Biochemical and electrophoretic study for diagnosis of the disease stage in patients with asthmatic bronchitis

Asthmatic bronchitis is a chronic allergic airway disease diagnosed after development of chronic obstructive pulmonary disease symptoms. This study was designed to reveal relation of the different electrophoretic patterns to impairment of the lung functions in patients with asthmatic bronchitis. Patients were categorized into patients at mild stage (n = 10) and those at severe stage (n = 12) in comparison to healthy group (n = 21). The major blood components, arterial blood gases and electrolytes were immediately assayed in addition to assessment of liver enzymes and kidney functions. The pulmonary indices including forced expiratory volume in one second (FEV1), forced vital capacity (FVC), ratio of FEV1 to FVC and maximal expiratory pressure (MEP) were assayed. Furthermore, protein, lipid and calcium moieties of native protein patterns in addition to isoenzemes were electrophoretically detected. It was found that liver and kidney functions were significantly (P < 0.05) elevated in asthmatic bronchitis patients. Ca2+ is the only electrolyte that decreased significantly (P < 0.05) at the severe stage. Electrophoretic patterns showed that asthmatic bronchitis disease at mild and severe stages were physiologically similar to control by 54.5 and 75.6% (protein), 73.1 and 41.6% (lipid moiety), 66.4 and 30.6% (calcium moiety), respectively. As regards to electrophoretic isoenzymes, the disease at mild and severe stages were similar to control by 95.2 and 35.4% (catalase (CAT)), 40.1 and 50.5% (peroxidase (POX)), 67.9 and 69.6% (α-esterase (α-EST)), 83.1 and 73.1% (β-esterase (β-EST)), respectively. The native electrophoretic patterns used as diagnostic tool for determining the stage at which the patients with asthmatic bronchitis exist.


Introduction
Asthmatic bronchitis is a chronic allergic airway disease involved by multiple cells (Nahori et al. 2001;Han et al. 2016). It may occur in a large number of patients who smoke cigarettes heavily. It is well known that the chronic mucous hypersecretion and airway hyperreactivity are considered as the most common features in the patients who suffer from this disease (Griffith and Garcia 1988). In addition, it is usually associated with major chronic-specific symptoms like cough and dyspnea (Molyneux et al. 2006;Yildiz and Igde 2018). It has become the most common chronic respiratory disease that may lead to mortality (Yadav and Mittal 2014).
The treatment with glucocorticoid inhalation is the most known and effective method that can suppress the rapid development of the disease and prevent asthma exacerbation but it is expressed as limited method due to the restricted knowledge of etiology about asthma whether the patients influenced by environment, exercise, mental state and other factors (Molyneux et al., 2006;Blasi et al., 2013;Yadav and Mittal, 2014;Yildiz and Igde, 2018).
Asthma is associated with defined triggers of attacks such as viral infections, dusts, fumes and allergens in addition to a positive family history (Eden et al. 2006). Various factors involved in causation of the bronchial asthma and the clinical pathogenesis of this disease is not completely known. It might be related to immune, heredity, environment and other factors (Sherrill et al., 1999;Nahori et al., 2001). Therefore, a precision approach is necessary to integrate multiple dimensions (clinical, physiological, imaging and endotyping) to move the field forward in treatment of this disease (Segal and Martinez, 2018).
Asthmatic bronchitis is diagnosed after development of chronic obstructive pulmonary disease (COPD) symptoms. It occurs in 50% of patients with α-antitrypsin deficiency (Eden, 2010). Also, it may occur alone before COPD development as a result of α-antitrypsin deficiency (Sherrill et al., 1999;Eden et al., 2006;Eden 2010;Segal and Martinez, 2018). Early diagnosis is crucial and essential to prevent progression of the diseases and to implement preventive measures (Guarnieri, 2017).
The purpose of the present study is to explore simple and economically suitable tool for diagnosis of asthmatic bronchitis. The study revealed the physiological correlations among patients at mild and severe stages as compared to healthy individuals. Therefore, it was suggested that the disease might be detected sooner and diseasespecific interventions could be implemented.

