Table 2 Effect of synthetic seed matrix supplemented with MS medium and PBZ or CCC at different concentrations on the germination percentage of encapsulated shoot tips of Asparagus officinalis after different storage durations
From: Synthetic seeds for in vitro preservation of Asparagus officinalis L.
Matrix composition | Storage duration (week) | |||||||
|---|---|---|---|---|---|---|---|---|
0 | 2 | 4 | 8 | 12 | 16 | 20 | 24 | |
Germination percentage (%) | ||||||||
Non-encapsulated shoot tips | 100 | |||||||
MS medium | 100 | 100.0a | 81.0a | 81.00a | 80.0a | 61.0a | 12.5a | 5.0d |
MS medium + 0.5 mg l−1 PBZ | 100 | 88.6b | 50.0d | 50.00c | 27.7d | 25.0d | 10.0d | 0.0f |
MS medium + 1 mg l−1 PBZ | 100 | 78.0c | 45.0e | 45.00c | 25.0e | 25.0d | 8.0d | 2.0e |
MS medium + 2 mg l−1 PBZ | 100 | 73.0d | 30.0f | 30.00d | 24.6e | 23.5d | 5.0d | 2.0e |
MS medium + 0.5 mg l−1 CCC | 100 | 100.0a | 77.0b | 77.00a | 55.5b | 45.4b | 22.0a | 20.0a |
MS medium + mg l−1 CCC 1 | 100 | 100.0a | 75.0b | 72.33ab | 46.0c | 44.4b | 22.0a | 16.0b |
MS medium + mg l−1 CCC 2 | 100 | 100.0a | 66.5c | 66.50b | 45.0c | 36.0c | 16.0b | 12.5c |