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Table 2 Annealing program

From: Generation of CRISPR-cas9 construct for knockout of genes encoding chromatin-associated proteins

98 °C 1 min
98–88 °C 5 s, decrease 0.1 °C/cycle × 99 cycles
88–78 °C 10 s, decrease 0.1 °C/cycle × 99 cycles+
78–68 °C 10s, decrease 0.1 °C/cycle × 99 cycles
68–58 °C 10s, decrease 0.1 °C/cycle × 99 cycles
58–48 °C 10 s, decrease 0.1 °C/cycle × 99 cycles
48–38 °C 10 s, decrease 0.1 °C/cycle × 99 cycles
38–18 °C 10 s, decrease 0.1 °C/cycle × 99 cycles
18 °C Forever