Table 1 Effect of synthetic seed matrix supplemented with MS medium and PBZ or CCC at different concentrations on the duration of germination of encapsulated shoot tips of Asparagus officinalis after different storage durations
From: Synthetic seeds for in vitro preservation of Asparagus officinalis L.
Matrix composition | Storage duration (week) | |||||||
|---|---|---|---|---|---|---|---|---|
0 | 2 | 4 | 8 | 12 | 16 | 20 | 24 | |
Duration of germination (days) | ||||||||
Non-encapsulated shoot tips | 3.00e | |||||||
MS medium | 3.33e | 3.33d | 3.67d | 3.00c | 3.00f | 3.00e | 4.00e | 5.00c |
MS medium + 0.5 mg l−1 PBZ | 9.33d | 13.00c | 14.67c | 15.00b | 15.00e | 15.00d | 32.33bc | 35.00b |
MS medium + 1 mg l−1 PBZ | 12.67cd | 15.00bc | 15.00c | 15.00b | 19.00de | 21.00c | 21.00d | 35.00b |
MS medium + 2 mg l−1PBZ | 14.00bc | 17.00bc | 19.00bc | 19.00b | 25.33bc | 31.67b | 31.67c | 36.67b |
MS medium + 0.5 mg l−1 CCC | 9.33d | 15.00bc | 17.00c | 19.00b | 23.67cd | 27.00b | 27.00cd | 36.67b |
MS medium + 1 mg l−1CCC | 17.33b | 18.33b | 22.33ab | 28.33a | 30.00ab | 36.67a | 38.33ab | 40.00ab |
MS medium + 2 mg l−1 CCC | 23.67a | 25.33a | 27.00a | 31.67a | 35.00a | 38.33a | 43.33a | 45.00a |