Elucidation of genetic diversity among some accessions of sugar beet (Beta vulgaris L.) using inter-simple sequence repeats (ISSR) markers

Sugar beet is considered a very important strategic economic crop, as it comes at the second place for white sugar production in Egypt after sugar cane and the sugar percentage in its roots is 20%. This work was conducted in Egypt as a direct response to bridge the gap which began to expand significantly between the production and consumption of white sugar and the desire for the genetic improvement process to increase the productivity of this crop. Further, as serious attempt to understand the nature of the genetic diversity among a group of sugar beet accessions with different origins, it constitutes an important source for establishing a program of breeding and improving sugar beet crop under Egyptian conditions. The process of verifying the existence of significant genetic variation between the eight sugar beet varieties with various origins was carried out through a set of genetic parameters through two growing seasons besides, the data of ISSR markers profile. Further, all the sugar beet varieties were superior in all the studied traits during their evaluation over two agricultural years, where the two sugar beet accessions Oscar Poly and Rass Poly are superior in TSS % and sucrose % traits, Mont Bianco and Sultan in apparent purity % trait, Blino and Sultan in root yield and top yield traits and Rass Poly and Blino in sugar yield trait, respectively. In addition, the values of heritability in broad sense were high in all studied traits except the two traits; sucrose % and sugar yield where their results were appeared medium during both growing seasons. In the same regard, expected genetic advance values showed significant genetic progress in all studied traits during the two growing seasons. As well, highly genetic diversity was obtained between the eight sugar beet accessions through generated 169 amplified fragments from the twelve ISSR primers, 122 of them were polymorphic with 72.18% polymorphism. The eight sugar beet varieties achieved remarkable and distinctive results for all traits under study during two agricultural years. However, they were differentiated and various from each other. As they differed in their superiority in all estimated traits and they proved to be fertile material for studying in this investigation and very suitable for enriching the breeding and genetic improvement program to promote the sugar beet yield in order to increase the productivity of white sugar in Egypt.

addition, sugar beet accounts about 25% of the total global demand for white sugar (Draycott 2006) and at present, this percentage may range from 40 to 45%. As well, sugar beet is deeming the second important source for sugar production not only in Egypt, but internationally. Nearly from 66% of white sugar production in Egypt comes from both sugar cane and sugar beet. Therefore, the Egyptian state resorted to compensating for this lack of local production by importing from several countries and of course this constitutes a great stress on the Egyptian economy. Whatever, increasing the cultivated area besides, increasing the sugar production per unit area is an imperative and necessary to bridge the gap between the production and consumption of white sugar in Egypt.
Two distinct subgroups of sugar beet matching together with good form by using 23 SSR markers were discovered by Li et al. (2010). Knowledge of genetic diversity and the mechanisms of trait inheritance are among the most important scientific bases that can be used in the simple selection process for the purpose of genetic development and improvement in plant breeding programs (Izadi-Darbandi et al. 2013). Abbasi et al. (2014) revealed the genetic diversity in sugar beet through estimating some agro-morphological traits and SSR markers and detected that 43 and 32.6% of the total genetic variability in agromorphological attributes could recognize salt-stress and drought tolerant parents. Ganapati et al. (2015) studied the genetic variability, heritability and genetic advance in quantitative traits of some sugar beet accessions through using gamma rays and clarified the genetic diversity and genetic improvement induced through the mutations in M1 generation.
Genetic diversity and relationship in sugar beet pollinators based on SSR markers were reported by Taski-Ajdukovic et al. (2017) whom discovered 129 alleles with average of 3.2 alleles for each SSR marker responsible for genetic diversity among sugar beet genotypes. Genetic diversity between planted beets (Beta vulgaris) evaluated via population-across whole genome sequence by Galewski and Mcgrath (2020) which they confirmed that the relative contributions of specific chromosomes were succeed to genome wide compare, where each chromosome detecting a various pattern of differentiation with related to crop type. Abou-Elwafa et al. (2020) studied genetic diversity in some sugar beet accessions under various conditions and showed that the two sugar beet accessions, namely USKPS25 and USC944-6-68 exhibited highly yield, quality and tolerance under both irrigation conditions (irrigated and water stress) and heat stress and must be exploited these materials in the sugar beet breeding and improvement programs.
For the sake of genetic improvement to increase the production of white sugar in this important crop, it was necessary to import a large group of genetic materials superior in sugar production and cultivate them under Egyptian conditions to test the degree of their adaptation to the environment and study the genetic diversity of these imports. As a serious attempt to benefit from it in a program of breeding and improving the sugar beet crop and increasing its final production from the roots and then producing white sugar.