Patients and Methods
Asthmatic bronchitis patients were diagnosed clinically in National Research Centre, Dokki, Giza, Egypt. The diagnosis was confirmed by biochemical indexes. Patients were categorized into patients at mild stage and severe stage based on history and clinical data sheets in addition to the imaging examination. Patients who met clinical diagnostic criteria for asthmatic bronchitis and revised by the subspecialty group of respiratory diseases and approved to obey this diagnostic study were selected according to specific inclusion criteria to be under the current study.

Inclusion Criteria
All patients had symptoms of airflow limitation and fulfilled lung function criteria as set out by the National Heart and Lung Institute/World Health Organization Global Initiative for Asthma guidelines (GINA, 2017) had FEV1/FVC of less than 80% and substantial improvement in FEV1 > 200 ml or 12% of the base line value 15 min after taking 200-400 μg, i.e., 2-4 puffs from a standard metered dose inhaler of nebulized salbutamol and or diurnal variation in PEF > 20%. The specimens (fasting venous blood) were drawn from control (healthy individuals) (21 sample), asthmatic bronchitis patients at mild stage (10 sample) and severe stage (12 sample) then placed into separation gel tube and Ethylene Diamine Tetraacetic Acid-2K (EDTA-2K) anticoagulation tube with each 2 ml tube. Another blood samples were collected and centrifuged at 1500 rpm for 15 min and the clear serum transferred into new eppendorff then stored at -20°C until used.

Major Blood Components
Arterial blood samples were collected with heparinized syringes and immediately analyzed using automated blood gas analyzer (ABL 725, Radiometer Medical ApS, Copenhagen, Denmark) for measuring the blood gases (PH, PCO 2 , PO 2 , SO 2 and HCO 3 -) and the electrolytes (Na + and K + ) at 37 o C. Levels of hemoglobin (HB), white blood cells (WBCs) and platelets (PLT) were quantified in whole blood samples by an automatic blood analyzer (XE 2100 D; Sysmex, Kobe, Japan). The other electrolytes (Ca 2+ , Mg 2+ and P 3+ ) were assayed in serum samples using fully automated system.

Liver and Kidney Functions
Activities of liver enzymes (alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) were assessed kinetically in serum samples. Moreover, levels of urea and creatinin (creat.) were determined using commercially available kits purchased from SPINREACT (Santa Coloma, Spain).

Pulmonary Functions
The lung functions including forced expiratory volume in one second (FEV1), forced vital capacity (FVC), ratio of FEV1 to FVC and maximal expiratory pressure (MEP) were assayed in asthmatic bronchitis patients at mild and severe stages before and after receiving treatment using a spirometry (Spirosift spirometry 5000 FUKUDa NENSHI) according to method suggested by Freire et al. (2008) and modified by Blasi et al. (2013).

Ethical Considerations
All procedures performed in studies involving human participants were in accordance with the ethical standards of Institutional Human Ethical Committee of National Research Centre, Dokki, Giza, Egypt, and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Statistical Analysis
Data were expressed as mean ± standard error (SE) of studied groups using one-way analysis of variance (oneway ANOVA) followed by Bonferroni test. Analysis was carried out by Statistical Package for Social Sciences (SPSS for windows, version 11.0). Values of P < 0.05 regards as significant. Moreover, the statistical positive and negative correlations were evaluated among all determined parameters. Values of P < 0.05 were considered significant correlation and those of P<0.01 were considered highly significant one.

