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Bulletin of the National Research Centre
Fig. 8 | Bulletin of the National Research Centre

Fig. 8

From: In vitro propagation and improving accumulation of coumarin in Lycium barbarum, a rare plant in the flora of Egypt

Fig. 8Fig. 8

HPLC chromatograph shows the peak of coumarin from leaf callus of L. barbarum cultured on MS callus proliferation medium containing 552.60 µM TDZ under total darkness. 1 = Mother plant (grown in open field). 2 = Callus culture without additives (control). 3 = Callus culture with 381 μM chitosan. 4 = Callus culture with 763 μM chitosan. 5 = Callus culture with 1526 μM chitosan. 6 = Callus culture with 381 μM cinnamic acid. 7 = Callus culture with 763 μM cinnamic acid. 8 = Callus culture with 1526 μM cinnamic acid. 9 = Callus culture without additives (control). 10 = Suspension culture with 381 μM chitosan. 11 = Suspension culture with 763 μM chitosan. 12 = Suspension culture with 1526 μM chitosan. 13 = Suspension culture with 381 μM cinnamic acid. 14 = Suspension culture with 763 μM cinnamic acid. 15 = Suspension culture with 1526 μM cinnamic acid

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