Table 3 Effect of synthetic seed matrix supplemented with MS medium and PBZ or CCC at different concentrations on shoots number and length, root length, percentage of rooting and acclimatization of encapsulated shoot tips of Asparagus officinalis after eight weeks from culturing
From: Synthetic seeds for in vitro preservation of Asparagus officinalis L.
Matrix composition | Shoots number/explant | Shoot length (cm) | White functional roots (%) | Root length (cm) | Acclimatization (%) |
|---|---|---|---|---|---|
Non-encapsulated shoot tips | 7.38b | 7.89b | 79.17b | 19.60a | 10.0de |
MS medium | 3.25e | 3.78e | 0.00e | 0.00c | 0.0e |
MS medium + 0.5 mg l−1 PBZ | 6.88b | 6.72c | 63.75c | 4.25bc | 27.5d |
MS medium + 1 mg l−1 PBZ | 5.13c | 6.17c | 90.00ab | 5.50bc | 50.0bc |
MS medium + 2 mg l−1PBZ | 3.88de | 3.83e | 95.00a | 7.00bc | 77.5ab |
MS medium + 0.5 mg l-1 CCC | 9.50a | 9.28a | 0.00c | 0.00c | 0.0e |
MS medium + 1 mg l−1 CCC | 6.75b | 7.89b | 22.5d | 10.00b | 37.5cd |
MS medium + 2 mg l−1 CCC | 5.00cd | 5.17d | 20.00d | 4.75bc | 60.0b |