Efficacy of cinnamon oil and its active ingredient (cinnamaldehyde) on the cotton mealy bug Phenacoccus solenopsis Tinsley and the predator Chrysoperla carnea

The importance of Chrysoperla carnea (Steph.) (Neuroptera: Chrysopidae) as a predator is due to nourishing this predator on some dangerous pests as the cotton mealybug, Phenacoccus solenopsis Tinsley, belongs to Hemiptera: Pseudococcidae. So the predator C. carnea was used in the management of various pests. The basic aim of this study was the indirect effect of some natural materials against some stages of C. carnea through feeding of the predator on the treated prey, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae). The results showed the comparison between the effectiveness of cinnamaldehyde and cinnamic oil in controlling the pest as soon as ineffectiveness on the predator. Cinnamaldehyde was very save against C. carnea which did not effect on the biology of it and the predator completed its life cycle as control (water + tween 80). Thus, the results suggested that cinnamaldehyde could be included in the Integrated Pest Management (IPM) Program without any adverse effect on bio-control agents used in an IPM. This study may be a great alternative to chemical pesticides in controlling cotton mealybug, P. solenopsis, and at the same time, this material is saving to the predator C. carnea. This alternative is cinnamaldehyde (the active ingredient of cinnamon oil).


Background
The cotton mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae), is considered one of the most recent invasive sap-sucking insects in Egypt that is attacking cotton (El-Zahi et al. 2016;Mostafa et al. 2018), vegetables (Ibrahim et al. 2015), and many other field crops of economic importance. This pest feeds on all the green parts of the infested plants which become stunted, weak with distorted and yellow leaves, and die in severe infestations (Culik and Gullan 2005).
Natural products have been recently attracting the attention of some scientists to avoid the problems caused by synthetic compounds (Abou-Yousef et al. 2010;Mostafa et al. 2018).
The application of predators for a successful biological control program could be controversial, due to their potential to prey on other biological control agents and non-target species (Symondson et al. 2002).
Green lacewing, Chrysoperla carnea (Stephens), is a polyphagous predator that is released for pest control in greenhouses and is also very common in many agricultural systems. The previous studies indicate that five larvae C. carnea per 100 nymphs of the pest can be used as a biological control against P. solenopsis during the management program (Ibrahim 2018). This species is a powerful agent in biological control programs because of an expanded geographical distribution, high compatibility to different systems, high searching ability, and an easy way to rear (Golmohammadi et al. 2009). C. carnea larvae are extremely effective predators in the protected or enclosed areas such as greenhouses (Nayar et al. 1976).
The aim of this study was to determine the effect of cinnamon oil and its active ingredient, Cinnamaldehyde, on the cotton mealybug, Phenacoccus solenopsis, and apply LC 50 on P. solenopsis individuals to feed the prey C. carnea and show the indirect effect of these materials on the control agent.

Insect rearing
The cotton mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae), was collected from infested cotton plants (Gossypium barbadense var. Giza 86) at the field of Aga district, Dakahalia governorate, Egypt. The mealybug was transferred to the laboratory, and sprouting potato tubers were used as a host plant for its rearing. Gravid females of P. solenopsis were inserted in sprouting potatoes. Each sprouted potato was infested with an adult female and observed daily (Attia and Ebrahim 2015). From the reared culture, newly hatched crawlers of P. solenopsis were placed on each sprouted potato before being confined in a carton cylindrical box of 8-cm long and 12-cm diameter. The carton boxes were kept at 30°C and 60 ± 5% R.H. Daily examination for the morphological changes were recorded and monitored until adult emergence (Attia and Ebrahim 2015).

Natural enemy culture maintenance
Larvae of C. carnea were obtained from the Bio-Control Laboratory of Plant Protection Research Institute, Dokki, Egypt. They were maintained on P. solenopsis at 27 ± 2°C , 65 ± 5% RH, and 16:8 L:D.

Natural products
Cinnamon oil and its active ingredient, cinnamaldehyde, were used in this study and were bought from Essential oil Extracts Center, National Research Center. Jun-Ran et al. (2015) proved that cinnamaldehyde is the active ingredient of cinnamon oil.
Cinnamaldehyde formula (Vogt 2010) Preparing the stock solution of the tested materials The stock concentrations of each tested material (cinnamon oil or cinnamaldehyde powder) were prepared on basis of weight and the volume of the distilled water (w/v) in the presence of tween 80 (0.1%) as emulsifier. Four diluted concentrations for each material were used to draw the LC-P lines. Three replicates were used for each concentration.

