Table 2 Annealing program
From: Generation of CRISPR-cas9 construct for knockout of genes encoding chromatin-associated proteins
98 °C | 1 min |
|---|---|
98–88 °C | 5 s, decrease 0.1 °C/cycle × 99 cycles |
88–78 °C | 10 s, decrease 0.1 °C/cycle × 99 cycles+ |
78–68 °C | 10s, decrease 0.1 °C/cycle × 99 cycles |
68–58 °C | 10s, decrease 0.1 °C/cycle × 99 cycles |
58–48 °C | 10 s, decrease 0.1 °C/cycle × 99 cycles |
48–38 °C | 10 s, decrease 0.1 °C/cycle × 99 cycles |
38–18 °C | 10 s, decrease 0.1 °C/cycle × 99 cycles |
18 °C | Forever |