Table 1 List of primers used throughout the experimental duration, their sequences, amplicon lengths and annealing temperatures
Primers | Positions on the genomes‡ | Sequences (5′–3′) | Amplicon length (bp) | Annealing Temp (°C) | References |
|---|---|---|---|---|---|
P-35S | 7190–7209 7364–7384 | 5′-GCTCCTACAAATGCCATCA-3′ 5′-GATAGTGGGATTGTGCGTCA-3′ | 195 | 57 | Hemmer (1997) |
Cf3-Cr4 | 7313–7333 7411–7435 | 5′-CCACGTCTTCAAAGCAAGTGG-3′ 5′-TCCTCTCCAAATGAAATGAACTTCC-3′ | 123 | 62 | Lipp et al. (2001) |
NPTII | 2382–2397 2554–2539 | 5′-GGATCTCCTGTCATCT-3′ 5′-GATCATCCTGATCGAC-3′ | 173 | 50 | Hemmer (1997) |
NPTII | 2145–2167 2225–2245 | 5′-CTATGACTGGGCACAACAGACA-3′ 5′-CGGACAGGTCGGTCTTGACA-3′ | 101 | 60 | Cited by Li et al. (2015) |
aadA | 1188–1208 1471–1451 | 5′-CGC TAT GTT CTC TTG CTT TTG-3′ 5′-TGA TTT GCT GGT TAC GGT GAC-3′ | 284 | 63 |