Genetic materials
The present investigation was carried out in the research station in Aga center, Dakahlia Governorate, Egypt using eight sugar beet accessions with different origin in seasons 2017/2018 and 2018/2019. This study aimed to study the genetic diversity, genetic behavior and the extent of adaptation to the Egyptian environment in 8 sugar beet genotypes (Table 1). The preceding summer crop was rice in both seasons.

Sowing
Sugar beet balls (3-5 balls/hill) were hand sown using dry planting method on one side of the ridge in hills 20 cm apart in the half of October in both growing seasons and irrigation process was done immediately after Irrigation method Sprinkler irrigation.

Studied traits
At harvest time, 15 plants were randomly chosen from the outer ridges of each replicate to determine the following traits: 1. TSS % it was calculated in juice of fresh roots by using hand refract meter. 2. Sucrose % it was determined Polari metrically on lead acetate extract of fresh maceleveld roots according to the method of Curruthers and Oldfield (1961). 3. Apparent purity % it was calculated as a ratio between sucrose % and TSS % of roots as the method outlined by Curruthers and Oldfield (1961).
Plants that produced from the two inner ridges of each replicate at harvesting time were collected and cleaned. Then, roots and tops were sepaleveld and weighted in Kg and converted to calculate: 4. Root yield (t/fed). 5. Top yield (t/fed). 6. Sugar yield (t/fed) it was measured by multiplying root yield by sucrose %.

Statistical analysis
Data obtained from all studied traits of the eight sugar beet accessions were analyzed using the SPSS ver.17, and analysis of variance was detected as recorded by Gomez and Gomez (1984).

Estimates of genetic parameters
Variance components, heritability in broad sense, genetic coefficient of variability (GCV %), phenotypic coefficient of variability (PCV %), D z or the difference between the phenotypic coefficient of variation (PCV %) and genotypic coefficient of variation (GCV %), expected genetic advance (GA) in addition, the genetic advance as percentage of mean (GAM) were the most important measurements calculated through the two seasons in this study as follows: The genetic coefficient of variability (GCV %) and phenotypic coefficient of variability (PCV %) were estimated according to the method suggested by Burton and Devane (1953). This evidence is considered extremely important because genotypic coefficient of variation largely represents the role of the additive, dominance and their various interactions besides, their direct impact on the process of inheriting important attributes, which represent the top goals of breeding for plant breeders for improving sugar beet yield. As for the phenotypic coefficient of variance, it represents the sum of the environmental and genetic variance, specifically the limit of the environmental impact or the environmental error, which in most cases must be very small to a negligible degree that does not affect the inheritance of important quantitative traits.
Estimation of heritability in broad sense Broad sense heritability (h 2 ) expressed as the percentage of the ratio of the genotypic variance (g v) to the phenotypic variance (ph v) and was estimated on genotype mean basis as described by Burton andde Vane (1953) andJonhson et al. (1955). D z The difference between the phenotypic coefficient of variation (PCV %) and genotypic coefficient of variation (GCV %).
Estimation of genetic advance The expected genetic advance (GA) and percentage of the mean (GAM) assuming selection of superior 5% of the genotypes was estimated in accordance with the methods illustrated by Johnson et al. (1955).