Electrophoretic Protein Patterns
Total protein concentration was determined in the serum samples containing water soluble proteins using bovine serum albumin as standard according to method suggested by Bradford (1976). Concentration of total protein should be equal in all wells. The native proteins were separated electrophoretically through Polyacrylamide Gel Electrophoresis (PAGE) according to the methods described by Hames (1990) and modified recently by Darwesh et al. (2015) who reported that samples, gels and running buffers were lacking sodium dodecyl sulphate. The native bands were stained by Commassie Brilliant Blue G-250 for visualizing protein bands. The relative mobility (Rf) and band percent (B%) of the electrophoretically separated proteins were determined in addition to the molecular weights (Mwts) which were estimated in comparison to marker of standard molecular weights with regularly spaced bands ranging from 6.458 to 195.755 KDa. Furthermore, the lipid and calcium moieties of the native proteins were stained by mean of isoelectrophoresis. The lipid moiety was stained by Sudan Black B (SBB) (Subramaniam and Chaubal 1990). In addition, calcium moiety was stained according to method suggested by Abulyazid et al. (2017) and Abd Elhalim et al. (2017) using Alizarin Red 'S' prepared according method described by Zacharia and Kakati (2004).

Electrophoretic Isoenzymes
Native polyacrylamide gel was stained for detecting the isoenzymes electrophoretically after electrophoretic run.
For catalase (CAT) and peroxidase (POX) patterns, the gels were stained based on methods suggested by Siciliano and Shaw (1976) and Rescigno et al. (1997), respectively.

Data Analysis
The polyacrylamide gel plates were photographed by gel documentation system then analyzed using Quantity One software (Version 4.6.2). The Rf and B% of the electrophoretically separated bands were determined by this program. In addition, the similarity index (SI%) was calculated to compare the physiological state of the asthmatic bronchitis patients at mild and severe stages with respect to control group.