Toxicity test Direct experiment
Toxicity of cinnamon oil and cinnamaldehyde powder was evaluated against adult of P. solenopsis. Thirty newly emerged adults, 10 individuals in each replicate, were placed on okra leaves in each Petri dish. Each material had four concentrations, 500, 1000, 5000, and 10,000 ppm, which were sprayed on the individuals. Mortality was recorded for 7 days after treatment. The mortality percentage was estimated and corrected according to Abbott (1925). LC 50 values were determined using probit analysis statistical method of Finney (1971

Indirect experiment
After calculating LC 50 for each material, each LC 50 was sprayed on 30 adults of P. solenopsis, 10 individuals for each replicate; these individuals were introduced to one larva of the predator, C. carnea, after 24 h of direct spraying. Four replicates were used, one prey in each. The results were corrected by control, and the mortality percentage was estimated and corrected according to the analysis of variance (ANOVA) (Analytical software 2005).

Direct experiment
Toxicity studies: Effect and toxicity index of cinnamon oil and cinnamaldehyde on mortality rate of cotton mealybug,

Phenacoccus solenopsis
Ghada and Naglaa Bulletin of the National Research Centre (2020) 44:154 Results obtained in Table 1 showed that mortality rate of the active ingredient, cinnamaldehyde, was high against P. solenopsis and more effective than cinnamon oil for all concentrations used. The total mortality was 43.33, 56.67, 60, and 66.67% for 500, 1000, 5000, and 10, 000 ppm, respectively for cinnamon oil, while the total mortality for cinnamaldehyde was 50, 60, 73.33, and 76.67% for 500, 1000, 5000, and 10,000 ppm, respectively.
In addition, LC 50 of the active ingredient, cinnamaldehyde, was 423.33 ppm and LC 90 86181.21 ppm, while cinnamon oil had LC 50 802.14 ppm and LC 90 1,737, 321.67 ppm. The toxicity index was 100% for cinnamaldehyde, but it was 52.78% for cinnamon oil.
The slope values indicated that cinnamaldehyde had a higher value which was 0.555 while cinnamon oil slope value was 0.384. LC 90 /LC 50 value confirmed that cinnamaldehyde had a lower value, 203.58, than cinnamon oil, 2165.86. Thus, the highest slope value or the lowest ratio LC 90 /LC 50 means the steepest toxicity line.

Indirect experiment
In Table 2, the active ingredient, cinnamaldehyde, proved that it was a safety material against the predator C. carnea which completed its larval and pupal stages until adult emergence (C. carnea feed on P. solenopsis individuals sprayed only with water and tween 80), while using cinnamon oil completed the larval stage only of C. carnea. Also, the predation efficiency of the predator was higher by using active ingredient cinnamaldehyde than using cinnamon oil. Table 2 also demonstrated the efficiency of C. carnea female in laying eggs by percentage 100% in control as soon as in cinnamaldehyde.

Discussion
The active ingredient, cinnamaldehyde, proved its toxicity effect against P. solenopsis than cinnamon oil. Similar results were observed by Jun-Ran et al. (2015) who proved effectiveness of cinnamaldehyde and illustrated that cinnamaldehyde was a more active ingredient than other compounds of cinnamic acid which were used in controlling P. solenopsis.
Also, cinnamaldehyde is very a useful material for controlling the cotton mealy bug pest, P. solenopsis; as soon as it did not cause any harm for the predator, C. carnea. Hafiz et al. (2012) proved the effectiveness of C. carnea against P. solenopsis. Sana et al. (2015) showed that emamectin benzoate had no residual effect on the predator C. carnea than neem oil. Pilar et al. (2005) demonstrated that the natural plant extracts, Trichilia havanensis (Meliaceae) extracts and Teucrium viscidum (Lamiaceae), are nearly innocuous for both natural insects at the conditions tested. El-Wakeil et al. (2006) proved that the chemical products were harmless to adults of C. carnea. Kim et al. (2013) and Isman (2000Isman ( , 2006 illustrated that plant essential oils are potential products for the control of M. pruinosa because some of them are selective, biodegrade into nontoxic products, and have less harmful effects on non-target organisms.

Conclusion
The cotton mealy bug, Phenacoccus solenopsis, is a destructive pest for cotton and some host plants, and one of ways of controlling this pest is biological control. Chrysoperla carnea was used for predation of P. solenopsis but it dies from using the chemical pesticides, so this study is concerned mainly with maintenance of the predator, C. carnea, and at the same time controlling the pest, P. solenopsis.