Table 3 Detailed information of the primers used for genotyping
Name of SSR marker | Nucleotide sequence | Chromosome NO. | Repeat motif | Anneal temp (°C) | Expected product size (bp) | |
|---|---|---|---|---|---|---|
Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) | |||||
RM107 | AGATCGAAGCATCGCGCCCGA | ACTGCGTCCTCTGGGTTCCCGG | 9 | (GA)7 | 67 | 189 |
RM242 | GGCCAACGTGTGTATGTCTC | TATATGCCAAGACGGATGGG | 9 | (CT)26 | 55 | 225 |
RM3825 | AAAGCCCCCAAAAGCGGTAC | GTGAAACTCTGGGGTGTTCG | 1 | (GA)21 | 55 | 147 |
RM271 | TCAGATCTACAATTCCATCC | TCGGTGAGACCTAGAGAGCC | 10 | (GA)15 | 55 | 101 |
RM136 | GAGAGCTCAGCTGCTGCCTCTAGC | GAGGAGCGCCACGGTGTACGC | 6 | (AGG)7 | 55 | 101 |
RM5443 | TACGGCTTACCCATAGCAGC | AAACGGAGGGAGTATTTCCC | 1 | (TC)17 | 50 | 147 |
RM321 | CCAACACTGCCACTCTGTTC | GAGGATGGACACCTTGATCG | 9 | (CAT)5 | 55 | 200 |
RM553 | AACTCCACATGATTCCACCC | GAGAAGGTGGTTGCCAGAAGC | 9 | (CT)10 | 55 | 162 |
OSR28 | AGCAGCTATAGCTTAGCTGG | ACTGCACATGAGCAGAGACA | 9 | (AGA)n | 55 | 150 |
Details of the primer pairs specifically lined to LEA genetic locus | ||||||
Marker name | Forward primer | Reverse primer | Chr. No. | Tm (°C) | Expected product size (bp) | |
LEA | 5′GCTTAGGATCAATGGCTTCCCACC3 ′ | 5′CCAAAGGGAAATCATTCACGGCGTC3 ′ | 59 | 900 | ||