Molecular characterization
Molecular genetics this important branch of genetics is a very important and essential step to explore and clarify the genetic differences between the eight genotypes of sugar beet, but on the molecular level. This work comes in the second place in the midst of the objectives of this investigation for determining degrees of genetic diversity among 8 sugar beet accessions. Besides, indicating the extent of compatibility and differences between these varieties with different origin. On this basis, the use of molecular genetic markers was not only evidence for the genetic differences between these sugar beet genotypes, but served as the taxonomic basis for these genetic materials. In addition, it greatly emphasized their great efficiency in terms of environmental adaptation. From this point of view, 12 ISSR primers, which had the greatest merit in clarifying and discovering the most important molecular genetic differences between the eight sugar beet varieties, were used in this context in Table 2. In addition, drawing the phylogenetic tree or cluster analysis for the genetic similarity among them and the following is a detailed explanation in this regard.
Note that, from the scientific and practical secretariat, it can be said that a large number of ISSR primers were Page 4 of 17 El-Mouhamady et al. Bull Natl Res Cent (2021) 45:166 used, more than 12 primers. But, the 12 ISSR primers only used in this investigation were indeed the ones that succeeded in finding genetic differences and achieved the successful comparison between the eight sugar beet cultivars, which showed great benefit in this regard.

DNA extraction and PCR
The fresh leaves of the eight sugar beet plants was frozen in liquid nitrogen and ground to a fine powder using prechilled mortar and pestle. The powdered material was used for genomic DNA extraction by DNeasy kit following the manufacturer's instructions. The obtained DNA purity and concentration were determined by running on 1% agarose gel and using Nanophotometer.

Detection of the PCR products
The amplification products were resolved by electrophoresis in a 1.5% agarose gel containing ethidium bromide (0.5ug/ml) in 1X TBE buffer at 95 V.

Data handling and cluster analysis (Phylogenetic tree)
Data were scored for computer analysis on the basis of the presence or absence of the amplified products for each primer. Pairwise components of the eight sugar beet accessions based on the presence or absence of unique and shared polymorphic products, were used to determine similarity coefficients according to Jaccard (1908). The similarity coefficients were then used to construct dendrograms, using the unweighted pair group method with arithmetic averages (UPGMA) employing the SAHN (Sequential, Agglomerative, Hierarchical and Nested clustering) from the NTSYS-PC (Numerical Taxonomy and Multivariate Analysis System), version 1.80 (Applied Biostatistics Program).

Analysis of variance (ANOVA Test)
Results viewed in Table 3 and associated with analysis of variance test showed highly significant differences between the eight sugar beet genotypes for the

Mean performance
The mean values of all studied attributes for both growing seasons (2017/2018 and 2018/2019) were presented in Table 4  .72 ton/fed), followed by Pita poly terry (13.84 and 14.07 ton/fed), followed by Oscar Boly (11.82 and 11.82 ton/fed), followed by Baraca (12.09 and 12.24 ton/fed), followed by Mont Bianco (10.03 and 10.03 ton/fed) and then followed by Rass poly (9.62 and 10.02 ton/fed) for the two growing seasons, respectively. Concerning sugar yield trait, the sugar beet cultivar Rass poly was exhibited the highest mean values of this trait for both years (5.69 and 5.51 ton/fed), followed by Blino (5.34 and 5.32 ton/fed), followed by Oscar Boly (5.24 and 5.28 ton/fed), followed by Sultan (5.05 and 4.92 ton/fed), followed by B (4.83 and 4.77 ton/fed), followed by Pita poly terry (4.54 and 4.53 ton/fed), followed by B2001 (4.29 and 4.48 ton/fed) and then followed by Mont Bianco (3.85 and 3.93 ton/fed) for the two growing seasons, respectively, in Table 4.

