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Bulletin of the National Research Centre

Table 2 PCR conditions for Bla-TEM, SHV, CTM-1 and CTM-15

From: Use of the leaves of Alchornea cordifolia (Schumach. & Thonn.) Müll (Euphorbiaceae) and prospects for treatment of infections due to multidrug-resistant bacteria

Step

Temperature (°C)

Time

Number of cycles

Initial denaturation

95 °C

4 min

–

Denaturation

94 °C

30 s

30

Annealing

68 °C

1 min

 

Extension

68 °C

1 min

 

Final extension

72 °C

5 min

–

  1. –: not considered
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