Table 1 List of primers employed for detection and quantification of GMOs, Primers positionsa on the genomes, sequences, amplicon lengths and annealing temperatures
Primers | Positions on the genomesa | Sequences (5′–3′) | Amplicon length (bp) | Annealing temp. (°C) | References |
|---|---|---|---|---|---|
P-35S | 7190–7209 | 5′–GCTCCTACAAATGCCATCA–3′ | 195 | 57 | Hemmer (1997) |
7364–7384 | 5′–GATAGTGGGATTGTGCGTCA–3′ | ||||
GT88 | 7117–7138 | 5′–TCCGGAAACCTCCTCGGATTCCAT–3′ | 88 | 54 | Oraby et al. (2014) |
7183–7206 | 5'–GGCATTTGTAGGAGCCACCTTCCT–3' | ||||
nptII | 2382–2397 | 5′–GGATCTCCTGTCATCT–3′ | 173 | 50 | Hemmer (1997) |
2539–2554 | 5′–GATCATCCTGATCGAC–3′ | ||||
nptII | 2145–2167 | 5′–CTATGACTGGGCACAACAGACA–3′ | 101 | 60 | Cited by Li et al. (2015) |
2225–2245 | 5′–CGGACAGGTCGGTCTTGACA–3′ | ||||
Probe: 2172–2195 | FAM–CTGCTCTGATGCCGCCGTGTTCCG–TAMRA | ||||
aphIV | 14,025–14,044 | 5′–CCGATTCCGGAAGTGCTTGA–3′ | 649 | 60.5 | Designed for this work |
14,654–14,673 | 5′–CCCAAGCTGCATCATCGAAA–3′ | ||||
aadA | 1188–1208 | 5′–CGC TAT GTT CTC TTG CTT TTG–3′ | 284 | 63 | Hollingshead and Vapnek (1985) |
1451–1471 | 5′–TGA TTT GCT GGT TAC GGT GAC–3′ |