Genetic components
Data presented in Table 5 showed that the values of heritability in broad sense were appeared high in the studied attributes; TSS % (82.35% and 82.10%), apparent purity % (82.47% and 75.65%), root yield (84.87% and 86.76%) and top yield (85.30% and 80.52%) in both growing seasons, respectively, while they were appeared medium in the traits; sucrose % (51.42% and 61.97%) and sugar yield (51.41% and 66.83%) in both years, respectively. In generally, it is natural that there are differences between PCV and GCV values for all the studied traits during the two years of the study because these differences are very importance and confirm beyond any doubt that the genetic variance occupied the greatest part of the total level of phenotypic variance when these differences being little and this is strongly reflected in the weakness of the environmental variance. In the same context, it is attributed to the growing values of the degree of heritability in the broad sense especially for yield and its components of sugar beet. On the other hand, vice versa if the differences between them are great. In this investigation, the values of genotypic variance were higher than the values of environmental variance in all studied traits for the two growing seasons. Therefore, the major part of the phenotypic variance was the share of the genetic variance. In the same regard, the estimated values of PCV % were bigger than its peers of GCV % in all studied traits for the two seasons, where the values of PCV % were high in the traits; sugar yield (36.82% and 41.21%), followed by top yield (26.39% and 21.15%), followed by TSS % (13.40% and 12.56%), followed by root yield (11.21% and 11.64%), followed by sucrose % (10.64% and 12.43%) and then followed by apparent purity % (2.43% and 2.15%) in both growing seasons, respectively. In the same context, the differences between phenotypic and genotypic coefficient of variation (D Z ) were low in all studied traits in both years except sugar yield trait where it recorded (10.42 and 7.52) in the two growing seasons, respectively. As was briefly explained earlier, the values of D z represent the final criterion for the differences between PCV and GCV %. If these differences are large, this will reduce the values of the degree of heritability due to the presence of a large role for environmental variance. But, if it is small, which is the desired case, this confirms the large degrees of heritability in broad sense for all quantitative traits. In addition, the absence of the role of the environment in changing the nature and shape of all attributes under study. Data assessment of expected genetic advance (GA) based on 5% selection revealed various results for all studied traits in both growing seasons, where it was medium in the traits; TSS % (4.60% and 10.81%), root yield (4.96% and 5.28%) and top yield (6.05% and 12.13%) and appeared low in the rest traits, namely sucrose % (2.18% and 3.05%), apparent purity % (3.97% and 3.21%) and sugar yield (1.89% and 2.75%), respectively. It is worth mentioning that genetic advance is one of the most important genetic measurements that are used to know the extent and size of change in the performance of important quantitative traits such as resistance to biotic and abiotic stresses. As well as, strategic yield traits, especially sugar attributes in this regard and their reach to a positive direction, which in turn is reflected in the final yield for plant breeders, as well as for both the farmer and the consumer. Accordingly, the aim of using it in this study is to find out to what extent these imported sugar beet genotypes have reached the genetic stability in yield and its components and to ensure its high performance under the Egyptian environment conditions. This is what makes it more important in this context. With respect to GAM %, the first rank of this genetic parameter was observed in top yield trait (46.53% and 95.06%), followed by sugar yield trait (38.96% and 56.81%), followed by TSS % trait (22.80% and 53.83%), followed by root yield trait (19.65% and 20.86%), followed by sucrose % trait (11.29% and 15.91%) and then followed  by apparent purity % trait (4.15% and 3.36%) for the two growing seasons, respectively.

