Field | Source | Primer | E.V |
|---|---|---|---|---|---|---|---|
TS-3 | F. chlamydosporum | MK271097 | 100 | N | Head | ITS1 | 0.0 |
ITS-6 | F. nygamai | MK271272 | 100 | N | Root | ITS1 | 0.0 |
ITS-7 | F. equiseti | MK271114 | 100 | N | Root | ITS1 | 0.0 |
ITS-8 | F. chlamydosporum | MK271104 | 100 | N | Root | ITS1 | 0.0 |
ITS-10 | F. equiseti | MK271115 | 100 | QM | Root | ITS1 | 0.0 |
ITS-13 | F. chlamydosporum | MK271105 | 100 | HM | Root | ITS1 | 0.0 |
ITS-14 | F. equiseti | MK271111 | 100 | H | Head | ITS1 | 0.0 |
ITS-15 | F. chlamydosporum | MK271100 | 100 | HM | Stem | ITS1 | 0.0 |
ITS-19 | F. graminearum | MK271263 | 100 | TI3 | Rhizo | ITS1 | 0.0 |
ITS-20 | F. pseudograminearum | MK271243 | 100 | MSR | Root | ITS1 | 0.0 |
ITS-21 | F. culmorum | MK281297 | 100 | D | Head | ITS1 | 0.0 |
ITS-23 | F. pseudograminearum | MK271241 | 100 | MSR | Stem | ITS1 | 0.0 |
ITS-24 | F. pseudograminearum | MK271246 | 100 | TK | Seed | ITS1 | 0.0 |
ITS-29 | F. solani | MK271231 | 100 | TI1 | Head | ITS1 | 0.0 |
ITS-30 | F. solani | MK271232 | 100 | D | Head | ITS1 | 0.0 |
ITS-33 | F. graminearum | MK271264 | 100 | N | Rhizo | ITS1 | 0.0 |
ITS-34 | F. avenaceum | MK271268 | 100 | MSP | Root | ITS1 | 0.0 |
ITS-35 | F. avenaceum | MK271267 | 100 | N | Stem | ITS1 | 0.0 |
ITS-36 | F. graminearum | MK271248 | 100 | H | Stem | ITS1 | 0.0 |
ITS-37 | F. graminearum | MK271249 | 100 | QRS | Stem | ITS1 | 0.0 |
ITS-38 | F. graminearum | MK271250 | 100 | QRS | Stem | ITS1 | 0.0 |
ITS-39 | F. graminearum | MK271259 | 100 | N | Root | ITS1 | 0.0 |
ITS-40 | F. culmorum | MK281298 | 100 | HM | Stem | ITS1 | 0.0 |
ITS-41 | F. chlamydosporum | MK271106 | 100 | HM | Root | ITS1 | 0.0 |
ITS-42 | F. chlamydosporum | MK271107 | 100 | LM1 | Root | ITS1 | 0.0 |
ITS-43 | F. chlamydosporum | MK271109 | 100 | HM | Rhizo | ITS1 | 0.0 |
ITS-52 | F. solani | MK271235 | 100 | TI1 | Stem | ITS1 | 0.0 |
ITS-54 | F graminearum | MK271260 | 100 | LM2 | Root | ITS1 | 0.0 |
ITS-56 | F. avenaceum | MK271269 | 100 | TI3 | Rhizo | ITS1 | 0.0 |
ITS-57 | F. chlamydosporum | MK271101 | 100 | MSP | Stem | ITS1 | 0.0 |
ITS-59 | F. graminearum | MK271265 | 100 | LM2 | Rhizo | ITS1 | 0.0 |
ITS-63 | F. culmorum | MK281299 | 100 | D | Head | ITS1 | 0.0 |
ITS-64 | F. graminearum | MK271261 | 100 | TI3 | Root | ITS1 | 0.0 |
ITS-67 | F. graminearum | MK271247 | 100 | QRS | Head | ITS1 | 0.0 |
ITS-68 | F. pseudograminearum | MK271239 | 100 | TI1 | Head | ITS1 | 0.0 |
