In vitro propagation and improving accumulation of coumarin in Lycium barbarum, a rare plant in the flora of Egypt

Bulletin of the National Research Centre

Table 5 The effect of TDZ concentrations on direct organogenesis formation from L. barbarum leaf explants

Treatments (µM)		Mean number of adventitious buds/explant	Frequency of shoot regeneration
Control		2^j	1^l
TDZ	22.02	2^j	5^k
	44.05	3ⁱ	10^j
	66.07	5^h	15ⁱ
	88.10	6^g	20^h
	110.12	8^f	25^g
	132.15	9^e	70^f
	154.17	10^d	80^e
	176.20	11^c	90^c
	198.22	13^b	92^b
	220.25	15^a	96^a
	242.27	10^d	85^d
	264.30	9^e	70^f

Means followed by the same letter are not significantly different according to Duncan’s multiple range test. The mean difference is significant at the 0.05 level. The presence of different letters in the same table indicates a high significance between the numbers. The higher significance between the results obtained, this leads to the numbers taking different letters