Profile of ISSR analysis
The twelve ISSR primers used for comparing among the eight sugar beet accessions, namely ISSR-1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 and 13 exhibited a total of 169 fragments, 47 of them were monomorphic, while that, 122 bands were showed polymorphic with 72.18% (polymorphism) included 15 unique bands as presented in Table 6; Fig. 3. The average numbers of polymorphic ISSR markers were 10.16 fragments for each primer. Polymorphic bands number ranged from 6 to 17 and molecular size ranging from 140 to 1450 bp, respectively. The highest number of total bands was observed by primer ISSR-1 (22), followed by ISSR-2 primer (20), followed by the primers; ISSR-3 and 6 (17) fragment for both of them, followed by ISSR-4 primer (15), followed by ISSR-7 (14), followed by ISSR-10 primer (12), followed by the primers; ISSR-8 and 9 (11) bands for both of them and then followed by the primers; ISSR-5, 11 and 13 (10) fragments for them all, respectively. Further, the highest number of polymorphic bands were showed in primer ISSR-1 (17), followed by the primers ISSR-2, 3 and 6 (13) for them all, followed by ISSR-7 primer (11), followed by ISSR-11 (9), followed by the primers; ISSR 8 and 9 where they recorded 8 bands for both of them and then followed by the primers; ISSR-5 and 10 (7) fragments for both of them, respectively, while ISSR-13 primer was exhibited the lowest number of polymorphic bands (6). In the same context, the highest polymorphism % were observed in primers; ISSR-11 (90.0%), followed by ISSR-7 (78.57%), followed by ISSR-1 (77.27%), followed by ISSR-3 and 6 (76.47%) for both of them, followed by ISSR-8 and 9 (72.72%) for together, followed by ISSR-5 (70.0%), followed by ISSR-4 (66.66%), followed by ISSR-2 (65.0%), followed by ISSR-13 (60.0%) and then followed by the primer ISSR-10 (58.33%) where it recorded the lowest polymorphism % in this regard, respectively. Further, primers; ISSR-6, 3 and 5 recorded the highest number of unique bands where they exhibited (4, 3 and 2) bands for them of all, respectively. In addition, the rest primers generated only unique band except the three primers; ISSR-4, 10 and 13 where they exhibited no unique band in this investigation. Data viewed in Table 7 detected that the sugar beet cultivars B2001, Mont Bianco, Oscar Boly and Pita poly terry recorded the highest number of amplified fragments and were coming in the first rank in this regard and their values were (102, 103, 109 and 96), respectively. The other sugar beet genotypes coming in the second rank showed different number of bands. Further, primers ISSR-1, 2, 4 and 6 exhibited the highest number of bands (94, 102, 73 and 72) for each one of them in all sugar beet genotypes, while ISSR-5, 8 and 11 primers generated the lowest number of amplicons (45, 46 and 41) for all of them, respectively. In addition, the rest ISSR primers were exhibited various number of amplified fragments.
Data viewed in Table 8 showed 15 positive specific markers generated by twelve ISSR primers. These primers, namely ISSR-1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 and 13, used in the current study succeeded in discovering and comparing the molecular genetic differences among the different sugar beet genotypes Table 8. In addition, these molecular genetic differences were very important in this regard, especially since the eight sugar beet accessions came from different environmental origins and regions. As well, these molecular genetic differences are considered basic taxonomic differences among the eight sugar beet accessions. The following is a detailed explanation  -Mouhamady et al. Bull Natl Res Cent (2021) 45:166 of ISSR primers that gave positive markers in this track. ISSR-1 and 2 primers exhibited 2 positive specific marker in sugar beet genotypes; 3 and 7 with sizes of 150 and 1100 bp, besides, three positive markers with sizes of 690, 810 and 1250 bp were obtained in genotypes; 1, 2 and 6 by ISSR-3 primer, respectively. For ISSR-5 primer, two positive markers were observed in genotypes; 5 and 7 with sizes of 190 and 1000 bp. With respect to ISSR-6 primer, four positive specific markers were observed in this regard as follows; two markers with sizes of 1200 and 170 bp for genotypes; 5 and 6 besides, two markers with sizes of 330 and 1300 bp for genotype 7 only, respectively. In the same context, ISSR-7 and 8 primers produced two positive markers with sizes of 160 and 530 bp for genotype 3 only. Finally, ISSR-9 and 11 primers exhibited two positive specific markers with sizes of 620 and 209 bp for sugar beet genotypes; 6 and 8, respectively.