Results
As illustrated in Table 1, no statistical differences was noticed in the major blood components (HB, WBCs and PLT) in asthmatic bronchitis patients at mild and severe stages when compared to healthy individuals.
Liver enzymes (ALT and AST) were significantly (P < 0.05) elevated in asthmatic bronchitis patients at mild and severe stages with respect to control. No differences were noticed statistically between asthmatic bronchitis patients at mild and severe stages. As regards to kidney functions (urea and creat.), it was found that urea was significantly (P < 0.05) elevated in patients at mild and severe stages when compared to control individuals. No statistically differences were noticed in creat. level between asthmatic bronchitis patients at both stages with respect to control.
In addition, the blood gases showed no differences recorded significantly in asthmatic bronchitis patients at both of mild and severe stages when compared to healthy individuals. Furthermore, no statistical variations were noticed in most of the electrolytes (Na + , K + , Mg 2+ and P 3+ ) when compared to healthy individuals. It was found that Ca 2+ level decreased significantly (P < 0.05) in asthmatic bronchitis patients at severe stage when compared to healthy individuals and to patients at mild stage.
The pulmonary functions were not affected before or after receiving treatment in asthmatic bronchitis patients at mild and severe stages. As revealed in Table 2, it was found that all pulmonary functions declined significantly (P < 0.05) in patients at mild and severe stages when compared to healthy individuals. No statistical difference was observed in those functions when patients at mild stage compared to that at severe stage.
As revealed in Fig. 1a, it was noticed that there are 8 types of native proteins identified at Rfs ranged from 0.046 (Mwt 214.04 KDa) to 0.954 (Mwts 6.64 KDa) in sera of the healthy individuals. Five common protein bands were identified at Rfs 0.232, 0.406, 0.493, 0.804 and 0.954 (Mwts 83.862,35.392,25.123,11.124 and 6.637 KDa,respectively). No characteristic protein bands were observed.
In patients at mild stage, it was found that 3 normal protein bands were disappeared with existence of 2 abnormal ones (Rfs 0.073 and 0.123; Mwts 188.705 and 148.11 KDa, respectively). In patients at severe stage, the 2 nd and 3 rd normal proteins were shifted to be identified at Rfs 0.077 and 0.125 (Mwts 185.083 and 146.683, respectively).
From the dendogram and calculations of the SI%, it was found that number and arrangement of the protein bands in the disease at mild stage is physiologically similar to control by 54.5%. Moreover, the disease at severe stage is similar to control by 75.6%. In addition, the patients at mild and severe stages are physiologically similar by 77%.
As presented graphically in Fig. 1b, it was found that 12 common lipid moiety of protein bands were identified at Rfs ranged from 0.061 to 0.958 and B% from 0 to 12.939 in sera of the healthy individuals. There is only one characteristic band noticed in asthmatic bronchitis patients at severe stage at Rf 0.035 (B% 14.109).
No normal bands disappeared in asthmatic bronchitis patients at both mild and severe stages but 2 abnormal bands were identified at mild stage at Rfs 0.179 and 0.496 (B % 8.231 and 5.457, respectively). At the severe stage, 2 abnormal bands were identified at Rfs 0.179 and Table 1 Statistical significance of major blood components (hemoglobin (HB), white blood cells (WBCs) and platelets (PLT)), liver and kidney functions, blood gases and electrolytes showing the differences between asthmatic bronchitis patients at mild and severe stages as compared to control  From dendogram and calculated SI% values, it was found that number and arrangement of bands of lipid moiety at the mild stage is similar to those of control by 73.1%. Moreover, the disease at severe stage is physiologically similar to control by 41.6%. Furthermore, the mild and severe stages of the disease are physiologically similar by 55.8%.
Data illustrated graphically in Fig. 1c showed that 4 common calcium moiety of protein bands were identified at Rfs 0.392, 0.616, 0.845 and 0.954 (B% 12.870, 11.757, 17.736, and 9.690, respectively) in sera of control group. Two characteristic bands were identified in patients at mild stage at Rfs 0.263 and 0.444 (B% 12.234 and 12.444, respectively) with hiding 2 normal bands. As regards to patients at severe stage, it was observed that 4 normal calcium moiety bands were completely disappeared without existence of abnormal ones.
The dendogram and calculated SI% values stated that number and arrangement of bands of calcium moiety in the disease at the mild stage is similar to those of control by 66.4%. Moreover, the disease at severe stage is physiologically similar to control by 30.6%. Moreover, the mild and severe stages of the disease are physiologically similar by 41.5%.
