Table 2 Purification steps of l-glutaminase produced by Bacillus sp. DV2-37
From: Production, characterization, and antitumor efficiency of l-glutaminase from halophilic bacteria
Purification Procedure | Enzyme Activity (U) | Protein (mg) | Specific activity (U/mg) | Fold purification | Yield (%) |
|---|---|---|---|---|---|
Cell free extract | 1336 | 565 | 30.23 | 1.0 | 100 |
Ammonium sulphate | 9871 | 225 | 53.07 | 1.75 | 75.19 |
Dialysis | 7053 | 58 | 176.2 | 5.8 | 51.15 |