Table 1 Summary of actinidin purification from Actinidia deliciosa Chev
Purification step | Volume (ml) | Activity (U/ml) | Protein (mg/ml) | Total protein (mg) | Total enzyme activity(U) | Specific activity (U/mg) | Purification (fold) | Recovery (% yield) |
|---|---|---|---|---|---|---|---|---|
CE | 452 | 59.67 | 1.05 | 475.48 | 26,970 | 56.82 | 1.00 | 100.00 |
PP | 26 | 244.38 | 2.76 | 71.86 | 6353 | 88.54 | 1.55 | 23.55 |
DEAE Sepharose | 15 | 403.52 | 2.93 | 43.95 | 6052 | 137.72 | 2.42 | 22.43 |