As presented graphically in Fig. 2a, it was found that no statistical variations among all groups in intensity of protein bands. As regards to intensities of lipid and calcium moieties of native proteins, it was noticed that intensities of the lipid and calcium moieties were significantly (P < 0.05) lowered in asthmatic bronchitis patients at both stages when compared to healthy individuals. Moreover, intensities of that moieties decreased significantly (P < 0.05) in patients at severe stages when compared to the mild stage.
As regards to quantities of the native bands (Fig. 2b), it was found that quantities of protein bands decreased significantly (P < 0.05) in patients at mild and severe stages when compared to healthy individuals. With respect to patients at mild stage, quantities of the protein bands lowered significantly (P < 0.05) in patients at severe stage. As regards to quantities of the lipid and calcium moieties of native protein, it was noticed that quantities of the lipid and calcium moieties were significantly (P < 0.05) elevated in asthmatic bronchitis patients at severe stage when compared to the patients at mild stage.
Data illustrated in Fig. 3a, it was noticed that CAT isoenzyme was expressed by 4 normal types which are considered as common types identified at Rfs 0.247, 0.366, 0.755 and 0.962 (B% 22.83, 22.97, 33.40 and 20.80, respectively). No normal bands disappeared in patients at both mild and severe stages but only one characteristic CAT type was identified at 0.146 (B% 17.20) in patients at severe stage. From dendogram and SI% calculations, it was found that number and arrangement of CAT bands at mild and severe stages are similar to those of control by 95.2 and 35.4%, respectively. In addition, the mild and severe stages of the disease are physiologically similar by 32.6%.
As presented graphically in Fig. 3b, it was found that 5 normal types of POX isoenzyme were identified at Rfs 0.059, 0.273, 0.393, and 0.697 (B% 21.88, 19.72, 18.33, 21.77, and 18.30, respectively). There is only one common band identified at Rf 0.393 (B% 21.77). Three normal bands disappeared in asthmatic bronchitis patients at both mild and severe stages but it was noticed that one characteristic band was identified in patients at mild stage at Rf 0.866 (B% 29.79). Moreover, one characteristic band identified in patients at severe stage at Rf 0.181 (B% 32.74).
The dendogram and SI% values showed that number and arrangement of bands of POX isoenzyme in the patients at mild stage is similar to those of control by 40.1%. Moreover, the disease at severe stage is physiologically similar to control by 50.5%. Furthermore, the mild and severe stages of the disease are physiologically similar by 54.9%. Data illustrated graphically in Fig. 3c showed that α-EST isoenzyme was represented by 6 normal types identified at Rfs 0.05, 0.137, 0.37, 0.783, 0.893, and 0.97 (B% 16.94, 16.29, 16.38, 16.33, 17.02, and 17.04, respectively). Four common α-EST types were identified at Rfs 0. 37, 0.783, 0.893, and 0.97 (B% 16.38, 16.33, 17.02 and 17.04, respectively). It was observed that 2 normal α-EST types were disappeared in patients at both stages without existence of abnormal ones.
Values of the calculated SI% postulated that number and arrangement of bands in α-EST isoenzymes in the disease at mild stage is similar to those of control by 67.9%. Moreover, the disease at severe stage is physiologically similar to control by 69.6%. Patients at mild and severe stages are physiologically similar by 93.3%.
As revealed from the dendogram and SI% values, it was found that number and arrangement of bands in β-EST isoenzyme in the disease at mild stage is similar to those of control by 83.1%. Moreover, the disease at severe stage is physiologically similar to control by 73.1%. Patients at mild and severe stages are physiologically similar by 80.5%.
As presented graphically in Fig. 4a, it was found that intensities of CAT and β-EST bands decreased significantly (P < 0.05) in asthmatic bronchitis patients at mild stage. No statistical difference was noticed in intensities of POX and α-EST bands in that disease stage. In patients at severe stage, it was stated that intensities of CAT and α-EST bands increased significantly (P < 0.05) as compared to control and patients at mild stage. No statistical differences were noticed in intensities of POX and β-EST bands in that stage as compared to control and patients at mild stage.
As regards to quantities of isoenzymes bands (Fig. 4b), it was found that quantities of POX bands increased significantly (P < 0.05) in patients at mild stage associated with lowering quantities of β-EST bands significantly (P < 0.05) as compared to healthy individuals. No statistical differences were noticed in quantities of CAT and α-EST bands in patients at that stage. In patients at severe stage, quantities of CAT, α-EST and β-EST bands lowered significantly (P < 0.05) with respect to control. While with respect to patients at mild stage, quantities of CAT, POX and α-EST bands decreased significantly (P < 0.05) associated with elevation of β-EST bands quantities.
Data compiled in Table 3 showed the positive and negative correlations among intensities and quantities of the native electrophoretic patterns in asthmatic bronchitis patients at mild and severe stages before and after receiving treatment.

