From: Use of the leaves of Alchornea cordifolia (Schumach. & Thonn.) Müll (Euphorbiaceae) and prospects for treatment of infections due to multidrug-resistant bacteria
Step
Temperature (°C)
Time (min)
Number of cycles
Initial denaturation
94
4
–
Denaturation
1
30
Hybridization
50
Elongation
72
Final elongation
5