Proximity matrix analysis (Genetic Similarity)
Data presented in Table 9 exhibited (28) pairwise comparisons to debate the genetic relationships between the eight sugar beet genotypes revealed in terms of genetic   Table 8 Mapping of positive specific markers for the eight sugar beet genotypes using 12 ISSR primers   -Mouhamady et al. Bull Natl Res Cent (2021) 45:166 similarity. The genetic similarity values ranged from (0.876 to 0.400) with an average of (0.638), where the highest level of genetic similarity was (0.876) among (Genotype 4 and Genotype 5). While that, the lowest rank of similarity was (0.400) between (Genotype 1 and Genotype 4), respectively. Further, some genetic similarity values were showed a little above average such as the genetic relationships among (Genotype 1 and Genotype 8) (0.625), (Genotype 3 and Genotype 8) (0.651) and (Genotype 7 and Genotype 8) (0.681), respectively. The rest genetic similarity values were appeared low to medium in this regard.

Cluster analysis (Phylogenetic tree)
Results of cluster analysis or phylogenetic tree which presented in Fig. 4 divided all sugar beet genotypes into two main clusters, where the cluster I included genotypes; 2, 4 and 5, while cluster II contained genotype 6 and two sub-cluster. The sub-cluster one included one group (genotype 1 and genotype 3). Whatever, the sub-cluster number two included one group (genotype 7 and genotype 8), respectively.