Discussion
As suggested by Dhahir and Noaman (2017), it was necessary to measure liver enzymes (AST and ALT) and electrolytes in case of pulmonary diseases. These enzymes exhibit their functions by catalyzing transfer of the amino group converting aspartate and alanine into oxaloacetate and pyruvate, respectively. Moreover, the electrolytes play a major role in regulating the body fluids, maintaining normal acid base balance, blood clotting, muscles contractions and nerve conduction. Consequently, the disturbances in the electrolytes lead to hepatic disease, coronary heart disease, renal failure and endocrine disorders (Jay et al., 2000;John, 2007). During the present study, it was found that the liver enzymes were significantly (P < 0.05) elevated in asthmatic bronchitis patients at mild and severe stages with respect to control. This might due to release of high level of the cellular oxidative radicals as supported by findings of Wannamethee and Shaper (Wannamethee and Shaper, 2010), although some of the recent studies argued that the factors affecting pulmonary tissue does not cause damage to hepatocytes directly (Nagao and Sata, 2010).
Markers of kidney functions elevated significantly in asthmatic bronchitis patients. This was in agreement with Huang et al. (2014) who reported that the patients with asthmatic bronchitis may have increased risk of developing chronic kidney disease. This might refer to severe retention of salt and water, reduction in renal blood flow, and the abnormalities in glomerular filtration rate and of water handling that occur in association to the chronic asthmatic bronchitis (Daggett, 1977;Anand et al., 1992).
Arterial blood gases are considered as one of the first critical tests because it is an extremely useful to assess the oxygenation, pH, and PCO 2 giving information about status of oxygenation and ventilation which disturbed seriously in case of severe asthmatic bronchitis (Padmavathi et al., 2013). In the present study, no significant differences recorded in parameters of blood gases in the asthmatic bronchitis patients. These results were supported by findings of İlhan et al. (2016) who documented that there were no significant differences the arterial blood gases in the asthmatic bronchitis. Therefore, the blood gases were not found useful tool for predicting mortality in that patients.
Sodium and potassium assists for maintaining the balance between the body electrolytes and water (Nguen and Kurtz, 2004). The current study showed that there were no significant changes in serum Na + , K + , Mg 2+ and P 3+ in the asthmatic bronchitis patients. This was in accordance with the concept suggested by Al-harbi (2012) who postulated that there were no significant differences in these measurements, since people consume enough salt in diet.
Regarding Ca 2+ level, it was found that Ca 2+ decreased significantly in asthmatic bronchitis patients at severe stage when compared to healthy group and patients at mild stage. This might be attributed to alterations in the Table 3 Significant correlations among intensities and quantities of the native electrophoretic patterns in asthmatic bronchitis patients at mild and severe stages before and after receiving treatment mechanisms that regulate the electrolyte levels inside and outside the cells in response to this deleterious factor leading to loss of the large amounts of Ca 2+ in the urine. Moreover, this factor might affect the parathyroid gland which is responsible for regulating the Ca 2+ level in the serum. This exhibited adverse effect on the body and bone metabolism due to reduction of the Ca 2+ absorption and the bone density (Jorde et al., 2005).
Spirometry plays an important role in evaluation of asthma, including responsiveness to bronchodilators (Backman et al., 1997). The treatment decreased the bronchial hyper-responsiveness and inflammation to prevent further deterioration in the pulmonary indexes (FEV1, FVC, FEV1/FVC %, and MEP) which are considered important parameters for asthma diagnosis and follow-up (Eden et al., 2006;Ciprandi et al., 2012;Guarnieri, 2017;Segal and Martinez, 2018). During the present study, it was found that the pulmonary indexes in patients with asthmatic bronchitis were higher after treatment than those before treatment. This was in agreement with Hou et al. (2018) who reported that these alterations might be attributed to the changes in polymorphism of vitamin D receptor gene which is negatively correlated with severity of asthma.
Proteins are the macromolecules that act as key players inside the cells. They facilitate transporting hormones, vitamins, minerals, lipids and other materials in addition to their effective role for maintaining the delicate blood acid-alkaline balance and balance the osmotic pressure of the blood and tissue (Ali et al., 2006). They circulate to the lungs through the blood stream for protecting that tissue from damage induced by elastases which degrade components of the extracellular matrix (Lewis, 2012). As a result of exposure of the lungs to inflammatory stimuli, elastases expressed severely accelerating asthma and hence lesions of the lung tissue (Crystal, 1990). The serum proteins are susceptible to be oxidized depending on the relative content of oxidationsensitive amino acid residues. Not all proteins are expressed at all times; their expression related to the cellular phenotype and it depends on physiological and environmental factors (Östman et al., 2011).
