In vitro propagation and improving accumulation of coumarin in Lycium barbarum, a rare plant in the flora of Egypt

Bulletin of the National Research Centre

Table 7 Effect of different concentrations of chitosan and cinnamic acid on coumarin content (mg/g fresh weight) in callus and suspension cultures of L. barbarum

Treatments		Conc (μM)	Conc. Of coumarin (%)	Increase (fold)	Decrease (fold)	Callus fresh weight	Callus dry weight
Mother plant (Grow in open field)			0.04388	0.00	0.00	0.00^o	0.0^k
Control			0.0268	0.00	0.00	2.00ⁿ	0.22ⁱ
Callus culture	Chitosan	381	0.00008	0.00	0.003	2.30^m	0.21^j
		763	0.0328	1.22	0.00	3.10^j	0.31^f
		1526	0.0296	1.10	0.00	2.90^k	0.28^g
	Cinnamic acid	381	0.038	1.41	0.00	3.50^f	0.35^d
		763	0.0448	1.67	0.00	3.90^c	0.41^b
		1526	0.0424	1.58	0.00	3.30^h	0.33^e
Suspension culture	Control		0.0356	1.32	0.00	2.50^l	0.25^h
	Chitosan	381	0.04372	1.63	0.00	3.40^g	0.35^d
		763	0.0496	1.85	0.00	4.10^b	0.41^b
		1526	0.042	1.56	0.00	3.20ⁱ	0.31^f
	Cinnamic acid	381	0.044	1.64	0.00	3.80^e	0.39^c
		763	0.0524	1.95	0.00	4.40^a	0.45^a
		1526	0.046	1.71	0.00	3.82^d	0.39^c

Means followed by the same letter are not significantly different according to Duncan’s multiple range test. The mean difference is significant at the 0.05 level. The presence of different letters in the same table indicates a high significance between the numbers. The higher significance between the results obtained, this leads to the numbers taking different letters