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Bulletin of the National Research Centre

Table 1 Nucleotide primers used for amplification of nitrilase gene

From: Nitrilase gene detection and nitrile metabolism in two bacterial strains associated with waste streams in Lagos, Nigeria

Primers

Sequence

Tm (°C)

%GC

Σ base (bp)

Bac-Nit-F

5′-GAC AAG TAT TTA CCC GAA GTT TCG-3′

55

24

24

Bac-Nit-R

5′-GAT ATT GAA TAG CCT CGT ATG-3′

55

38

21

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