Protein pattern is a useful tool for diseases diagnosis and the individual serum proteins vary in their physiological functions enable the discovery of diseases biomarkers (O'Connell et al., 2005). The PAGE was used for separation and identifying of different proteins depending on charge, size and shape of protein molecules. Each native band is consisting of group of individual proteins with independent metabolic properties (O' Connell et al., 2005;Shah et al., 2010). This technique was used during the current study to show the adverse effect of asthmatic bronchitis on the different intracellular macromolecules inside the cells. It was noticed that severe alterations detected electrophoretically in number and arrangement of the native proteins. This may refer to the free radicals which cause defect in DNA proteins through the direct modifications in side chains of the amino acids. It was found previously that the amino acids which contain aromatic side chain groups are the most sensitive to the free radicals attack, and these amino acids form carbonyl products irreversibly during the oxidation reaction (Shringarpure et al., 2003;Srinivasan et al., 2006).
Lipoproteins exhibit effective role in carrying all types of lipids, but in different proportions. The serum lipoproteins are susceptible to be oxidized by hydroxyl radicals (Boldyrev et al., 1997). Density of the lipoprotein is directly proportional to the protein content and inversely proportional to the lipid content (Bass et al., 1993). For this reason, the alterations in lipoprotein pattern may refer to the changes in the protein portion.
Calcium moieties of native protein are low molecular weight acidic proteins. They exert inhibitory effect on formation of hydroxyapatite and, consequently, lead to alteration in the mineralization process (Hunter et al., 1996). The alterations in this native pattern result in abnormal mineralization of tissues (Luo et al., 1997). Therefore, the alterations in this native pattern might be correlated to the decline in Ca 2+ level.
The SI% is inversely proportional to the genetic variation. When the SI% value between the abnormal group and the healthy group recorded low values, this indicates to the adverse effect of these substances and the differences in number and arrangement of the protein bands Sharada et al., 2015). The electrophoretic alterations can be represented qualitatively through disappearance of normal bands and appearance of abnormal ones. Otherwise, the alterations may occur at the quantitative level through remaining the normal bands but with changing the band quantity. The SI% is only correlated to the qualitative alterations (Aboulthana et al., 2016).
Due to data of the electrophoretic study, it was found that there were severe alterations in CAT and POX patterns in sera of asthmatic bronchitis patients. This might be occurred due to effect of the free radicals which were able to cause several molecular alterations involving directly in accumulation of genetic changes and depletion of antioxidant enzymes (Conti, 1997). Consequently, this leads to changes in the fractional activity of different isoenzymes correlated with variations in rates of protein expression secondary to DNA damage and hence affecting the isoenzymes (El-Zayat, 2007).
Activity of EST-like albumin used as prognostic markers for various diseases (Thangthaeng et al., 2011). It is expressed as several molecular forms that can be distinguished by their molecular weights and hydrodynamic properties (Massoulié et al., 1993). It can be visualized by substrate staining with using αand βnaphthyl acetate in the presence of Fast Blue RR salt as a dye coupler (Ahmad et al., 2012). During the current study, it was found that the EST pattern was altered qualitatively and quantitatively. This might occur as a result of expression of markers of oxidative stress (Abulyazid et al., 2017;Gangadharan et al., 2007).
The previous studies confirmed that the differences in electrophoretic isoenzymes might be attributable to generation of the free radicals which cause oxidation at level of the nucleic acids. This leads to the formation of various molecular lesions including oxidized bases (purines and pyrimidines), abasic sites (apurinic/apyrimidinic sites) and DNA single-and/or double-strand breaks. Guanine is the most DNA base which is susceptible to be oxidized (Neeley and Essigmann, 2006). Hydroxyl radicals react with pyrimidines (thymine and cytosine) at positions 5 or 6 of the ring producing several lesions (Fujii et al., 2005). If there were no alterations in protein expression, enzymatic activity was not altered (Djordjevic et al., 2010). Moreover, the alterations in the electrophoretic isoenzymes may be attributed to effect of the free radicals which are directly targeting on the nucleic acids (DNA and RNA) responsible for biosynthesis of these enzymes (Javed et al., 2014).

Conclusion
The study concluded that liver and kidney functions were elevated in asthmatic bronchitis patients. No significant differences noticed in the pulmonary indices and arterial blood gases and most of electrolytes (Na + , K + , Mg 2+ and P 3+ ) in all patients. Moreover, Ca 2+ is the only electrolyte decreased at severe stage of the disease.