Discussion
The scientific facts shown in Table 3 confirmed that the environmental variation had a very weak effect in inheriting the recent traits and that genetics played a pivotal and essential role for manufacturing highly genetic stability in these genetic materials of sugar beet. Further, this results were verified through evaluating all studied traits during two growing seasons. In addition, the great genetic diversity shown by these sugar beet accessions confirms that its use in breeding and improvement programs of sugar beet under Egyptian conditions will be a very important step in this context. These results were in agreement with those reported by (2020) and Bassiony et al. (2020). Results obtained in Table 4 and Fig. 2 confirm the existence of a large genetic diversity between the eight sugar beet accessions under evaluating. This fact was confirmed by observing all values and data for all studied characteristics of these genetic materials during the two growing seasons, where some varieties surpassed in certain traits, while the rest of sugar beet cultivars were excelled in the rest of the other attributes. In the context, the sugar beet cultivar Oscar Boly was recorded the highest rank in the traits; TSS % and sucrose % during both growing seasons, while the cultivar Mont Bianco exhibited the first rank in apparent purity % trait and the genotype Blino was shown highly data in root yield trait. Furthermore, the accessions, Sultan and Rass Boly, were recorded the highest rank for the traits; Top yield and sugar yield for the two growing seasons, respectively. For the rest of sugar beet varieties, it came in second place in terms of excellence of all studied traits. In addition, these varieties have already succeeded in environmental adaptation and genetic stability under Egyptian conditions. Accordingly, the use of sugar beet accessions that are genetically diverse and various origin had the greatest credit for the genetic improvement in all attributes under investigating, especially for each of sugar yield and root yield. Therefore, its inclusion in programs to improve and develop the characteristics of the sugar beet crop in Egypt will be a very important decision and a serious scientific step that has an economic impact in the long run. This will definitely reduce the big gap between white sugar production and consumption in Egypt. These results were in agreement with those obtained by Izadi-Darbandi et al. Results viewed in Table 5 revealed that in the case of heritability in broad sense was high in the traits; TSS %, apparent purity %, root yield and top yield for both growing seasons indicates that the genetic variation was a great influence for controlling in the inheriting of these previous traits, while the role and influence of environmental variation diminished. Whatever, in the case of heritability was medium in the studied traits; sucrose % and sugar yield in both years confirms that confirms that the genetic and environmental variation were equal in terms of influence and control in the inheritance of these two traits (El-Mouhamady 2009;El-Demardash et al. 2017;Singh et al. 2018;El-Mouhamady and El-Metwally 2020). Moreover, it was observed from the context of these results that the largest part of the total variance was from the share of the genetic variation in all the studied traits during both growing seasons. This indicates the basic, pivotal and important role of genetic variation in controlling and inheriting of all traits under studying. Besides, it confirms that the role of environmental variation has diminished in most studied traits. Therefore, breeding these traits using these different sugar beet genotypes with various origin in order to raise sugar and root yield in Egypt will be an inevitable and necessary matter at this time (Izadi-Darbandi et al. 2013;El-Mouhamady et al. 2014a, 2014bAbbasi et al. 2014;Ganapati et al. 2015;Taski-Ajdukovic et al. 2017;El-Mouhamady and Habouh 2019;Galewski and Mcgrath 2020;El-Mouhamady and El-Metwally 2020). Through results obtained in Table 5, it was noted that the PCV % values of all tested traits were greater than their counterparts in GCV % during both growing seasons. This explains that the genetic improvement of these traits was not dependent on the genotype only, but on the environment and the interaction between environmental X genotype. Thus, selection processes for development of all studied traits mentioned above through phenotype could be the fruitful goal in this direction. This greatly demonstrated the extent of the genetic stability that these genotypes enjoy. These results were in agreement with those reported by Eldessouky et al. In the same context, Singh et al. (2018) confirmed that studied traits with higher values of GCV, PCV and heritability in sugar beet related with better genetic advance and highly significant positively a complication with root yield and heaving high direct and indirect impacts are considering as selection criteria while selection to be made for higher sugar and sugar yield evaluated of genetic variability.
Values of expected genetic advance (GA) based on 5% selection for all studied traits in both years appeared low to medium confirmed that additive and non-additive types of gene action were played an importance and fruitful role for controlling and inheriting the previous traits. Thus, the simple selection process for these traits would be effective through individual plants, while calculated data of GAM % (Genetic advance as percent- One of the most positive fruits of this study was the use of different genotypes of sugar beet origin. This means that the plant breeder has already seized an opportunity to select and choice the best genes that express useful quantitative traits that can be used to establish a successful breeding and improvement program for sugar beet. Because of, the eight sugar beet varieties had already shown a large trend of genetic variation and were various from each other, although all of them were superior in all of the estimated traits. This indicates that its genetic background is very large. Therefore, it can cover all the requirements of the process of genetic improvement and promotion of the sugar beet crop under Egyptian conditions. Moreover, the genetic parameters used in this context indicated impressive results for the degree of heritability in broad sense, as it was high in most calculated traits. This confirms that additive type of gene action act was very important for inheriting and controlling the previous traits and that using them in traditional breeding programs by hybridizing them among local sugar beet varieties will be a fruitful step in improving yield and roots attributes, and then the productivity and quality of white sugar. The twelve ISSR primers used in this study were unrivaled success in finding the molecular genetic differences and determining the extent of genetic diversity between the eight sugar beet cultivars (Table 6; Fig. 3). Because of these genetic differences were considered as dividing taxonomic differences between the aforementioned sugar beet genotypes and identified 15 unique or positive specific markers generated from primers; 2,3,5,6,7,8,9 and 11 in Table 8. Therefore, these unique markers manufactured the genetic identification at the molecular level among these sugar beet accessions in this regard, (El-Mouhamady et al. 2010, 2012aAbdel Sattar and El-Mouhamady 2012;El-Seidy et al. 2013;Dohm et al. 2014;Esmail et al. 2016;Capistrano-Gossmann et al. 2017;Khatab et al. 2017;Khatab et al. 2019).
In this respect, ISSR markers had played an very importance and fruitful role to show the variability and genetic diversity which ranged from 58.33% by ISSR-10 primer to 90.0% using ISSR-11 primer and this technique was used in many studies such as in (Phaseolus vulgaris L.) by Galvan et al. (2003), in Artemisia capillaries by Shafie et al. (2009), in Lippia alba Mill by Manica-Cattani et al. (2009), in Achillea millefolium by Farajpour et al. (2012), in barley by El-Mouhamady et al. (2012b), in Lupine by Zian et al. (2013), in wheat by El-Mouhamady et al. (2014a, 2014b and Heiba et al. (2016).
As well, this study was able to determine the total number of fragments resulting from each primer for each genotype besides, referred to the detailed molecular genetic comparison among these primers. For example, ISSR-1 and 2 primers were exhibited the highest number of bands (94 and 102) for each of them in Table 7, respectively. In addition, screening the eight sugar beet varieties and knowing which is the largest in producing of amplicons like B2001 (102), Mont Bianco (103) and Oscar Boly (109). This has already participated in knowing the degrees of genetic diversity for these genotypes, especially as they are of different origin. These results were in agreement with those reported by Ganapati et al. (2015), Ramadan et al. (2016), Taski Data viewed in Table 9 explained the genetic diversity with its two types, whether it was genetic affinity or divergence. This genetic compatibility or divergence helps the plant breeder to choose genetically compatible genotypes to a large extent and this reflects the environmental compatibility in establishing a successful breeding program in which genetic improvement is among its priorities by making use of the excellent genes present in the imported varieties and responsible for tolerating to high salinity, water stress and resistance diseases through hybridization and transfer these important genes by modern biotechnological methods to local genotypes. In the same context, the genetic similarity among the eight sugar beet accessions were observed between Mont Bianco and Oscar Poly (0.876), followed by B2001 and Mont Bianco (0.863) and then followed by B2001 and Oscar Poly (0.803). While that, the lowest genetic similarity was obtained among Baraca and Mont Bianco (0.400), respectively. These results, in short, explained the importance of genetic diversity and its ratio related to the extent of genetic divergence or convergence between the previously mentioned sugar beet varieties. This will facilitate its optimal use in the sugar beet breeding and improvement program under Egyptian conditions, especially as it is superior in all the traits under studying (El-Mouhamady et al. 2012cGalewski and Mcgrath 2020).
The phylogenetic tree or cluster analysis revealed in Fig. 4 divided all sugar beet genotypes into two main cluster, where cluster one included B2001, Mont Bianco and Oscar Poly, while cluster two contained the rest of sugar beet varieties. These results confirmed that the genotypes obtained in the first cluster were genetically different and divergent from those in the second cluster. This indicates that they are actually descended from genetic origins and a different environment, which maximizes the opportunity to benefit from those foreign genes in the genetic improvement of all studied traits and the improvement of sugar beet yield under conditions Egyptian (El-Mouhamady et al. 2015;Funk et al. 2018;Abou-Elwafa et al. 2020;Mehareb et al. 2021). It is worth noting that the three aforementioned local varieties; (Baraca, B2001 and Sultan) and which fall into the two major cluster are originally imported and cultivated under Egyptian environment before the other imported lines and this is gave them the local character. Accordingly, it was necessary to test its environmental adaptation with other imported varieties and to know the degree of its genetic diversity in the presence of the other five imported cultivars. In addition, the process of producing sugar beet is difficult in Egypt, as the Egyptian environmental conditions do not suitable for it. Therefore, the use of the eight genetic materials will be a great scientific leap in breeding and improvement Egyptian sugar beet program in this regard.

