Direct detection of iro B, stn and hil A virulence genes in Salmonella enterica serovar typhimurium from non-ripened cheese

Bulletin of the National Research Centre

Table 1 PCR primer pairs screened for specificity to Salmonella enterica

Primer pair target	Primer sequence (5’ → 3’) (*)	Annealing†	Cycles‡	Length§
SPI1 invasion gene (hil A)	CTG CCG CAG TGT TAA GGA TA CTG TCG CCT TAA TCG CAT GT	62	30	497
Enterotoxin gene (stn)	CTT TGG TCG TAA AAT AAG GCG TGC CCA AAG CAG AGA GAT TC	55	25	260
Fur-regulated gene (iro B)	TGC GTA TTC TGT TTG TCG GTC C TAC GTT CCC ACC ATT CTT CCC	55	30	606
Multiplex – virulence plasmid gene (spvC)	ACT CCT TGC ACA ACC AAA TGC GGA TGT CTT CTG CAT TTC GCC ACC ATC A			571

^*The Primer sequence shows forward primer listed first followed by the reverse primer
^†Annealing temperature in ̊C
^‡Cycles = the number of times the dissociation, annealing and elongation steps were repeated for amplification
^§Length of amplification product in base pairs