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Table 1 Differences in NO, IL-6, and leptin amounts quantified between macrophage-containing transwell inserts and adipocyte-containing wells (average of all treatments)

From: Effects of co-incubation of LPS-stimulated RAW 264.7 macrophages on leptin production by 3T3-L1 adipocytes: a method for co-incubating distinct adipose tissue cell lines

Molecule

Average amount of molecule in transwell (macrophages)

Average amount of molecule in well (adipocytes)

Difference between transwell and well (matched pairs t test)

NO (mM)

7.2 ± 1.0

6.0 ± 1.0

p = 0.26

IL-6 (pg/ml)

1172.2 ± 122.0

1115.76 ± 122.0

p = 0.65

Leptin (pg/ml)

478.2 ± 47.5

627.1 ± 47.5

p = 0.004*

  1. Matched pairs t tests with p < 0.05 indicate significant difference in amount of molecule measured in transwells and wells