ITS-69 | F. culmorum | MK281300 | 100 | TI3 | Rhizo | ITS1 | 0.0 |
ITS-71 | F. graminearum | MK271251 | 100 | TI3 | Stem | ITS1 | 0.0 |
ITS-72 | F. chlamydosporum | MK271102 | 100 | TI2 | Stem | ITS1 | 0.0 |
ITS-73 | F. solani | MK271236 | 100 | TI2 | Root | ITS1 | 0.0 |
ITS-74 | F. culmorum | MK281301 | 100 | TI2 | Root | ITS1 | 0.0 |
ITS-75 | F. culmorum | MK281302 | 100 | MSP | Stem | ITS1 | 0.0 |
ITS-77 | F. culmorum | MK281303 | 100 | MSP | Root | ITS1 | 0.0 |
ITS-79 | F. culmorum | MK281304 | 100 | TI3 | Stem | ITS1 | 0.0 |
ITS-80 | F. pseudograminearum | MK271244 | 100 | TI3 | Root | ITS1 | 0.0 |
ITS-86 | F. equiseti | MK271113 | 100 | HM | Stem | ITS1 | 0.0 |
ITS-88 | F. chlamydosporum | MK271098 | 100 | LM1 | Head | ITS1 | 0.0 |
ITS-92 | F. solani | MK271238 | 100 | QM | Rhizo | ITS1 | 0.0 |
ITS-94 | F. chlamydosporum | MK271108 | 100 | N | Root | ITS1 | 0.0 |
ITS-97 | F. chlamydosporum | MK271103 | 100 | QM | Stem | ITS1 | 0.0 |
ITS-99 | F. culmorum | MK281305 | 100 | ML2 | Root | ITS1 | 0.0 |
ITS-103 | F. graminearum | MK271252 | 100 | QRS | Stem | ITS1 | 0.0 |
ITS-105 | F. graminearum | MK271253 | 100 | TI3 | Stem | ITS1 | 0.0 |
ITS-106 | F. pseudograminearum | MK271240 | 100 | LM1 | Head | ITS1 | 0.0 |
ITS-107 | F. pseudograminearum | MK271245 | 100 | LM2 | Root | ITS1 | 0.0 |
ITS-108 | F. pseudograminearum | MK271242 | 100 | MSP | Stem | ITS1 | 0.0 |
ITS-109 | F. graminearum | MK271254 | 100 | LM2 | Stem | ITS1 | 0.0 |
ITS-110 | F. graminearum | MK271262 | 100 | N | Root | ITS1 | 0.0 |
ITS-112 | F. cerealis | MK271270 | 100 | QRS | Root | ITS1 | 0.0 |
ITS-114 | F. graminearum | MK271255 | 100 | QRS | Stem | ITS1 | 0.0 |
ITS-115 | F. cerealis | MK271271 | 100 | QRS | Root | ITS1 | 0.0 |
ITS-118 | F. culmorum | MK281306 | 100 | QRS | Root | ITS1 | 0.0 |
ITS-119 | F. equiseti | MK271112 | 100 | LM1 | Head | ITS1 | 0.0 |
ITS-122 | F. graminearum | MK271256 | 100 | TI2 | Stem | ITS1 | 0.0 |
ITS-123 | F. graminearum | MK271257 | 100 | TI2 | Stem | ITS1 | 0.0 |
ITS-125 | F. graminearum | MK271258 | 100 | H | Stem | ITS1 | 0.0 |
ITS-126 | F. avenaceum | MK271266 | 100 | D | Head | ITS1 | 0.0 |
ITS-129 | F. solani | MK271237 | 100 | TI1 | Root | ITS1 | 0.0 |
ITS-130 | F. solani | MK271233 | 100 | TI1 | Head | ITS1 | 0.0 |
ITS-131 | F. chlamydosporum | MK271110 | 100 | TK | Soil | ITS1 | 0.0 |
ITS-142 | F. cf. solani | MK271234 | 100 | TI1 | Head | ITS1 | 0.0 |
ITS-146 | F. chlamydosporum | MK271099 | 100 | TK | Head | ITS1 | 0.0 |