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Table 3 PCR parameters for Mec A

From: Use of the leaves of Alchornea cordifolia (Schumach. & Thonn.) Müll (Euphorbiaceae) and prospects for treatment of infections due to multidrug-resistant bacteria

Step

Temperature (°C)

Time (min)

Number of cycles

Initial denaturation

94

4

Denaturation

94

1

30

Hybridization

50

1

 

Elongation

72

1

 

Final elongation

72

5

  1. -: not considered