Fig. 4
From: In vitro antioxidants and antihypertensive properties of corn silk–lemon infusion
Angiotensin-I-converting enzyme (ACE) inhibition of corn silk–lemon and commercial tea infusion. Angiotensin-I-converting enzyme (ACE) inhibition assay in corn silk–lemon and commercial tea infusion by a spectrophotometric method of Cushman and Cheung (1971). The substrate [Hippuryl-histidyl-leucine (Bz-Gly-His-Leu)] and ACE (EC 3.4.15.1) was obtained from rat penile tissue. The amount of cleaved hippuric acid from hippuryl-histidyl-leucine was measured by the enzymatic method. The absorbance of the mixture was measured at 228 nm. The control experiment was performed without the test sample and the ACE inhibitory activity was expressed as percentage inhibition and plotted against five samples extract concentration (ug/ml). CSF: corn silk flour; LF: lemon flour; A: 100% CSF; B: 95% CSF and 5% LF; F: top tea; L: lipton tea; G: green tea