Conclusion
This study attempted with a bit of verbosity to shed light on the importance of sugar beet crop and to determine the degree of genetic variation for a number of genotypes coming from different origins through two growing seasons. Likewise, this study came as a natural response to the genetic improvement in sugar beet to increase white sugar productivity in Egypt and reduce the large gap between production and consumption. Further, the strategy of this work was evaluating a set of important yield attributes of sugar beet using 8 different genotypes with various origins and knowing the degree of their environmental adaptation. Besides, determining which of them is best for enriching the breeding and genetic improvement program in this important crop and that's after determining relationships of convergence and genetic divergence among them. Heritability in broad sense, PCV %, GCV %, D z , GA (Expected genetic advance) and GAM (Genetic advance as percentage of mean %) were the most important genetic parameters calculated for all studied attributes in both growing seasons. Twelve ISSR primers were used to compare among the eight sugar beet accessions and determine the genetic diversity among them at the molecular level. The final results indicated and confirmed that the eight sugar beet varieties were significantly different from each other. This indicates that the genetic diversity among them was remarkable and they were different in their genetic behavior. In the same regard, the sugar beet accessions; Oscar poly, Mont Bianco, Blino, Sultan and Rass Boly were recorded the highest mean values for all attributes under studying in both growing seasons. Further, this distinction qualifies them to be an important resource in the breeding and genetic improvement program for sugar beet in Egypt.